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Open AccessArticle
Vibration Emissions Reduce Boar Sperm Quality via Disrupting Its Metabolism
by
Shanpeng Wang
Shanpeng Wang 1,†,
Xuejun Zeng
Xuejun Zeng 2,3,†,
Shenao Liu
Shenao Liu 1,
S. A. Masudul Hoque
S. A. Masudul Hoque 4
,
Lingjiang Min
Lingjiang Min 1,
Nengshui Ding
Nengshui Ding 2,3,5,* and
Zhendong Zhu
Zhendong Zhu 1,*
1
College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China
2
Fujian Aoxin Biotechnology Group Co., Ltd., Zhangzhou 363000, China
3
Ji’an Aobao Biotechnology Group Co., Ltd., Ji’an 343000, China
4
Department of Animal Breeding and Genetics, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh
5
State Key Laboratory for Pig Genetic Improvement and Production Technology, Jiangxi Agricultural University, Nanchang 330045, China
*
Authors to whom correspondence should be addressed.
†
The authors contributed equally to this work.
Submission received: 28 February 2024
/
Revised: 24 March 2024
/
Accepted: 22 May 2024
/
Published: 23 May 2024
Simple Summary
Boar sperm is a highly sensitive biological product, and its quality is susceptible to various environmental factors during transportation. Presently, vibration emissions were observed and documented during the transportation of artificial insemination doses. Vibrations, serving as an environmental stressor, harbor the potential to exert an influence on sperm quality. However, the precise mechanism underlying the detrimental effects of vibration emissions on sperm functionality remains elusive. In the present research, we discerned that vibration emissions impair the quality of boar sperm by modulating their metabolic processes, ultimately resulting in a diminished fertilization capability.
Abstract
Artificial insemination (AI) with liquid-preserved semen has recently become common in pig breeding. The semen doses are produced in a centralized manner at the boar stud and then subsequently distributed and transported to sow farms. However, vibration emissions during transportation by logistic vehicles may adversely affect the quality of boar sperm. Therefore, this study aimed to explore the impact of vibration-induced emissions on sperm quality and function under simulated transportation conditions. Each time, ejaculates from all 15 boars were collected and then pooled together to minimize individual variations, and the sample was split using an extender for dilution. Different rotational speeds (0 rpm, 80 rpm, 140 rpm, 200 rpm) were utilized to simulate varying intensities of vibration exposure using an orbital shaker, considering different transportation times (0 h, 3 h, and 6 h). Subsequently, evaluations were conducted regarding sperm motility, plasma membrane integrity, acrosome integrity, mitochondrial function, adenosine triphosphate (ATP) levels, mitochondrial reactive oxygen species (ROS) levels, pH, glycolytic pathway enzyme activities, and capacitation following exposure to vibration emissions. Both vibration time and intensity impact sperm motility, plasma membrane integrity, and acrosomal integrity. Vibration exposure significantly reduced sperm ATP levels, mitochondrial membrane potential, and the levels of mitochondria-encoded proteins (MT-ND1, MT-ND6) (p < 0.05). After vibration emission treatment, the pH value and mitochondrial ROS levels significantly increased (p < 0.05). Inhibition of sperm glycolysis was observed, with reduced activities of hexokinase (HK), pyruvate kinase (PK), and lactate dehydrogenase (LDH), along with decreased lactate levels (p < 0.05). Additionally, sperm tyrosine phosphorylation levels were significantly reduced by vibration emissions compared to the control group (p < 0.05). After the vibration emission treatment, the number of sperm bound to each square millimeter of oviduct explants decreased significantly compared to the control group (p < 0.05). Similarly, compared to the control group, using semen subjected to vibration stress for AI results in significantly reduced pregnancy rates, total born litter size, live-born litter size, and healthy born litter size (p < 0.05).
Share and Cite
MDPI and ACS Style
Wang, S.; Zeng, X.; Liu, S.; Hoque, S.A.M.; Min, L.; Ding, N.; Zhu, Z.
Vibration Emissions Reduce Boar Sperm Quality via Disrupting Its Metabolism. Biology 2024, 13, 370.
https://0-doi-org.brum.beds.ac.uk/10.3390/biology13060370
AMA Style
Wang S, Zeng X, Liu S, Hoque SAM, Min L, Ding N, Zhu Z.
Vibration Emissions Reduce Boar Sperm Quality via Disrupting Its Metabolism. Biology. 2024; 13(6):370.
https://0-doi-org.brum.beds.ac.uk/10.3390/biology13060370
Chicago/Turabian Style
Wang, Shanpeng, Xuejun Zeng, Shenao Liu, S. A. Masudul Hoque, Lingjiang Min, Nengshui Ding, and Zhendong Zhu.
2024. "Vibration Emissions Reduce Boar Sperm Quality via Disrupting Its Metabolism" Biology 13, no. 6: 370.
https://0-doi-org.brum.beds.ac.uk/10.3390/biology13060370
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