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Review

Strained Conformations of Nucleosides in Active Sites of Nucleoside Phosphorylases

by
Irina A. Il’icheva
†,
Konstantin M. Polyakov
† and
Sergey N. Mikhailov
*
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Str. 32, 119991 Moscow, Russia
*
Author to whom correspondence should be addressed.
†
Both authors contributed equally.
Biomolecules 2020, 10(4), 552; https://0-doi-org.brum.beds.ac.uk/10.3390/biom10040552
Submission received: 28 February 2020 / Revised: 26 March 2020 / Accepted: 1 April 2020 / Published: 5 April 2020
(This article belongs to the Special Issue Structural and Mechanistic Enzymology in Drug Discovery: A New Frontier)
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Abstract

:
Nucleoside phosphorylases catalyze the reversible phosphorolysis of nucleosides to heterocyclic bases, giving α-d-ribose-1-phosphate or α-d-2-deoxyribose-1-phosphate. These enzymes are involved in salvage pathways of nucleoside biosynthesis. The level of these enzymes is often elevated in tumors, which can be used as a marker for cancer diagnosis. This review presents the analysis of conformations of nucleosides and their analogues in complexes with nucleoside phosphorylases of the first (NP-1) family, which includes hexameric and trimeric purine nucleoside phosphorylases (EC 2.4.2.1), hexameric and trimeric 5′-deoxy-5′-methylthioadenosine phosphorylases (EC 2.4.2.28), and uridine phosphorylases (EC 2.4.2.3). Nucleosides adopt similar conformations in complexes, with these conformations being significantly different from those of free nucleosides. In complexes, pentofuranose rings of all nucleosides are at the W region of the pseudorotation cycle that corresponds to the energy barrier to the N↔S interconversion. In most of the complexes, the orientation of the bases with respect to the ribose is in the high-syn region in the immediate vicinity of the barrier to syn ↔ anti transitions. Such conformations of nucleosides in complexes are unfavorable when compared to free nucleosides and they are stabilized by interactions with the enzyme. The sulfate (or phosphate) ion in the active site of the complexes influences the conformation of the furanose ring. The binding of nucleosides in strained conformations is a characteristic feature of the enzyme–substrate complex formation for this enzyme group.

