In Vitro Models for Anti-Aging Efficacy Assessment: A Critical Update in Dermocosmetic Research
, Margarida C. Gonçalves 1,†, Matilde S. Marques 1,†, Francisco Veiga 1,2, Ana Cláudia Paiva-Santos 1,2,* and Patrícia C. Pires 1,2,3,*
Round 1
Reviewer 1 Report
The subject is suitable for publication. It’s a important and original contribution. The title clearly reflects the content. The keywords and abstract are sufficiently informative. The abstract presents clarity. The methodology is scientifically acceptable and is sufficiently detailed. The language and format are adequate. The literature is appropriate. All illustrations and tables are needed. The figures are very good and self-explanatory. The references are appropriate and current. The presentation, organization and size of the article are satisfactory. The introduction makes a coherent review of the topic addressed and the literature review corresponds to the proposed topic. And the method employed is appropriate to the type of study proposed.
Author Response
We thank the reviewer for their insightful and kind comments.
Reviewer 2 Report
Ms-Cosmetics-235625-review can be accepted.
The Manuscript is well set up and presents exhaustively the collections of the most recent studies on the molecules and techniques used to prevent skin aging. The studies analyzed by the authors, in vitro, both on cells and on engineered tissues is complete and useful as a source of information for researchers engaged in the field.
The introductory part is well set out and supported by a more recent bibliography. The interspersed use of graphical representations and schematic figures helps the reader maintain the point of interest. Since the drafting is easy to understand, it can certainly make an important contribution in the search for molecules of natural origin in particular, to be used not only in slowing down aging but also in preventing skin damage induced by acute and chronic inflammatory states deriving from exposure to molecules harmful to skin health.
The text must be double-checked for the presence of any typos.
The text must be double-checked for the presence of any typos.
Author Response
We thank the reviewer for their wise and thoughtful comments. The text has been double-checked for the presence of any typos and corrected accordingly (marked in blue).
Reviewer 3 Report
Suitable for publication
Authors have to report that animal assays have been banned for cosmetic products and cosmetic ingredients for cosmetic claims (SCCS). Only for specific reasons are allowed. (toxicity etc)
Regarding the paragraph 2.8 and TEWL
The experiments described by the authors have to do with moisturizing properties of cosmetic ingredients.
Moisturizing properties are not always a result of TEWL decrease.
Author Response
We thank the reviewer for their comments. All suggestions and corrections have been taken into account (changes marked in blue in the revised version of the manuscript), and a point-by-point answer in given below.
"Authors have to report that animal assays have been banned for cosmetic products and cosmetic ingredients for cosmetic claims (SCCS). Only for specific reasons are allowed. (toxicity etc)"
R: We thank the reviewer for their suggestion, which is indeed quite pertinent. A statement of such has been added from lines 87 to 89, along with the appropriate references.
"Regarding the paragraph 2.8 and TEWL
The experiments described by the authors have to do with moisturizing properties of cosmetic ingredients.
Moisturizing properties are not always a result of TEWL decrease."
R: We thank the reviewer for their comment. In fact, we agree that a decrease in moisturizing properties are not always a result of TEWL decrease, but it has been reported that it can be directly related. Hence, a clarification has been added regarding this issue, from lines 708 to 711.
