An Experimental Murine Model to Assess Biofilm Persistence on Commercial Breast Implant Surfaces
Abstract
:1. Background
2. Material and Methods
2.1. Bacterial Strain
2.1.1. Implant Surfaces
2.1.2. Implant Colonization
2.2. Experimental Model
2.3. Statistical Analysis
3. Results
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Implant Type | Implant | Capsule | ||
---|---|---|---|---|
Short-Term | Long-Term | Short-Term | Long-Term | |
Smooth, n (%) | 5 (18.5) | 1 (2.8) | 6 (22.2) | 1 (2.8) |
Microtexturized, n (%) | 10 (37) | 1 (2.8) | 10 (37) | 1 (2.8) |
Macrotexturized, n (%) | 24 (88.9) | 17 (47.2) | 22 (81.5) | 11 (30.6) |
Total positive cultures | 39 (48.1) | 19 (17.6) | 38 (46.9) | 13 (12.1) |
Total samples, n (%) | 81 (100) | 108 (100) | 81(100) | 108 (100) |
Total animals, n | 27 | 36 | 27 | 36 |
Infection Stage | Implant | Capsule |
---|---|---|
Short term | ||
Smooth, median (IQR) | 6.4 (5.3–7) | 8.6 (6.9–9.1) |
Microtexturized, median (IQR) | 10.8 (8.9–12) | 12.4 (9.3–13.1) |
Macrotexturized, median (IQR) | 8.8 (6.8–10.4) | 8.3 (5.7–11.2) |
Long term | ||
Smooth, median (IQR) | 3.9 (3.9–3.9) | 2.3 (2.3–2.3) |
Microtexturized, median (IQR) | 6.8 (6.8–6.8) | 5.3 (5.3–5.3) |
Macrotexturized, median (IQR) | 6.4 (4.6–7.5) | 6.2 (3.9–6.8) |
Infection Stage | Implants | |||
---|---|---|---|---|
Friedman Test | p-Value Unadjusted for Multiple Comparisons | SIDAK Post-Hoc Test | HOLM Post-Hoc Test | |
Short-term | ||||
Microtexturized vs. Smooth (p) | <0.001 | 0.065 | 0.184 | 0.065 |
Macrotexturized vs. Smooth (p) | <0.001 | <0.001 | <0.001 | |
Macrotexturized vs. Microtexturized (p) | 0.007 | 0.02 | 0.013 | |
Long-term | ||||
Microtexturized vs. Smooth (p) | 0.005 | 1.0 | 1.0 | 1.0 |
Macrotexturized vs. Smooth (p) | <0.001 | <0.001 | <0.001 | |
Macrotexturized vs. Microtexturized (p) | <0.001 | 0.002 | 0.001 |
Implant Types | Infection Stage | Capsule Weight |
---|---|---|
Smooth, median (IQR) | Short term (n = 27) | 0.5162 (0.2309–0.643) |
Long term (n = 36) | 0.2341 (0.206–0.3176) | |
Microtexturized, median (IQR) | Short term (n = 27) | 0.5569 (0.4367–0.9367) |
Long term (n = 36) | 0.3135 (0.2524–0.4039) | |
Macrotexturized, median (IQR) | Short term (n = 27) | 0.7582 (0.4323–0.9056 |
Long term (n = 36) | 0.31315 (0.25525–0.41565) |
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Carmona-Torre, F.; Fernández-Ciriza, L.; Berniz, C.; Gomez-Martinez de Lecea, C.; Ramos, A.; Hontanilla, B.; del Pozo, J.L. An Experimental Murine Model to Assess Biofilm Persistence on Commercial Breast Implant Surfaces. Microorganisms 2022, 10, 2004. https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms10102004
Carmona-Torre F, Fernández-Ciriza L, Berniz C, Gomez-Martinez de Lecea C, Ramos A, Hontanilla B, del Pozo JL. An Experimental Murine Model to Assess Biofilm Persistence on Commercial Breast Implant Surfaces. Microorganisms. 2022; 10(10):2004. https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms10102004
Chicago/Turabian StyleCarmona-Torre, Francisco, Leire Fernández-Ciriza, Carlos Berniz, Cristina Gomez-Martinez de Lecea, Ana Ramos, Bernardo Hontanilla, and Jose L. del Pozo. 2022. "An Experimental Murine Model to Assess Biofilm Persistence on Commercial Breast Implant Surfaces" Microorganisms 10, no. 10: 2004. https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms10102004