1. Introduction

1.1. Nucleoside Phosphorylases as Key Enzymes in Nucleoside Metabolism

Nucleoside phosphorylases (NPs) catalyze the phosphorolysis of the N-glycosidic bond in purine or pyrimidine β-d-ribo-(2’-deoxyribo)nucleosides and are found in almost all organisms. This class of enzymes includes purine nucleoside phosphorylases (PNPs; EC 2.4.2.1), 5′-deoxy-5′-methylthioadenosine phosphorylases (MTAPs; EC 2.4.2.28), uridine phosphorylases (UPs; EC 2.4.2.3), pyrimidine nucleoside phosphorylases (PyNPs; EC 2.4.2.2), and thymidine phosphorylases (TPs; EC 2.4.2.4). Scheme 1 depicts the general scheme of phosphorolysis of nucleosides by NPs. The equilibrium of the reaction is shifted towards nucleosides, the shift being much more significant for PNPs as compared with UPs. Uridine phosphorylases catalyze phosphorolysis of both thymidine and uridine [1]. Purine nucleoside phosphorylases cleave the N-glycosidic bond in purine ribonucleosides and 2’-deoxynucleosides.
Reactions that are catalyzed by nucleoside phosphorylases play an important role in maintaining nucleoside homeostasis in the body, the disruption of which leads to various diseases. The essential role of these enzymes in metathesis reactions in the organisms has stimulated interest in their exploration. Numerous reviews on nucleoside phosphorylases are available. The structural organization of these enzymes [1,2], the substrate specificity and methods for the synthesis of valuable nucleosides [3,4,5,6], and metabolism in biological systems [7,8,9,10] have been considered.
The biosynthesis of nitrogenous bases and nucleosides in most of organisms can proceed by two pathways. De novo synthesis utilizes some amino acids and small organic molecules as precursors. This biochemical pathway uses numerous enzymes and it has a high requirement for energy. The steps of the de novo synthesis are virtually the same in all organisms. An alternative salvage pathway, which repeatedly utilizes fragments of RNA, is energy saving and it varies in different organisms. In all organisms, reactions catalyzed by nucleoside phosphorylases play the key role in the salvage pathway. The regulation of nucleotide metabolism and biosynthesis are considered in relation to Escherichia coli and Salmonella enterica in [9] and in [10] for mammalian.
Purinergic signaling modulates fundamental pathophysiological processes, such as tissue homeostasis, wound healing, neurodegeneration, immunity, inflammation, and cancer [11]. Many cancer processes in humans are accompanied by the disturbance of pyrimidine metabolism. Colon carcinoma cells [12], melanoma tumors [13], breast adenocarcinoma cells [14], ascites hepatoma, and Ehrlich ascites carcinoma cells [15] have increased levels of uridine phosphorylase activity. Recent experiments in mice [16] showed that the disruption of uridine homeostasis causes cancer diseases. Nucleoside phosphorylase inhibitors provide the potential to correct metabolic defects in humans [8].
These inhibitors can also be applied in the therapy of human parasitic diseases. This area of application is based on the fact that most of parasites are not able to synthesize purines and pyrimidines de novo because their genomes lack genes encoding necessary enzymes. Hence, significant differences between parasite and human nucleoside phosphorylases provide the basis for the selective inhibition of parasite enzymes. 5’-Methylthioimmucillin-H was developed as a specific inhibitor for Plasmodium falciparum PNP and it was proposed for the treatment of malaria [17,18]. The helminth parasite Schistosoma mansoni also cannot synthesize purines de novo [19]. Hence, the search for inhibitors that are specific for Schistosoma mansoni PNP is a challenging problem. Another important problem is the search for inhibitors specific for Giardia lamblia UP, because this helminth parasite is unable to synthesize pyrimidines de novo [20].
X-Ray crystallography is one of the most commonly used techniques for studying the structure-function relation of enzymes. In 1990, the first three-dimensional structure of human erythrocytic PNP (hPNP) was determined at 3.2 Å resolution in complex with the substrate analogue 5′-iodoformycin B [21]. The enzyme molecule was found to be composed of three subunits that were related by a crystallographic threefold axis. The substrate analogue is bound in the active site at the interface between two subunits of the trimer. The substrate binding involves seven residues of one subunit and the Phe residue of the adjacent subunit. In 1995, the X-ray structure of E. coli UP was determined at 2.5 Å resolution [22]. The E. coli UP structure is a hexamer comprised three dimers that were related by a threefold axis. Each dimer is composed of two subunits related by a non-crystallographic twofold axis. The active sites of E. coli UP are formed by residues belonging to two adjacent subunits of the dimer. The structure of hexameric E. coli. PNP was established at 2.0 Å resolution in 1997 [23]. The active site of E. coli. PNP also involves residues of two adjacent subunits of the dimer.
All nucleoside phosphorylases were classified into two families based on the sequence homology analysis of NPs and a comparison of their three-dimensional structures—NP-I and NP-II [1]. The NP-I family comprises hexameric and trimeric PNPs (including the MTAP subfamily) and hexameric UPs. Hexameric PNPs are enzymes from lower organisms, such as bacteria and some eukaryotic protozoa, in particular, intracellular parasites. Generally, these enzymes have a very broad spectrum of substrate specificity and are active towards both 6-amino and 6-keto derivatives of purine nucleosides [1]. Hexameric MTAPs were only isolated from the thermostable archaea Sulfolobus solfataricus [24], Pyrococcus furiosus, and Aeropyrum pernix. 5-Methylthioadenosine, adenosine, its 2-chloro and 2-amino derivatives, and also 6-keto derivatives of purine nucleosides (guanine and inosine) are substrates for the former two hexameric MTAPs [1]. 5′-Deoxy-5′-methylthioadenosine phosphorylase that is isolated from Aeropyrum pernix has some unique characteristics. On one hand, it is active towards some pyrimidines, including cytidine and deoxycytidine, and, on the other hand, unlike most of the reported MTAPs, it phosphorolyzes 2′-fluoro-modified arabinoside [25]. Trimeric PNPs and MTAPs are found in higher organisms and bacteria. Trimeric PNPs are specific for 6-keto derivatives of purine nucleosides (guanosine and inosine, but not for xanthosine), and MTA is the main substrate for trimeric MTAPs [1]. Uridine phosphorylases from bacteria and higher organisms are generally hexamers [1]. However, Schistosoma mansoni UP is an exception [26]. This enzyme acts as a dimer. Uridine, deoxyuridine, and thymidine are substrates for UPs [1,6,27]. Meanwhile, cytidine can also serves as a substrate for S. cerevisiae UP [28].
The NP-II family includes PyNPs and TPs [1]. Thymidine phosphorylase has the highest specificity among the NPs. It is only active towards thymidine and 2’-deoxyuridine. The enzymes of the NP-II family have a dimeric quaternary structure. Monomers of nucleoside phosphorylases of the NP-II family are composed of two domains, which undergo significant rearrangements during the enzymatic reaction. High-resolution X-ray diffraction data are not available for complexes of nucleosides with nucleoside phosphorylases of the NP-II family.
This review presents an analysis of conformations of nucleosides and their analogues in complexes with nucleoside phosphorylases. Only the structures determined at 2 Å resolution or higher are considered, because atomic coordinates that are derived from lower-resolution X-ray data are not reasonably accurate to establish nucleoside conformations with certainty. The available X-ray diffraction data enable the conformational analysis of nucleosides in complexes with nucleoside phosphorylases of the NP-I family.

