Recent Advances in Multicellular Tumor Spheroid Generation for Drug Screening
Abstract
:1. Introduction
2. 3D Cell Culture Technology
2.1. Anchorage-Independent Approaches
2.1.1. Hanging-Drop Method
2.1.2. Rotary Cell Culture System
2.2. Anchorage-Independent Approaches
2.2.1. Porous Scaffold
2.2.2. Fibrous Scaffold
2.2.3. Gel-Based Scaffolds
3. Multicellular Cancer Spheroid Formation Platform
3.1. Hydrogel-Based Platforms
3.2. Microarray-Based Platform
3.3. Matrix-Based Platform
4. Biosensing Methods to Assess Drug Efficacy in Multicellular Spheroids
4.1. Invasive Sensing Methods
4.1.1. Immunofluorescence
4.1.2. Cell Viability Assay
4.2. Non-Invasive Sensing Methods
4.2.1. Electrochemical Biosensing
4.2.2. Optical Coherence Tomography-Based Biosensing
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Cell Line | Culture Method | Substrate Type | Ref. |
---|---|---|---|
MCF-7, MDA-MB231 | Hanging drop | - | [28] |
BT474 | Hanging drop | - | [29] |
MCF-7 | Hanging drop | Poly(N-isopropylacrylamide) (p(NIPA)) | [30] |
MDA-MB-231 | Rotary cell culture system | - | [31] |
HGC-27 | Rotary cell culture system | - | [32] |
HeLa, MCF-7, HUVECs | Porous scaffold | PDMS/CMC/PEDOT/Pt composites | [33] |
PC3 | Porous scaffold | Cellulose nanocrystals/poly(oligoethylene glycol methacrylate) | [34] |
DU 145, A549, MCF-7, MDA-MB-231 | Fibrous scaffold | Core–shell silk fibroin/rice paper | [35] |
LNCaP | Gel-based scaffold | bQ13 (Ac-QQKFQFQFEQEQQ-Am) peptide | [36] |
MCF-7, A549, A2780, P19, Panc02, UN-KC-6141 | Hydrogel-based platform | Poly dimethyl siloxane (PDMS) derivatives (acoustic devices) | [37] |
MCF-7 | Hydrogel-based platform | ClO−/SCN−/carboxymethyl cellulose | [38] |
NIH3T3, HepG2, HUVECs | Hydrogel-based platform | PEG-SH/Gela-SH/Gly-Tyr/D-PBS/HRP | [39] |
A549, T24, Huh-7 | Microwell-based platform | Polystyrene slides/PDMS | [40] |
A549, MG-63, HLFs | Microwell-based hydrogel platform | N-isopropylacrylamide (NIPAM) | [41] |
HepG2, HEK 293T | Microarray-based platform | Droplet microarray slides (DMA) | [42] |
HUVEC, MCF-7 | Matrix-based platform | Poly dimethyl siloxane (PDMS)/collagen | [43] |
PANC-1, PS-1, HMEC | Matrix-based platform | Polyurethane/fibronectin (FN)/collagen I (COL) | [44] |
Cell Line | Anticancer Drug | Screening Tools | Ref. |
---|---|---|---|
MCF-7, HeLa, Caco-2 | 5-fluorouracil, cetuximab, panitumumab | Immunofluorescence | [115] |
HT-29 | Perifosine | Immunofluorescence | [116] |
HT-29 | Carboxyl-modified polystyrene nanoparticle | Immunofluorescence | [117] |
Human breast cancer cell line (MCF-7 and MDA-MB-231) | Copper(II)-tropolone complex | Cell viability assay | [118] |
MCF7, MDA-MB-231, SKBR3, MCF12A | Preussin | Cell viability assay | [119] |
MCF-7 | PLGA-MnO2 nanoparticles | Cell viability assay | [120] |
HCT-116 | Doxorubicin | Electrical impedance spectroscopy (EIS) | [121] |
Human neuroblastoma and glioblastoma (SH-SY5Y, U-87MG) | Curcumin | Electrochemical detection (Cyclic voltammetry, Differential Pulse Voltammetry) | [122] |
HCT-116 | Immersion media (glycerol and ScaleView-A2) | Optical coherence tomography | [123] |
MCF7 | Paclitaxel | Optical coherence tomography | [110] |
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Lee, K.-H.; Kim, T.-H. Recent Advances in Multicellular Tumor Spheroid Generation for Drug Screening. Biosensors 2021, 11, 445. https://0-doi-org.brum.beds.ac.uk/10.3390/bios11110445
Lee K-H, Kim T-H. Recent Advances in Multicellular Tumor Spheroid Generation for Drug Screening. Biosensors. 2021; 11(11):445. https://0-doi-org.brum.beds.ac.uk/10.3390/bios11110445
Chicago/Turabian StyleLee, Kwang-Ho, and Tae-Hyung Kim. 2021. "Recent Advances in Multicellular Tumor Spheroid Generation for Drug Screening" Biosensors 11, no. 11: 445. https://0-doi-org.brum.beds.ac.uk/10.3390/bios11110445