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Article

Genetic Profiles and Antimicrobial Resistance Patterns of Salmonella Infantis Strains Isolated in Italy in the Food Chain of Broiler Meat Production

1
Department of Veterinary Medicine, Via S. Costanzo 4, 06126 Perugia, Italy
2
Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche “Togo Rosati”, Via G. Salvemini, 1, 06126 Perugia, Italy
3
Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161 Roma, Italy
4
Department of Agricultural and Food Sciences, Viale Fanin 42, 40127 Bologna, Italy
*
Author to whom correspondence should be addressed.
Received: 30 October 2020 / Revised: 13 November 2020 / Accepted: 14 November 2020 / Published: 16 November 2020
(This article belongs to the Special Issue Antimicrobial-Resistance of Food-Borne Pathogens)
This work aimed to evaluate the antimicrobial susceptibility of 87 Salmonella Infantis strains isolated in Italy from 2016 to 2019 along the food chain of broiler meat production and in humans and to determine the genetic profiles of the strains in order to establish a possible correlation with the antimicrobial pattern. All isolates were tested by the disk diffusion method to evaluate antimicrobial susceptibility toward sixteen antimicrobials, and the broth microdilution method was used to confirm extended spectrum β-lactamase (ESBL) production. PCR and pulsed field gel electrophoresis (PFGE) were applied to characterize ESBL-encoding and AmpC β-lactamase genes and to analyze the S. Infantis strains genetic profiles respectively. S. Infantis isolates showed high prevalence of resistance, in particular toward nalidixic acid (97.7%), tetracycline (96.5%), sulphamethoxazole/trimethoprim (91%) and cefepime (72.4%). The 80.5% of isolates were ESBL, cefotaxime-resistant, carrying the blaCTX-M1 gene. The most prevalent PFGE profile was XbaI.0126 (35.6%). The remaining strains had a genetic homology from 81% to 97% with the XbaI.0126 profile. The strains belonging to these profiles were isolated from different matrices collected along the broiler food chain independently on the year and from the region and there was no correlation between the PFGE profiles and resistance patterns. We found two ESBL-producing S. Infantis strains with the same XbaI.2621 profile isolated from humans and from poultry feces, not yet reported in Italy. Our findings confirmed the diffusion of ESBL-multi drug resistant (MDR) S. Infantis along the broiler food chain and in humans and underlined the importance of continuous monitoring to control and to reduce the prevalence of this bacterium, applying a global One Health approach. View Full-Text
Keywords: Salmonella Infantis; antimicrobial susceptibility; ESBL; genetic profile; PFGE Salmonella Infantis; antimicrobial susceptibility; ESBL; genetic profile; PFGE
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MDPI and ACS Style

Proietti, P.C.; Stefanetti, V.; Musa, L.; Zicavo, A.; Dionisi, A.M.; Bellucci, S.; Mensa, A.L.; Menchetti, L.; Branciari, R.; Ortenzi, R.; Franciosini, M.P. Genetic Profiles and Antimicrobial Resistance Patterns of Salmonella Infantis Strains Isolated in Italy in the Food Chain of Broiler Meat Production. Antibiotics 2020, 9, 814. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9110814

AMA Style

Proietti PC, Stefanetti V, Musa L, Zicavo A, Dionisi AM, Bellucci S, Mensa AL, Menchetti L, Branciari R, Ortenzi R, Franciosini MP. Genetic Profiles and Antimicrobial Resistance Patterns of Salmonella Infantis Strains Isolated in Italy in the Food Chain of Broiler Meat Production. Antibiotics. 2020; 9(11):814. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9110814

Chicago/Turabian Style

Proietti, Patrizia C., Valentina Stefanetti, Laura Musa, Alessia Zicavo, Anna M. Dionisi, Sara Bellucci, Agnese L. Mensa, Laura Menchetti, Raffaella Branciari, Roberta Ortenzi, and Maria P. Franciosini 2020. "Genetic Profiles and Antimicrobial Resistance Patterns of Salmonella Infantis Strains Isolated in Italy in the Food Chain of Broiler Meat Production" Antibiotics 9, no. 11: 814. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics9110814

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