1.2. Structures of the Active Sites of Nucleoside Phosphorylases of the NP-I Family

The structures of monomers of all nucleoside phosphorylases that belong to the NP-I family can be superimposed based on the spatially equivalent secondary structure elements. Figure 1 shows the superposition of the three-dimensional structures of the monomers of hexameric PNP from B. cereus (in complex with adenosine and phosphate, PDB 3UAW, 1.20 Å [29]), trimeric PNP from Bos Taurus (in complex with immucilin H and phosphate, PDB 1B80, 1.5 Å [30]), and hexameric UP from Shewanella oneidensis (in complex with uridine and sulfate, PDB 4R2W, 1.6 Å, [31]).
The active sites of these three enzymes are well superimposed, as can be seen in Figure 1. Figure 2, Figure 3 and Figure 4 show the active sites of these enzymes (the orientation is the same as in Figure 1).
In all enzymes, the base of the nucleoside is bound in the hydrophobic pocket and it forms hydrogen bonds with the residues of the enzyme. In all cases, adjacent subunits of the oligomeric molecules are involved in the complex formation with nucleosides. In the figures, the adjacent subunits of the hexameric PNP and UP are colored in grey. For the trimeric PNP, the adjacent subunit is only involved in the formation of the hydrophobic pocket and it is not shown in Figure 4. The ribose moiety and phosphate (or sulfate) are bound in a similar fashion in the hexameric PNP and UP. This is due to the similar structures of the phosphate- or sulfate-binding subsites of these enzymes and the presence of a conserved glutamate residue that forms hydrogen bonds with the O2′ and O3′ atoms of the ribose moiety. The radically different binding of the ribose moiety and phosphate (or sulfate) is observed in the trimeric PNP.

