A Novel Dual-Function Nitric Oxide Donor Therapy for Preeclampsia—A Proof-of-Principle Study in a Murine Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Vascular Function Studies
2.3. Animal Model and AKT-1005 Treatment
2.4. Urine Collections
2.5. Light and Electron Microscopy
2.6. Blood Pressure Measurement
2.7. Enzyme-Linked Immunosorbent Assay (ELISA)
2.8. Statistical Analysis
3. Results
3.1. AKT-1005 Has Vasodilating Effect on Resistance Vessels Ex Vivo and Does Not Cause Hypotension in Control Mice
3.2. AKT-1005 Increases NO Bioavailability and Ameliorates Hypertension in Ad.sFlt-1-Transfected Mice
3.3. AKT-1005 Prevents Renal Glomerular Endotheliosis and Improves Kidney Function in Ad.sFlt-1-Transfected Mice
3.4. AKT-1005 Relieves Placental Stress in Ad.sFlt-1-Transfected Mice
3.5. AKT-1005 Reduces Anti-Angiogenic Response in Ad.sFlt-1-Transfected Mice
3.6. AKT-1005 Does Not Alter Pregnancy Outcomes in Ad.sFlt-1-Transfected Mice
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Pintye, D.; Sziva, R.E.; Biwer, L.A.; Karreci, E.S.; Jacas, S.; Mastyugin, M.; Török, M.; Young, B.C.; Jagtap, P.; Southan, G.J.; et al. A Novel Dual-Function Nitric Oxide Donor Therapy for Preeclampsia—A Proof-of-Principle Study in a Murine Model. Antioxidants 2023, 12, 2036. https://0-doi-org.brum.beds.ac.uk/10.3390/antiox12122036
Pintye D, Sziva RE, Biwer LA, Karreci ES, Jacas S, Mastyugin M, Török M, Young BC, Jagtap P, Southan GJ, et al. A Novel Dual-Function Nitric Oxide Donor Therapy for Preeclampsia—A Proof-of-Principle Study in a Murine Model. Antioxidants. 2023; 12(12):2036. https://0-doi-org.brum.beds.ac.uk/10.3390/antiox12122036
Chicago/Turabian StylePintye, Diana, Réka E. Sziva, Lauren A. Biwer, Esilida Sula Karreci, Sonako Jacas, Maxim Mastyugin, Marianna Török, Brett C. Young, Prakash Jagtap, Garry J. Southan, and et al. 2023. "A Novel Dual-Function Nitric Oxide Donor Therapy for Preeclampsia—A Proof-of-Principle Study in a Murine Model" Antioxidants 12, no. 12: 2036. https://0-doi-org.brum.beds.ac.uk/10.3390/antiox12122036