Nutritional Composition, Bioactive Compounds and Functional Evaluation of Various Parts of Cajanus cajan (L.) Millsp
, Chen-I Chen 4 and Tuzz-Ying Song 2,*Round 1
Reviewer 1 Report
Manuscript ID: agriculture-979859
Type of manuscript: Article
Title: Nutritional composition, bioactive compounds and functional evaluation of various parts of Cajanus cajan (L.) Millsp
Comments and Suggestions for Authors
This study reported the basic nutritional composition and biological functions in leaves, seeds and roots of Cajanus cajan (L.) Millsp were evaluated. Three different extracting mixture were select for evaluation of the antioxidant, hypoglycemic and antibacterial activities, with a view to propose C. cajan (L.) Millsp extract as health product. Find my comments below:
Affiliations:
-line 6-11; review this part adding the missing information (such as abbreviations of names)
Introduction: The introduction section needs to be expanded, and the aim must be clearly stated in the last part of this section. Cajanus cajan (L.) could be reported in full name only the first time and then in their abbreviation form C. cajan (L.).
-line 35-37; remove the bold from the font.
Materials and methods: The methods must be explained in a clear and detailed manner in order to facilitate repeatability by other researchers.
Please add the chemicals and reagents paragraph in the section materials and methods.
-line 61; report the name (S. aureus, E. coli, P. gingivalis and S. mutans) in full and also in italic;
-line 61-68; detail the activation of bacterial species;
-line 72-73; report the weight of powder sample used in the extraction process;
-line 121; change 2M NaOH as NaOH 2M;
-line 124; What does the blank sample consist of?
-line 132; How was the IC50 calculated?
-line 139-141; again explain in detail the determination of the MIC.
Results:
-Table 1. Crude fat (g) for leaves (0.00) change as ND. In addition, divide the micronutrients from the macronutrients in the table by placing a line above.
Discussion:
-line 268, 271, 285, 288, 291, 298; remove the year in the bracket; follow the instruction for authors (https://0-www-mdpi-com.brum.beds.ac.uk/journal/agriculture/instructions);
Conclusions could be ameliorated.
References: should be checked according to the journal guidelines:
https://0-www-mdpi-com.brum.beds.ac.uk/journal/agriculture/instructions
Author Response
Reply to the reviewer-1 comments
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Reviewer Number |
Original comments of the reviewer |
Reply by the author(s) |
Changes done on page number and line number |
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1 |
This study reported the basic nutritional composition and biological functions in leaves, seeds and roots of Cajanus cajan (L.) Millsp were evaluated. Three different extracting mixture were select for evaluation of the antioxidant, hypoglycemic and antibacterial activities, with a view to propose C. cajan (L.) Millsp extract as health product. Find my comments below: Affiliations: -line 6-11; review this part adding the (such as abbreviations of names) |
We now have added the missing information in MS.
|
We add an author (Chen-I Chen) who provides C. cajan samples.
Page 1, line 7-11, highlighted in red. |
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2 |
Introduction: The introduction section needs to be expanded, and the aim must be clearly stated in the last part of this section. Cajanus cajan (L.) could be reported in full name only the first time and then in their abbreviation form C. cajan (L.). -line 35-37; remove the bold from the font. |
1. We have expanded the introduction section and corrected the abbreviation of C. cajan.
2. We have removed the bold from the font. |
Page 2, line 57-59, highlighted in red.
Page 2, line 40-42. |
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3 |
Materials and methods: The methods must be explained in a clear and detailed manner in order to facilitate repeatability by other researchers. Please add the chemicals and reagents paragraph in the section materials and methods. -line 61; report the name (S. aureus, E. coli, P. gingivalis and S. mutans) in full and also in italic;
-line 61-68; detail the activation of bacterial species;
-line 72-73; report the weight of powder sample used in the extraction process;
-line 121; change 2M NaOH as NaOH 2M;
-line 124; What does the blank sample consist of?
-line 132; How was the IC50 calculated?
- line 139-141; again explain in detail the determination of the MIC . |
1. We have added Chemicals and Reagents paragraph in the section of materials and methods, and provide clear and detailed methods in MS.
2. We have corrected S. aureus, E. coli, P. gingivalis and S. mutans in full name and also in italic. 3. We have detailed the activation of bacterial species. 4. We added the powder weight of sample in MS.
5. We have change 2M NaOH to NaOH 2M.
6. We have added the composition of the solution contained in the blank group.
7. We have added the IC50 calculated methods.
8. We have explained the determination of the MIC in detail of the MS. |
Page 2, line 65-78, highlighted in red.
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Page 2, line 82-84, highlighted in red. Page 2, line 95-96, highlighted in red.
Page 4, line 170, highlighted in red.
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4 |
Results: -Table 1. Crude fat (g) for leaves (0.00) change as ND. In addition, divide the micronutrients from the macronutrients in the table by placing a line above.