1.3. Description of Nucleoside Conformations

The parameters P, θm, χ, ν2, and iTj that are given in Tables 1–3 adequately and fully characterize nucleoside conformations. The conformation of the pentofuranose ring can be described by the following two parameters: the phase angle of pseudorotation P and the amplitude of pseudorotation θm. These parameters are calculated from the endocyclic torsion angles νi by the equations given in [32]. The tables also include the endocyclic torsion angle ν2 (C1′–C2′–C3′–C4′), which provides information on the degree of staggering of substituents at the C2′ and C3′ atoms of the pentofuranose ring. The mutual orientation of the pentofuranose ring and the heterocyclic base is determined by the glycosidic angle χ (C4pur (C2pyr)–N9pur (N1pyr)–C1′–O4′), according to the IUPAC–IUB rules [33]. Two regions of the angle χ are distinguished: syn (0° ± 90°) and anti (180° ± 90°). Additionally, highlight areas high syn (χ from 90° until ~120° in anti region) and high anti (χ from −90° until ~−60° in syn region). Scheme 2 shows these four regions of χ for uridine.
The phase angle of pseudorotation can be related to the description of the conformation of the furanose ring that is based on displacements of atoms from the mean plane of the ring. The pentofuranose ring is generally non-planar and it can adopt an envelope conformation (iE), in which four atoms lie in a single plane, or a twist conformation (iTj), in which two adjacent atoms are displaced in the opposite directions from the mean plane of the ring. The indices correspond to the numbers of the atoms that are displaced from the mean plane of the ring, with the index 0 referring to the O4’ atom. The indices of the atoms that are displaced towards the C4’–C5’ bond are given as superscripts. The twist conformations are denoted by placing the index of the atom most displaced from the mean plane of the ring before the letter T, while the adjacent atom less displaced from the mean plane follows T.
The angle of pseudorotation of each unsymmetrical twist conformation varies over a range of 18° (Figure 5). Therefore, the character of atomic displacements in each of 20 sectors of the pseudorotation cycle is preserved, i.e., the numbers of the atoms displaced from the mean plane of the ring, the directions of these displacements, and the ratio of the displacements (in magnitude) remain unchanged. Either symmetrical twist conformations or envelope-type conformations bound the sectors of the pseudorotation cycle.

1.4. Conformations of Free Nucleosides

The conformations of nucleosides in the free form were studied in detail. The frequency of the occurrence of the parameters P and θm in crystals of 178 nucleosides was analyzed in [34]. The phase angles P of nucleosides were found in two ranges, from −1° to 34° and from 137° to 194°. The former range belongs to the N region (P varies from −45° to 45°), and the latter one to the S region (P is in the range of 135–225°). Only five nucleoside derivatives were found in the range from 84° to 106°, belonging to the E region (P is in the range of 45–135°). No conformers were found in the W region (P varies from 225° to −45°). The amplitude of pseudorotation θm of nucleosides in the crystal structures have a unimodal distribution over the range from 30° to 46°. It is almost independent of the type of the base and the phase angle P. The more planar the furanose ring, the smaller the parameter θm.
The results of which were analyzed in [35], the N and S conformers of nucleosides exist in a dynamic equilibrium, according to extensive NMR experiments in solution. The percentage ratio of the N and S conformers existing in equilibrium in solution depends on the nature of the nucleic acid base (purine or pyrimidine) and the type of the furanose ring (ribose or deoxyribose). Purine nucleosides prefer the S conformer, while pyrimidine nucleosides prefer the N conformation. The N↔S conformational interconversions occur through the E region of the pseudorotation cycle (at about P = 90°), because the energy barrier in this region is lower. The E barrier for the N↔S interconversion occurs due to steric strain that was caused by the eclipsed orientation of substituents at the C2′ and C3′ atoms at P = 90°. For the alternative pathway through the W region in the vicinity of the phase angle P = 270°, the energy barrier is much higher. In this region, in addition to the steric strain between the substituents at the C2′ and C3′ atoms, there is also steric strain between the nitrogenous base and the exocyclic 5′-oxymethyl group due to their close arrangement of these bulky substituents.
Different computational methods evaluated the energies of barriers to pseudorotation of derivatives of the furanose ring in the W and E regions. The analysis of the results of these calculations provided the estimate of their average values [36]. The barrier of pseudorotation in the W region is ~24 kJ/mol for 2′-deoxyribofuranose and ~31 kJ/mol for ribofuranose, according to these estimations. The E region is characterized by the barrier of ~7.5 kJ/mol for 2′-deoxyribofuranose and ~16 kJ/mol for ribofuranose. The barrier of pseudorotation of purine nucleosides in the E region, which was experimentally evaluated from the 13C NMR data, is 20 ± 2 kJ/mol [37]. It was suggested that the energy barrier for pyrimidine nucleosides is higher than 25 kJ/mol.
In the solutions, purine nucleosides occur with equal frequency in syn- and anti-conformations, whereas pyrimidine nucleosides more often exist in the anti-conformation [35,36]. The rotation about the glycosidic bond is not free [38]. In the case of pyrimidine nucleosides, the interconversions between these conformations are associated with steric strain between the protons at the C2’ and C6 atoms in the χ angle range near the high-anti region (from −60° to −90°) and steric interaction between the proton at the C2’ atom and O2 atom in the χ angle range in the vicinity of the high-syn region (90–120°). The energy of the barrier to syn ↔ anti transitions near the high-syn region was experimentally evaluated at 43–46 kJ/mol from the analysis of NMR spectroscopic data for pyrimidine nucleosides [39].

