The Occurrence of Oxidative Stress Induced by Silver Nanoparticles in Chlorella vulgaris Depends on the Surface-Stabilizing Agent
Abstract
:1. Introduction
2. Materials and Methods
2.1. AgNP Synthesis and Characterization
2.2. Chlorella vulgaris Cell Culture
2.3. Cell Viability Assay by Flow Cytometry
2.4. AgNP Stability in Liquid BBM Medium
2.5. Silver Content Measurements
2.6. Protein Extraction
2.7. Reactive Oxygen Species Determination
2.8. Malondialdehyde and Protein Carbonyl Content
2.9. Comet Assay
2.10. Activity Assays of Antioxidant Enzymes
2.11. Assays of Non-Enzymatic Antioxidants
2.12. Ultrastructural Analyses
2.13. Statistical Analysis
3. Results
3.1. AgNP Characterization
3.2. Cell Viability Assay by Flow Cytometry
3.3. AgNP Stability in Liquid BBM Medium
3.4. Silver Content Measurements
3.5. Induction of ROS Formation
3.6. Oxidative Effect of AgNP on Lipids, Proteins and DNA
3.7. Antioxidant Enzymes Activity
3.8. Non-Enzymatic Antioxidants
3.9. Ultrastructure Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatment | µg 10−6 Cells |
---|---|
Control | 0 d |
AgNP-citrate | 15.62 a |
AgNP-CTAB | 26.81 b |
AgNO3 | 9.96 c |
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Komazec, B.; Cvjetko, P.; Balen, B.; Letofsky-Papst, I.; Lyons, D.M.; Peharec Štefanić, P. The Occurrence of Oxidative Stress Induced by Silver Nanoparticles in Chlorella vulgaris Depends on the Surface-Stabilizing Agent. Nanomaterials 2023, 13, 1967. https://0-doi-org.brum.beds.ac.uk/10.3390/nano13131967
Komazec B, Cvjetko P, Balen B, Letofsky-Papst I, Lyons DM, Peharec Štefanić P. The Occurrence of Oxidative Stress Induced by Silver Nanoparticles in Chlorella vulgaris Depends on the Surface-Stabilizing Agent. Nanomaterials. 2023; 13(13):1967. https://0-doi-org.brum.beds.ac.uk/10.3390/nano13131967
Chicago/Turabian StyleKomazec, Bruno, Petra Cvjetko, Biljana Balen, Ilse Letofsky-Papst, Daniel Mark Lyons, and Petra Peharec Štefanić. 2023. "The Occurrence of Oxidative Stress Induced by Silver Nanoparticles in Chlorella vulgaris Depends on the Surface-Stabilizing Agent" Nanomaterials 13, no. 13: 1967. https://0-doi-org.brum.beds.ac.uk/10.3390/nano13131967