Citral-Enriched Fraction of Lemon Essential Oil Mitigates LPS-Induced Hepatocyte Injuries
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5 Diphenyl Tetrazolium Bromide) Assay
2.3. RNA Isolation and Quantitative Real-Time PCR
2.4. ELISA (Enzyme Immunosorbent Assay)
2.5. Western Blot
2.6. Confocal Microscopy
2.7. Dichlorodihydrofluorescein Diacetate (DCFH-DA) Assay
2.8. Statistical Analysis
3. Results
3.1. In Vitro Anti-Inflammatory Effects of Cfr-LEO
3.2. Antioxidant Effects of Cfr-LEO
3.3. Cfr-LEO Protects Hepatocytes from the LPS-Induced EMT
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primers. | Forward | Reverse |
---|---|---|
GAPDH | ATGGGGAAGGTGAAGGTCG | GGGTCATTGATGGCAACAATAT |
IL-6 | GGTACATCCTCGACGGCATCT | GTGCCTCTTTGCTGCTTTCAC |
IL-1β | ACAGATGAAGTGCTCCTTCCA | GTCGGAGATTCGTAGCTGGAT |
TNFα | CCAGGCAGTCAGATCATCTTCTCTC | AGCTGGTTATCTCTCAGCTCCAC |
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Gasparro, R.; Pucci, M.; Costanzo, E.; Urzì, O.; Tinnirello, V.; Moschetti, M.; Conigliaro, A.; Raimondo, S.; Corleone, V.; Fontana, S.; et al. Citral-Enriched Fraction of Lemon Essential Oil Mitigates LPS-Induced Hepatocyte Injuries. Biology 2023, 12, 1535. https://0-doi-org.brum.beds.ac.uk/10.3390/biology12121535
Gasparro R, Pucci M, Costanzo E, Urzì O, Tinnirello V, Moschetti M, Conigliaro A, Raimondo S, Corleone V, Fontana S, et al. Citral-Enriched Fraction of Lemon Essential Oil Mitigates LPS-Induced Hepatocyte Injuries. Biology. 2023; 12(12):1535. https://0-doi-org.brum.beds.ac.uk/10.3390/biology12121535
Chicago/Turabian StyleGasparro, Roberta, Marzia Pucci, Elisa Costanzo, Ornella Urzì, Vincenza Tinnirello, Marta Moschetti, Alice Conigliaro, Stefania Raimondo, Valeria Corleone, Simona Fontana, and et al. 2023. "Citral-Enriched Fraction of Lemon Essential Oil Mitigates LPS-Induced Hepatocyte Injuries" Biology 12, no. 12: 1535. https://0-doi-org.brum.beds.ac.uk/10.3390/biology12121535