2. Discussion

2.1. Conformations of Nucleosides and Their Analogues in the Active Sites of PNPs and MTAP with Hexameric Quaternary Structure

Table 1 presents the conformational parameters of nucleosides in 47 complexes, including complexes with hexameric PNPs from B. cereus, Vibrio cholera, Helicobacter pylori, E. coli, Toxoplasma gondii, and B. subtillis, and complexes with hexameric MTAP from Sulfolobus solfataricus. In 37 complexes, the active sites contain a sulfate (phosphate) ion, and in 10 complexes this ion is absent. It should be noted that there are unacceptably short interatomic contacts and non-standard bond angles of nucleosides in the structure of Helicobacter pylori PNP in complex with formycin A (PDB 6F4X). Hence, we re-refined this structure to calculate the conformational parameters of the nucleoside.
Table 1 shows that the phase angles P of all the nucleosides vary over a wide part of the left part of the pseudorotation cycle (206–318°). The following two ranges of P values can be distinguished: (206–254°) and (258–318°). In the structures of the complexes containing a sulfate or phosphate ion, the nucleosides adopt a conformation that corresponds to the former range. The exceptions are two complexes of MTA with MTAP that are characterized by slightly larger angles P (259° and 267°). The conformations in the former region belong to one of the following four types: 3T4, 4T3, 4TO, and OT4. Only the 3T4 conformation is observed in the crystals of free nucleosides [34]. In the complexes, in which a sulfate (phosphate) ion is absent in the active site, namely, six complexes in the structure 1VHW, three complexes in the structure 6F4X (monomers 1, 2, and 4), and one of the complexes in 6F4X, in which phosphate is present with one-half occupancy (monomer 6), the angle P is in the range of 258–318°. These are the OT4, OT1, 1TO, and 1T2 conformers. These conformers were not found for nucleosides in the free form. In three of them (OT4, OT1, and 1TO), the endocyclic oxygen atom is displaced from the mean plane of the ring in the direction opposite to the glycosidic bond and the C4′–C5′ bond. This displacement of the O4′ atom (that is most pronounced in the OT4 and OT1 conformers) results in these bonds having axial positions. As mentioned above, the potential energy of these nucleoside conformations is high due to steric strain at P = 270° (W region).
In 43 complexes, purine bases adopt a high-syn (χ is in the range of 93–120°) orientation or they are in the immediate vicinity to this conformational region (χ is in the range of 127–131°). In all of these structures, two pairs of atoms of the nucleosides (N3 and C2’; C8 and O4’) form rather short contacts (about 3 Å). In the other four complexes (these are four of the seven complexes with formycin A in the structure 6F4X; Table 1, complexes 1, 2, 4, and 6), nucleosides adopt a high-anti orientation (χ varies in the range from −67° to −71°). In these conformers, there are short contacts between the N8 and C2’ atoms (the interatomic distance is about 3 Å). No correlation between the angle χ and presence of a sulfate or phosphate ion in the complex was revealed.
Pseudorotation angles of purine nucleosides and their analogs in the active sites of hexameric PNPs are shown in Figure 5 in red in the presence of sulfate (phosphate) ions, and in green when sulfate (phosphate) ions are absent.
The puckering amplitudes θm of all conformers in the active sites of hexameric PNPs and MTAP (except for the structure 4D9H) are in the range of 17–35°. In the structure 4D9H, the nucleoside adopts a conformation with a nearly planar furanose ring (θm = 4°), which is not typical of nucleosides. This might be attributed to the fact that the nucleoside with one-half occupancy is only present in one of the two crystallographically independent molecules. It is worth noting that not all atoms of the nucleoside are well fitted to the 2F0–Fc electron density at the 1σ level. Hence, the conclusions of the study [47] should be considered with caution.

