Effects of Glycyrrhizin on Multi-Drug Resistant Pseudomonas aeruginosa
Abstract
:1. Introduction
2. Results
2.1. Proteomics
2.2. Bacterial Adherence to Mouse Corneal Epithelial Cells (MCEC) and Human Corneal Epithelial Cells (HCET)
2.3. Biofilm and Dispersal with GLY
3. Discussion
4. Materials and Methods
4.1. Bacterial Culture
4.2. GLY Treatment For Proteomics
4.3. Proteomics
4.4. Bacterial Adherence Assay
4.5. Biofilm Dispersal by Glycyrrhizin
4.6. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Set Name | Proteins in Set | GLY vs. ctrl MDR9 | GLY vs. ctrl B1045 | MDR9 vs. B1045 |
---|---|---|---|---|
Antibiotic Resistance | 2 | * −5.12 | 0.38 | * 11.98 |
Biofilms/Adherence | 17 | * −1.77 | 0.32 | −0.63 |
Virulence/Quorum | 7 | −0.77 | 0.99 | −0.31 |
Set ID | Description | Score * | FDR | Proteins in Set | Similar Pathways |
---|---|---|---|---|---|
CL:7881 | mixed, incl.Biosynthesis of siderophore group | ||||
nonribosomal peptides, and Acetyl-coenzyme A | −5.86 | 3.07 × 10−6 | 8 | 15 | |
transporter 1 | |||||
CL:5839 | mixed, incl Bacterial secretion system, | ||||
and Tir chaperone protein (CesT) family | −3.32 | 4.61 × 10−6 | 13 | 14 | |
CL:126 | Ribosome | 1.28 | 6.91 × 10−6 | 48 | 17 |
CL:1605 | Citrate cycle (TCA cycle) | 2.19 | 6.40 × 10−4 | 16 | 4 |
CL:2538 | Quinone, and Thioredoxin-like [2Fe-2S] | ||||
ferredoxin | 2.62 | 8.10 × 10−4 | 11 | 3 |
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Carruthers, N.J.; McClellan, S.A.; Somayajulu, M.; Pitchaikannu, A.; Bessert, D.; Peng, X.; Huitsing, K.; Stemmer, P.M.; Hazlett, L.D. Effects of Glycyrrhizin on Multi-Drug Resistant Pseudomonas aeruginosa. Pathogens 2020, 9, 766. https://0-doi-org.brum.beds.ac.uk/10.3390/pathogens9090766
Carruthers NJ, McClellan SA, Somayajulu M, Pitchaikannu A, Bessert D, Peng X, Huitsing K, Stemmer PM, Hazlett LD. Effects of Glycyrrhizin on Multi-Drug Resistant Pseudomonas aeruginosa. Pathogens. 2020; 9(9):766. https://0-doi-org.brum.beds.ac.uk/10.3390/pathogens9090766
Chicago/Turabian StyleCarruthers, Nicholas J., Sharon A. McClellan, Mallika Somayajulu, Ahalya Pitchaikannu, Denise Bessert, Xudong Peng, Kylie Huitsing, Paul M. Stemmer, and Linda D. Hazlett. 2020. "Effects of Glycyrrhizin on Multi-Drug Resistant Pseudomonas aeruginosa" Pathogens 9, no. 9: 766. https://0-doi-org.brum.beds.ac.uk/10.3390/pathogens9090766