Molecular Diagnosis as an Alternative for Public Health Surveillance of Leptospirosis in Colombia
Abstract
:1. Introduction
2. Disease Burden and Underreporting
3. Diagnosis
3.1. Microscopic Examination
3.2. Culture
3.3. Enzyme-Linked Immunosorbent Assay
3.4. Indirect Immunofluorescence
3.5. Rapid Tests
3.6. Microagglutination (MAT)
3.7. Molecular Diagnostics
4. Leptospirosis in Colombia
4.1. Statistical Calculations
4.2. Regulatory Framework
5. Proposal to Improve the Diagnosis of Leptospirosis in Colombia
6. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Test | Price (COP/US) | Advantages | Disadvantages |
---|---|---|---|
ELISA | $80,000 */20 |
|
|
MAT | $438,000/105 |
|
|
Conventional PCR | $180,000 **/45 |
|
|
Real-time PCR | $250,000 **/60 |
|
|
Year | National Death Rate per 100,000 | Case Fatality Rate (%) | YLPP | YLPP Index | WYL |
---|---|---|---|---|---|
2010 | 0.074 | 2.67 | 1022.86 | 2.32 | 616 |
2011 | 0.072 | 2.58 | 848.12 | 1.90 | 434 |
2012 | 0.056 | 2.40 | 1059 | 2.35 | 648 |
2013 | 0.051 | 2.65 | 537.53 | 1.18 | 253 |
2014 | 0.059 | 2.88 | 965.64 | 2.10 | 646 |
2015 | 0.028 | 1.66 | 408.89 | 0.88 | 215 |
2016 | 0.034 | 2.40 | 429.68 | 0.91 | 204 |
2017 | 0.027 | 1.89 | 527.09 | 1.11 | 335 |
2018 | 0.012 | 1.06 | 231.66 | 0.48 | 133 |
2019 | 0.006 | 3.37 | 97.87 | 0.19 | 57 |
2020 | 0.004 | 6.25 | 72.92 | 0.14 | 46 |
2021 | 0.010 | 4.27 | - * | - * | 77 |
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Share and Cite
Arboleda, M.; Mejía-Torres, M.; Posada, M.; Restrepo, N.; Ríos-Tapias, P.; Rivera-Pedroza, L.A.; Calle, D.; Sánchez-Jiménez, M.M.; Marín, K.; Agudelo-Flórez, P. Molecular Diagnosis as an Alternative for Public Health Surveillance of Leptospirosis in Colombia. Microorganisms 2023, 11, 2759. https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms11112759
Arboleda M, Mejía-Torres M, Posada M, Restrepo N, Ríos-Tapias P, Rivera-Pedroza LA, Calle D, Sánchez-Jiménez MM, Marín K, Agudelo-Flórez P. Molecular Diagnosis as an Alternative for Public Health Surveillance of Leptospirosis in Colombia. Microorganisms. 2023; 11(11):2759. https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms11112759
Chicago/Turabian StyleArboleda, Margarita, Mariana Mejía-Torres, Maritza Posada, Nicaela Restrepo, Paola Ríos-Tapias, Luis Alberto Rivera-Pedroza, David Calle, Miryan M. Sánchez-Jiménez, Katerine Marín, and Piedad Agudelo-Flórez. 2023. "Molecular Diagnosis as an Alternative for Public Health Surveillance of Leptospirosis in Colombia" Microorganisms 11, no. 11: 2759. https://0-doi-org.brum.beds.ac.uk/10.3390/microorganisms11112759