2.2. Conformations of Nucleosides in the Active Sites of PNPs and MTAP with Trimeric Quaternary Structure

Table 2 presents the conformational parameters of nucleosides in complexes with trimeric Mycobacterium tuberculosis, Schistosoma mansoni, and calf spleen PNPs, and in the complex with trimeric MTAP from human placenta. There are a total of nine complexes, eight of which contain a sulfate (phosphate) ion.
The phase angles P of all the nucleosides in the complexes containing a sulfate (phosphate) ion are in the range of 214–243°, which is narrower than the range of P for nucleosides in complexes with hexameric PNPs, as it is seen from the Table; the angle χ is in the range of 126–133° and corresponds to the anti region in the immediate vicinity of the high-syn region. These structures also have rather short interatomic contacts similar to those found in hexameric PNPs. The puckering amplitudes θm are about 25°.
In one of the three complexes of inosine with Shistosoma mansony PNP (PDB 3FAZ), a sulftate ion is absent. In this complex, inosine adopts another conformation: χ is in the high-syn region (χ = 113°), the furanose ring is much more planar, and the phase angle belongs to the right part of the pseudorotation cycle (P = 136°).
Pseudorotation angles of purine nucleosides and their analogs in the active sites of trimeric PNPs in Figure 5 are shown in violet in the presence of sulfate (phosphate) ions and, when sulfate (phosphate) ions are absent, they are shown in blue.

2.3. Conformations of Nucleosides in the Active Sites of UPs

Table 3 presents the conformational parameters of nucleosides in 14 complexes with UPs from Vibrio cholera, Shewanella oneidensis, Schistosoma mansoni, and Salmonella typhimurium. Six of these 14 complexes do not contain sulfate ions.
The data in Table 3 show that, in all six complexes of thymidine with Vibrio cholerae UP (PDB 4LZW) [52], sulfate ions are absent, thymidine has an anti orientation (χ is in the range of −180°), and the furanose rings adopt 3T4 or 3T2 conformations (P is 191–203°).
In the dimeric structure 4TXJ [26], all four crystallographically independent complexes of Shistosoma mansoni UP with thymidine contain the nucleoside in the immediate vicinity of the high-syn region (χ is in the range of 122–130°), and the furanose rings adopt the OT4 and OT1 conformations (P is in the range of 266–275°). Sulfate ions are present in all active sites.
Uridine adopts a nearly identical conformation in the active site of Shewanella oneidensis UP (PDB 4R2W) [31] in the presence of sulfate (χ = 133°, P = 266°, OT4). Therefore, thymidine and uridine adopt similar conformations in the presence of sulfate, i.e., the presence or the absence of the 2′-OH group in the nucleoside is not a crucial factor that is responsible for the conformation.
In Figure 5, the pseudoratation angles of uridine or thymidine in the active sites of UP in the presence of sulfate ions are shown as yellow and, when sulfate ions are absent, they are shown as light pink.
The conformation of 2,2’-O-anhydrouridine is high-syn (χ = 111°) with P ~230°. This conformation is observed in all three active sites in complexes with Salmonella typhimurium UP (PDB 3FWP) [53] and it is determined by the C2’–O–C2 covalent bond. The rigid structure of 2,2’-O-anhydrouridine is well accommodated by the active site of uridine phosphorylase. The constant Ki of this substrate analogue to UP is only eight times lower as compared to Km of Urd and 13 times lower than Km of dUrd [54].

