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Article

A Cloned Recombinant Vesicular Stomatitis Virus-Vectored Marburg Vaccine, PHV01, Protects Guinea Pigs from Lethal Marburg Virus Disease

1
Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3E 3R2, Canada
2
Public Health Vaccines, Cambridge, MA 02142, USA
*
Author to whom correspondence should be addressed.
Current Address: National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada.
Current Address: World Health Organization, 1211 Geneva, Switzerland.
§
Current Address: Boston Children’s Hospital, Harvard Medical School, Cambridge, MA 02115, USA.
Academic Editor: François Meurens
Received: 20 May 2022 / Revised: 10 June 2022 / Accepted: 12 June 2022 / Published: 23 June 2022
Marburg virus (MARV) is a negative-sense, single-stranded RNA virus that belongs to the Filoviridae family. Despite having caused numerous outbreaks of severe hemorrhagic fever with high case fatality rates, there are still no clinically approved therapeutics or vaccines to treat or prevent MARV disease. Recombinant vesicular stomatitis viruses (rVSVs) expressing heterologous viral glycoproteins have shown remarkable promise as live-attenuated vaccine vectors, with an rVSV-based Ebola virus vaccine having received regulatory approval in the United States and numerous other countries. Analogous rVSV vaccine vectors have also been developed for MARV and have shown efficacy in several preclinical studies conducted in nonhuman primates. Here, we used a guinea pig model to confirm the protective efficacy of a cloned, rVSV-based candidate vaccine, termed PHV01, expressing the MARV variant Angola glycoprotein. Our results demonstrated that a single dose (2 × 106 PFU) of vaccine administered 28 days prior to challenge with a uniformly lethal dose of guinea-pig-adapted MARV variant Angola provided complete protection from death and disease. Moreover, protection was robust, with as little as 200 PFU of vaccine conferring significant protection. Not only does this study highlight the potential predictive value of the guinea pig model in the evaluation of MARV countermeasures, but it also demonstrates consistent and reproducible protection afforded by a clonal vaccine candidate. Indeed, this study identifies PHV01 as a suitable vaccine candidate for advanced development. View Full-Text
Keywords: Marburg virus; VSV-MARV; vesicular stomatitis virus; vaccine; filovirus Marburg virus; VSV-MARV; vesicular stomatitis virus; vaccine; filovirus
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MDPI and ACS Style

Zhu, W.; Liu, G.; Cao, W.; He, S.; Leung, A.; Ströher, U.; Fairchild, M.J.; Nichols, R.; Crowell, J.; Fusco, J.; Banadyga, L. A Cloned Recombinant Vesicular Stomatitis Virus-Vectored Marburg Vaccine, PHV01, Protects Guinea Pigs from Lethal Marburg Virus Disease. Vaccines 2022, 10, 1004. https://0-doi-org.brum.beds.ac.uk/10.3390/vaccines10071004

AMA Style

Zhu W, Liu G, Cao W, He S, Leung A, Ströher U, Fairchild MJ, Nichols R, Crowell J, Fusco J, Banadyga L. A Cloned Recombinant Vesicular Stomatitis Virus-Vectored Marburg Vaccine, PHV01, Protects Guinea Pigs from Lethal Marburg Virus Disease. Vaccines. 2022; 10(7):1004. https://0-doi-org.brum.beds.ac.uk/10.3390/vaccines10071004

Chicago/Turabian Style

Zhu, Wenjun, Guodong Liu, Wenguang Cao, Shihua He, Anders Leung, Ute Ströher, Michael J. Fairchild, Rick Nichols, Joseph Crowell, Joan Fusco, and Logan Banadyga. 2022. "A Cloned Recombinant Vesicular Stomatitis Virus-Vectored Marburg Vaccine, PHV01, Protects Guinea Pigs from Lethal Marburg Virus Disease" Vaccines 10, no. 7: 1004. https://0-doi-org.brum.beds.ac.uk/10.3390/vaccines10071004

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