LINCS Dataset-Based Repositioning of Dutasteride as an Anti-Neuroinflammation Agent
Abstract
:1. Introduction
2. Materials and Methods
2.1. Signature Matching and BBB Penetration Prediction
2.2. Cell Culture and Drug Administration
2.3. Cell Viability
2.4. ELISA Measurement of TNF-α and IL-6
2.5. Animals and Drug Administration
2.6. Tissue and Blood Samples Collection
2.7. MWM Test
2.8. Immunofluorescence
2.9. Luminex Assay Detection of Multiple Cytokines
2.10. Statistical Analysis
3. Results
3.1. Repurposing of Dutasteride to Treat Neuroinflammation by Signature Matching and BBB Penetration Screening
3.2. Dutasteride Reduced Cytokine Secretions in LPS-Activated BV2 Microglial Cells
3.3. Dutasteride Decreased Level of IL-6 in Plasma and Hippocampus following LPS Administration
3.4. Dutasteride Decreased Microglia Activation in the Brain of LPS Stimulated Mice
3.5. Effects of Dutasteride on Spatial Learning and Memory in LPS-induced Neuroinflammatory Mouse Model
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Luo, D.; Han, L.; Gao, S.; Xiao, Z.; Zhou, Q.; Cheng, X.; Zhang, Y.; Zhou, W. LINCS Dataset-Based Repositioning of Dutasteride as an Anti-Neuroinflammation Agent. Brain Sci. 2021, 11, 1411. https://0-doi-org.brum.beds.ac.uk/10.3390/brainsci11111411
Luo D, Han L, Gao S, Xiao Z, Zhou Q, Cheng X, Zhang Y, Zhou W. LINCS Dataset-Based Repositioning of Dutasteride as an Anti-Neuroinflammation Agent. Brain Sciences. 2021; 11(11):1411. https://0-doi-org.brum.beds.ac.uk/10.3390/brainsci11111411
Chicago/Turabian StyleLuo, Dan, Lu Han, Shengqiao Gao, Zhiyong Xiao, Qingru Zhou, Xiaorui Cheng, Yongxiang Zhang, and Wenxia Zhou. 2021. "LINCS Dataset-Based Repositioning of Dutasteride as an Anti-Neuroinflammation Agent" Brain Sciences 11, no. 11: 1411. https://0-doi-org.brum.beds.ac.uk/10.3390/brainsci11111411