3. Conclusions

The conformations of nucleosides and their analogues in complexes with nucleoside phosphorylases determined at 2 Å resolution or higher were analyzed. In all complexes with nucleoside phosphorylases, nucleosides adopt similar conformations. They are significantly different from those of free nucleosides. The pentofuranose rings of all nucleosides in complexes are located within the W region of the pseudorotation cycle. Some of them are near the energy barrier of the N↔S interconversion. The presence of sulfate (or phosphate) ions in the structures limits the range over which the conformations of the pentofuranose rings can be varied. These limitations are associated with the fact that sulfate (or phosphate) ions form additional hydrogen bonds with atoms of nucleosides in the complexes. Most of the nucleosides (both purine and pyrimidine) are in a high syn orientation (in the immediate vicinity of the barrier to syn ↔ anti transitions at about χ = 120°). The syn orientation is energetically highly unfavorable for pyrimidine nucleosides in the free form.
To sum up, nucleosides adopt sterically strained conformations in the complexes. An increase in the potential energy of nucleosides in complexes is comparable with the energy of barriers to the N↔S interconversion through the W region and the height of the barrier to the syn ↔ anti transition in free nucleosides. The binding of nucleosides in strained conformations is a characteristic feature of the enzyme–substrate complex formation for this group of enzymes and it plays an important role in the mechanism of enzymatic catalysis.

Funding

This work was supported by the Russian Science Foundation (project No. 16-14-00178).

Acknowledgments

We are grateful to Zarubin Yu.P. for providing us with the program for calculating the parameters for pseudorotation within the pentofuranose cycle.

Conflicts of Interest

The authors declare no conflict of interest.