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1. We have corrected these mistakes and divide the micronutrients from the macronutrients in the table by placing a line above. |
Page 5, line 210-211, highlighted in red. |
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5 |
Discussion: -line 268, 271, 285, 288, 291, 298; remove the year in the bracket; follow the instruction for authors (https://0-www-mdpi-com.brum.beds.ac.uk/journal/agriculture/instructions); Conclusions could be ameliorated.
References: should be checked according to the journal guidelines: https://0-www-mdpi-com.brum.beds.ac.uk/journal/agriculture/instructions |
1. We have removed the year in the bracket.
2. Conclusions have been ameliorated.
3. We have checked the references according to the journal guidelines.
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Page 11, line 266-355.
Page 12, line 403-409, highlighted in red.
Page 12-13, line 419-505, highlighted in red. |
Reviewer 2 Report
The subject of this study is of interest and correct introduced, and the discussion is detailed, well documented and interesting. However it is minor concerns about the presentation of the obtained data:
Abstract:
General: Passive sentences should be preferred
Materials and methods:
General remark relates to the unknown regarding the origin and cultivation of the analyzed sample and the inclusion of only one sample in the study. Some additional information needs to be listed: the geographical origin of the sample, the basic characteristics of the climate, the type of soil, etc., indicating the Institution which provided the sample.
Line 73- The powder was extracted in a ratio of 1:20 with hot water at a temperature of 98 ˚C for 3 h and with 50% and 90% ethanol by soaking at room temperature for 72 h.
Results:
Line 152-…of magnesium (138.8 and 130.0 mg/100g, respectively) and calcium (581 and 597 mg/100g, respectively)
Table 1:
Add statistical significance between L, S and R for listed parameters
Add as last row Total under L, S and R – as in Table 2
Table2:
Add statistical significance between L, S and R for listed parameters
Add Total of BCAA amino acids under L, S and R
Table 3:
In Yields column – subscript a is missing – significant difference
Line 217 – space - and α-amylase activities
Table 7. Move subscript 4 to 303±12d
Line 224 – space AGEs;3
Line 226 – space-(means±SD, n=3)
Discussion:
Add a few recent references to the research topic
Author Response
Reply to the reviewer-2 comments
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Reviewer Number |
Original comments of the reviewer |
Reply by the author(s) |
Changes done on page number and line number |
|
1 |
The subject of this study is of interest and correct introduced, and the discussion is detailed, well documented and interesting. However it is minor concerns about the presentation of the obtained data: Abstract: General: Passive sentences should be preferred
|
1. We changed the sentences of the abstract from active to passive voices. |
Page 1, line 23-25, highlighted in red. |
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2 |
Materials and methods: General remark relates to the unknown regarding the origin and cultivation of the analyzed sample and the inclusion of only one sample in the study. Some additional information needs to be listed: the geographical origin of the sample, the basic characteristics of the climate, the type of soil, etc., indicating the Institution which provided the sample. Line 73- The powder was extracted in a ratio of 1:20 with hot water at a temperature of 98 ˚C for 3 h and with 50% and 90% ethanol by soaking at room temperature for 72 h.
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1.We have added the resource information about C. cajan.
2. We have corrected this mistake as following: Dried roots, leaf and seeds of C. cajan (500 g) were extracted with 1:20 ratio (w/v) of 98±2 ℃ hot water (10 L)……… |
Page 3, line 91-93, highlighted in red.
Page 3, line 95-96, highlighted in red.
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3 |
Results: Line 152-…of magnesium (138.8 and 130.0 mg/100g, respectively) and calcium (581 and 597 mg/100g, respectively) Table 1: Add statistical significance between L, S and R for listed parameters Add as last row Total under L, S and R – as in Table 2 Table2: Add statistical significance between L, S and R for listed parameters Add Total of BCAA amino acids under L, S and R
Table 3: In Yields column – subscript a is missing – significant difference
Line 217 – space - and α-amylase activities
Table 7. Move subscript 4 to 303±12d
Line 224 – space AGEs;3
Line 226 – space-(means±SD, n=3)
|
1. We have added statistical significance in Table 1 and 2.
2. We have added BCAA amino acids content of roots, leaf and seeds of C. cajan and statistical significance in Table 2.
3. We have corrected this mistake.
4. We have deleted the space.
5. We have moved subscript 4 to 303±12d.
6. We have deleted the space.
7. We have deleted the space. |
Page 5-6, Table 1 and 2, highlighted in red.
Page 6, Table 2, highlighted in red.
Page 7, Table 3, highlighted in red.
Page 9, line 279.
Page 9, Table 3 (L283), highlighted in red.
Page 9, line 279.
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4 |
Discussion: Add a few recent references to the research topic |
1. We have added a few recent references to the Discussion. |
Page 13-14, Ref 29,40, highlighted in red. |
Round 2
Reviewer 1 Report
The requested changes have been made. It can be accepted for publication.
