Identification of the Citrus Superoxide Dismutase Family and Their Roles in Response to Phytohormones and Citrus Bacterial Canker
Round 1
Reviewer 1 Report
In this article, the authors conducted an insilico investigation to explore and characterise Superoxide dismutases (SODs) gene family in Citrus sinensis, and explored the expression patterns of these genes in response to citrus bacterial canker (CBC) infection. The article is well written and is important for breeding Citrus sinensis for resistance to citrus bacterial canker. I have found a few minor mistakes (dot in line 59). I also request the authors to check the typos, punctuation, and references (i.e. e24952 missing in line 498).
Author Response
Errors in line 59 have been corrected.
The reference 3 (line 498) were double checked and correct: Feng, K.; Yu, J.; Cheng, Y.; Ruan, M.; Wang, R.; Ye, Q.; Zhou, G.; Li, Z.; Yao, Z.; Yang, Y.; Zheng, Q.; Wan, H. The SOD Gene Family in Tomato: Identification, Phylogenetic Relationships, and Expression Patterns. Front Plant. Sci 2016, 7, 1279.
Reviewer 2 Report
1. The study examines the bioinformatics approach to analyze the SOD gene family and explore the expression of its members in response to Xcc infection and phyto hormones (methyl jasmonate, MeJA; abscisic acid, ABA; and salicylic acid, SA). The findings shed light on the understanding of the associations between SODs and CBC infection as well as identify suitable genes for molecular breeding of Citrus which is a popular fruit crop throughout the world.
Strengths: The study aims to use a bioinformatics approach to investigate the relationship between the regulation SOD gene family and explore the expression of its members in response to Xcc infection and phytohormones (methyl jasmonate, MeJA; abscisic acid, ABA; and salicylic acid, SA). The findings of the present study will extend our understanding of the associations between citrus SODs and CBC infection as well as identify suitable genes for molecular breeding.
Introduction Limitations: The research conducted by Wen Yang et al. is commendable, however, the introduction part of the article needs to be cited with the latest citations as the author has cited one 2021 article and the rest of the citations are too old given the scope of the study.
Materials and methods: The design is appropriate, and the results are validated with the databases (physiochemical predictions, secondary structure predictions, Chromosomal distribution of CsSOD genes, by MAPCHART V2.1, gene structures of CsSODs visualization with 222 GSDS V2.0, Pfam analysis, Phylogenetic and collinearity analyses.
Results: These are well presented in Figures and Tables format and are in order.
Conclusion: There is scope for improvement. Authors need to highlight what is the future of their research and its implications globally.
References: The references are according to the journal's style.
Overall: The design of the research is appropriate ● The study has the potential to add a good piece of knowledge to the scientific community to Citrus research ● The language is appropriate.
Author Response
The study examines the bioinformatics approach to analyze the SOD gene family and explore the expression of its members in response to Xcc infection and phyto hormones (methyl jasmonate, MeJA; abscisic acid, ABA; and salicylic acid, SA). The findings shed light on the understanding of the associations between SODs and CBC infection as well as identify suitable genes for molecular breeding of Citrus which is a popular fruit crop throughout the world.
Strengths: The study aims to use a bioinformatics approach to investigate the relationship between the regulation SOD gene family and explore the expression of its members in response to Xcc infection and phytohormones (methyl jasmonate, MeJA; abscisic acid, ABA; and salicylic acid, SA). The findings of the present study will extend our understanding of the associations between citrus SODs and CBC infection as well as identify suitable genes for molecular breeding.
Introduction Limitations: The research conducted by Wen Yang et al. is commendable, however, the introduction part of the article needs to be cited with the latest citations as the author has cited one 2021 article and the rest of the citations are too old given the scope of the study.
AUTHOR RESPONSE：
According to the reviewer’s suggestion, we cited some new articles (Ref 5, 7, 8, 9, 14, 17, 29).
Materials and methods: The desig n is appropriate, and the results are validated with the databases (physiochemical predictions, secondary structure predictions, Chromosomal distribution of CsSOD genes, by MAPCHART V2.1, gene structures of CsSODs visualization with 222 GSDS V2.0, Pfam analysis, Phylogenetic and collinearity analyses.
Results: These are well presented in Figures and Tables format and are in order.
Conclusion: There is scope for improvement. Authors need to highlight what is the future of their research and its implications globally.
AUTHOR RESPONSE：
We reworded the conclusion part according to the reviewers’ suggestion. (Conclusion section)
References: The references are according to the journal's style.
Overall: The design of the research is appropriate ● The study has the potential to add a good piece of knowledge to the scientific community to Citrus research ● The language is appropriate.
