Development and Validation of Conditions for Extracting Flavonoids Content and Evaluation of Antioxidant and Cytoprotective Activities from Bougainvillea x buttiana Bracteas (var. Rose)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals Reagents
2.2. Preparation of Bougainvillea x buttiana Extract
2.3. Determination of Flavonoids
2.3.1. Samples Preparation
2.3.2. Standard Solution Preparation
2.3.3. Determination of Flavonoids by High Performance Liquid Chromatography (HPLC)
2.3.4. Calibration Curve
2.4. Estimation of Total Flavonoid Content (TFC)
2.4.1. Experimental Schedule
2.4.2. In Vitro Antioxidant Activity by DPPH Method
2.4.3. Antioxidant Activity Index (AAI)
2.5. Cell Proliferation and Viability Assay Using L929 Fibroblast
Hydrogen Peroxide-Induced Oxidative Stress in L929
2.6. Statistics
3. Results
3.1. Phytocompound Analyses
3.2. Total Flavonoids Contents
3.3. Determination of Flavonoids by HPLC
3.4. Determination Antioxidant Activity
3.5. IC50 Value of DPPH Radical Scavenging Activity and Activity Antioxidant Index
3.6. Ethanol Concentration Effect on the Cellular Viability
3.7. Ethanol Concentration Effect on H2O2 Induced Oxidative Stress and Cell Survival
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Experiments | Treatment |
---|---|
1 | Cells were treated for 24 h followed by 1.0 mM of H2O2 exposure for 3 h |
2 | Cells were exposed concomitantly to each extract and 1.0 mM of H2O2 for 24 h |
3 | Cells were exposed to 1.0 mM of H2O2 for 3 h followed by cells treatment with each extract for 24 h. Evaluation of cell survival was performed using MTT assay as described above. |
Name of Compound | Structure |
---|---|
Saturated fatty acids | |
n-Hexadecanoic acid | |
Octadecanoic acid | |
Polyunsaturated fatty acids | |
9-Octadecenoic acid (E)- | |
9,12-Octadecadienoic acid (Z,Z)- | |
Esterified fatty acids | |
9,12-Octadecadienoic acid, ethyl ester | |
Hexadecanoic acid, methyl ester | |
Hexadecanoic acid, ethyl ester | |
Phenolic compounds | |
2-Propenoic acid, 3-(2-hydroxyphenyl)-, (E)- | |
2-Methoxy-4-vinylphenol | |
Ethanone, 1-(2-hydroxy-5-methylphenyl)- | |
Volatile compounds | |
Naphthalene, 3,4-dihydro-1,8-bis(trimethylsilyloxy)- | |
Benzofuran, 2,3-dihydro- | |
2,5-Dimethyl-4-hydroxy-3-(2H)-furanone | |
4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl | |
Sterols | |
Stigmasta-5,22-dien-3-ol | |
Carbohidrates | |
3-O-Methyl-d-glucose |
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Abarca-Vargas, R.; Zamilpa, A.; Petricevich, V.L. Development and Validation of Conditions for Extracting Flavonoids Content and Evaluation of Antioxidant and Cytoprotective Activities from Bougainvillea x buttiana Bracteas (var. Rose). Antioxidants 2019, 8, 264. https://0-doi-org.brum.beds.ac.uk/10.3390/antiox8080264
Abarca-Vargas R, Zamilpa A, Petricevich VL. Development and Validation of Conditions for Extracting Flavonoids Content and Evaluation of Antioxidant and Cytoprotective Activities from Bougainvillea x buttiana Bracteas (var. Rose). Antioxidants. 2019; 8(8):264. https://0-doi-org.brum.beds.ac.uk/10.3390/antiox8080264
Chicago/Turabian StyleAbarca-Vargas, Rodolfo, Alejandro Zamilpa, and Vera L. Petricevich. 2019. "Development and Validation of Conditions for Extracting Flavonoids Content and Evaluation of Antioxidant and Cytoprotective Activities from Bougainvillea x buttiana Bracteas (var. Rose)" Antioxidants 8, no. 8: 264. https://0-doi-org.brum.beds.ac.uk/10.3390/antiox8080264