Bacillus spp.-Mediated Growth Promotion of Rice Seedlings and Suppression of Bacterial Blight Disease under Greenhouse Conditions
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacillus Strains and Their Growth Conditions
2.2. Phytopathogens Used in the Study
2.3. In Vitro Antagonistic Activity of Bacillus Strains against Bacterial and Fungal Phytopathogens
2.4. Identification of Potential Antagonists
2.5. Effect of Bacillus spp. on the Growth of Rice Seedlings
2.6. Suppression of BB of Rice by Potential Bacillus spp.
BB Inoculation and Evaluation
2.7. Bacillus Strains Colonization of Rice Roots
2.8. Analysis of Lipopeptides by MALDI-TOF MS
2.9. Data Analysis
3. Results and Discussion
3.1. In Vitro Broad-Range Antagonistic Activity of Bacillus Strains
3.2. Identification of Potential Antagonists
3.3. Growth Promotion of Rice Seedlings by Potential Bacillus spp.
3.4. Suppression of BB Disease by Potential Bacillus spp. under Greenhouse Conditions
3.5. Colonization of FZB42, FA12, and FA26 on Rice Roots
3.6. Identification of Antimicrobial Lipopeptides from Bacillus spp.
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Treatments | Germination (%) | Seedling Length (cm) | Vigor Index | Wet Weight (mg) | Dry Weight (mg) | Disease Incidence (%) | Suppression Efficacy (%) | CFU/g Rhizospheric Soil Sample* | ||
---|---|---|---|---|---|---|---|---|---|---|
Shoot | Root | Relative Increase (%) | ||||||||
FZB42 | 95 | 5.7 ± 0.79b | 10.1 ± 1.43a | 1501.0c | 73.73 | 768b | 510b | 10.38 ± 2.76b | 68.44a | (2.2 ± 1.3) × 105a |
FA12 | 92 | 5.9 ± 0.57b | 10.9 ± 0.74a | 1545.6b | 78.89 | 892a | 617a | 13.24 ± 1.77b | 59.74b | (2.0 ± 0.5) × 105a |
FA26 | 98 | 6.7 ± 0.26a | 11.7 ± 0.80a | 1803.2a | 108.70 | 834ab | 639a | 14.90 ± 1.38b | 54.70c | (1.7 ± 1.6) × 105a |
Control | 72 | 4.4 ± 0.20c | 7.6 ± 1.80b | 864.0d | – | 459c | 262c | 32.89 ± 2.14a | – | – |
Mass Peaks (m/z) | Assignment |
---|---|
(1) IturinA (strain: FA12) | |
1065.5 1079.5/1095.5 1109.5 | C14-IturinA [M + Na]+ C15-IturinA [M + Na, K]+ C16-IturinA [M + K]+ |
(2) Fengycin (strains: FA12; FA26) | |
1463.9/1485.8 | C14-fengycin [M + H, Na]+ |
1477.9/1499.8 | C15-fengycin [M + H, Na]+ |
1491.8/1513.8 | C16-fengycin [M + H, Na]+ |
1505.8/1529.8 | C17-fengycin [M + H, Na]+ |
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Rajer, F.U.; Samma, M.K.; Ali, Q.; Rajar, W.A.; Wu, H.; Raza, W.; Xie, Y.; Tahir, H.A.S.; Gao, X. Bacillus spp.-Mediated Growth Promotion of Rice Seedlings and Suppression of Bacterial Blight Disease under Greenhouse Conditions. Pathogens 2022, 11, 1251. https://0-doi-org.brum.beds.ac.uk/10.3390/pathogens11111251
Rajer FU, Samma MK, Ali Q, Rajar WA, Wu H, Raza W, Xie Y, Tahir HAS, Gao X. Bacillus spp.-Mediated Growth Promotion of Rice Seedlings and Suppression of Bacterial Blight Disease under Greenhouse Conditions. Pathogens. 2022; 11(11):1251. https://0-doi-org.brum.beds.ac.uk/10.3390/pathogens11111251
Chicago/Turabian StyleRajer, Faheem Uddin, Muhammad Kaleem Samma, Qurban Ali, Waleed Ahmed Rajar, Huijun Wu, Waseem Raza, Yongli Xie, Hafiz Abdul Samad Tahir, and Xuewen Gao. 2022. "Bacillus spp.-Mediated Growth Promotion of Rice Seedlings and Suppression of Bacterial Blight Disease under Greenhouse Conditions" Pathogens 11, no. 11: 1251. https://0-doi-org.brum.beds.ac.uk/10.3390/pathogens11111251