Intestinal and Mucosal Microbiome Response to Oral Challenge of Enterotoxigenic Escherichia coli in Weaned Pigs
Round 1
Reviewer 1 Report
Comments to the Authors of manuscript number: pathogens-1535701 entitled “Intestinal and mucosal microbiomic response to oral challenge of enterotoxigenic Escherichia coli in weaned pigs”.
The authors have presented a study involved neonatal male piglets challenged by enterotoxigenic E. coli. Authors have performed histomorphometrical analysis of ileum as well as proteomic and transcription analyses. However, if piglets have presented diarrhea, I do not know how Authors have found the whole villi for measurements.
- L 13 – what is mucosal microbiome?
- L 49, 51 – what is mucosal microbiome. The first part of introduction relates to gut microbiome. Authors do not explain and present the division of gut microbiome. It should be corrected.
Author Response
Please see the attachment
Author Response File: 
Author Response.pdf
Reviewer 2 Report
The aim of the research was to investigate change of mucosaln microbiome and protein expression in the ileum induced by E. coli K88 (ETEC) using pigs as a model. The number samples used in the experiment is sufficient. The applied research methods are correct. The discussion is well conducted and comprehensive. Well-chosen references. References must be made in accordance with the instructions for authors. Before publishing in Pathogens, the article requires additions and corrections. The proposed changes are listed below:
General comments:
- For affiliates, the email for each co-author of the article should be provided
- For affiliates, the first name and surname initials for each co-author of the article should be provided, the same as given in the "Author Contributions" chapter.
The "Author Contributions" chapter must follow the instructions: activity 1 name, authors, activity 2, authors, etc.
The References section must follow the instructions for authors. A template from the Pathogens journal manual is provided below:
- Author 1, A.B.; Author 2, C.D. Title of the article. Abbreviated Journal Name Year, Volume, page range.
- Author 1, A.; Author 2, B. Title of the chapter. In Book Title, 2nd ed.; Editor 1, A., Editor 2, B., Eds.; Publisher: Publisher Location, Country, 2007; Volume 3, pp. 154–196.
- Author 1, A.; Author 2, B. Book Title, 3rd ed.; Publisher: Publisher Location, Country, 2008; pp. 154–196.
- Author 1, A.B.; Author 2, C. Title of Unpublished Work. Abbreviated Journal Name year, phrase indicating stage of publication (submitted; accepted; in press).
- Author 1, A.B. (University, City, State, Country); Author 2, C. (Institute, City, State, Country). Personal communication, 2012.
- Author 1, A.B.; Author 2, C.D.; Author 3, E.F. Title of Presentation. In Proceedings of the Name of the Conference, Location of Conference, Country, Date of Conference (Day Month Year).
- Author 1, A.B. Title of Thesis. Level of Thesis, Degree-Granting University, Location of University, Date of Completion.
- Title of Site. Available online: URL (accessed on Day Month Year).
For example, for original papers it must be Abbreviated name journal in italic, rok in bold, volume number in italic, page range using a long "-" from the Insert Symbol function
When describing the meaning, use lowercase "p" in italics, spaces before and after "<" for example (p < 0.05)
Deatiled comments:
L14 pigs as a model, add "a", E. coli in italic
L18 TLR9 also
L21 p in ilatlic
L39 space before [10]
L56 + Please include Figure S1 and Table S1 in the main article, not in supplements
L74,76 p in italic
L78 fatty with small letters
Figure 1, Please add description of A, B, C, D, E for each graph
Figure 2 I propose to increase the size, vertical one below the other, 3 charts A, B, C on 1 page
L119 r = 0.96 not 0.9
L120 r = -0.74
L122 r = -0.92 not -0.9
L205 add information about the type of building, type of floor, pen size, % of humidity, lighting program, type of light
L293 p < 0.05; instead of p value 0.05
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
This manuscript is a resubmission of an earlier submission. The following is a list of the peer review reports and author responses from that submission.