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Biomolecules 10 00552 sch001 550
Scheme 1. Phosphorolysis of nucleosides catalyzed by nucleoside phosphorylases (NPs). Urd—uridine, UP—uridine phosphorylase, PNP—purine nucleoside phosphorylase, Rib-1-P—α-d-ribose-1-phosphate, Pi—inorganic phosphate ion.
Scheme 1. Phosphorolysis of nucleosides catalyzed by nucleoside phosphorylases (NPs). Urd—uridine, UP—uridine phosphorylase, PNP—purine nucleoside phosphorylase, Rib-1-P—α-d-ribose-1-phosphate, Pi—inorganic phosphate ion.
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Figure 1. Superposition of the three-dimensional structures of hexameric PNP from B. cereus (PDB 3UAW, gold), trimeric PNP from Bos Taurus (PDB 1B80, lilac), and hexameric UP from Shewanella oneidensis (PDB 4R2W, green). The structures are superimposed with the COOT program.
Figure 1. Superposition of the three-dimensional structures of hexameric PNP from B. cereus (PDB 3UAW, gold), trimeric PNP from Bos Taurus (PDB 1B80, lilac), and hexameric UP from Shewanella oneidensis (PDB 4R2W, green). The structures are superimposed with the COOT program.
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Figure 2. Structure of the active site of hexameric B. cereus PNP in complex with adenosine and sulfate (PDB 3UAW). The nitrogen, oxygen, and sulfur atoms are shown in blue, red, and yellow, respectively. The carbon atoms of the protein and the substrate are gold and violet, respectively. The atoms of the adjacent subunit of the PNP dimer are colored in grey. The hydrogen bonds involving the adenosine molecule and sulfate are indicated by dashed lines.
Figure 2. Structure of the active site of hexameric B. cereus PNP in complex with adenosine and sulfate (PDB 3UAW). The nitrogen, oxygen, and sulfur atoms are shown in blue, red, and yellow, respectively. The carbon atoms of the protein and the substrate are gold and violet, respectively. The atoms of the adjacent subunit of the PNP dimer are colored in grey. The hydrogen bonds involving the adenosine molecule and sulfate are indicated by dashed lines.
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Figure 3. Structure of the active site of Shewanella oneidensis MR-2 UP in complex with uridine and sulfate (PDB 4R2W). The nitrogen, oxygen, and sulfur atoms are shown in blue, red, and yellow, respectively. The carbon atoms of the protein and the substrate are green and violet, respectively. The atoms of the adjacent subunit of the UP dimer are colored in grey. The hydrogen bonds involving the uridine molecule and sulfate are indicated by dashed lines.
Figure 3. Structure of the active site of Shewanella oneidensis MR-2 UP in complex with uridine and sulfate (PDB 4R2W). The nitrogen, oxygen, and sulfur atoms are shown in blue, red, and yellow, respectively. The carbon atoms of the protein and the substrate are green and violet, respectively. The atoms of the adjacent subunit of the UP dimer are colored in grey. The hydrogen bonds involving the uridine molecule and sulfate are indicated by dashed lines.
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Figure 4. Structure of the active site of trimeric Bos Taurus PNP (PDB 1B80). The nitrogen, oxygen, and sulfur atoms are shown in blue, red, and yellow, respectively. The carbon atoms of the protein and the substrate are lilac and violet, respectively. The hydrogen bonds involving the immucillin H molecule and phosphate are indicated by dashed lines.
Figure 4. Structure of the active site of trimeric Bos Taurus PNP (PDB 1B80). The nitrogen, oxygen, and sulfur atoms are shown in blue, red, and yellow, respectively. The carbon atoms of the protein and the substrate are lilac and violet, respectively. The hydrogen bonds involving the immucillin H molecule and phosphate are indicated by dashed lines.
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Scheme 2. The mutual orientation of the pentofuranose ring and the heterocyclic base for uridine are shown: anti and syn (above, side view) and high anti and high syn (below, view from above).
Scheme 2. The mutual orientation of the pentofuranose ring and the heterocyclic base for uridine are shown: anti and syn (above, side view) and high anti and high syn (below, view from above).
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Figure 5. Pseudorotation cycle of the pentofuranose ring in nucleosides. Sectors are named as iTj and iTj for unsymmetrical twist conformations (the character of atomic displacements from the mean plane of the ring is preserved in each sector). Pseudorotation angles of purine nucleosides and their analogs in the active sites of hexameric PNPs in the presence of sulfate (phosphate) ions are shown in red, while when sulfate (phosphate) ions are absent they are shown in green. Pseudorotation angles of purine nucleosides and their analogs in the active sites of trimeric PNPs in the presence of sulfate (phosphate) ions are shown in violet, while when sulfate (phosphate) ions are absent they are shown in blue. Pseudoratation angles of uridine or thymidine in the active sites of UP in the presence of sulfate ions are shown as yellow, while when sulfate ions are absent they are shown as light pink.
Figure 5. Pseudorotation cycle of the pentofuranose ring in nucleosides. Sectors are named as iTj and iTj for unsymmetrical twist conformations (the character of atomic displacements from the mean plane of the ring is preserved in each sector). Pseudorotation angles of purine nucleosides and their analogs in the active sites of hexameric PNPs in the presence of sulfate (phosphate) ions are shown in red, while when sulfate (phosphate) ions are absent they are shown in green. Pseudorotation angles of purine nucleosides and their analogs in the active sites of trimeric PNPs in the presence of sulfate (phosphate) ions are shown in violet, while when sulfate (phosphate) ions are absent they are shown in blue. Pseudoratation angles of uridine or thymidine in the active sites of UP in the presence of sulfate ions are shown as yellow, while when sulfate ions are absent they are shown as light pink.
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Table
Table 1. Conformations of nucleosides and their analogues in the active sites of hexameric PNPs and 5′-deoxy-5′-methylthioadenosine phosphorylases (MTAP).
Table 1. Conformations of nucleosides and their analogues in the active sites of hexameric PNPs and 5′-deoxy-5′-methylthioadenosine phosphorylases (MTAP).
OrganismEnzymeComplexPDB IDRes. ǺRef.ShapeP deg.θm deg.ν2 deg.χ deg.
B. cereusAdoP; w.t.Ado, SO43UAW1.20[29]4T322930−20+103
B. cereusAdoP; w.t.Ino, SO43UAX1.20[29]4T322630−21+101
B. cereusAdoP; D204N mutantAdo, SO43UAY1.40[29]4T322928−18+102
B. cereusAdoP; D204N mutantIno, SO43UAZ1.40[29]4T322329−21+99
V. choleraPNP; w.t.Ado1VHW1.54[40]OT127527+2+94
OT127728+3+93
OT126729−1+95
OT127626+3+93
OT1269270+95
OT127330+2+97
H. pyloriPNP; w.t.formycin A PO4 (in monomers 3, 5 and 6)6F4X1.694[41]1TO/OT4305/25817/299.9/−6.2−67/+118
1T230833+20−69
OT425433−9+127
1T231830+22−71
4T323032−20+120
OT127828+4−67
B. cereusAdoP; w.t.Ado, SO42AC71.7[42]4T322630−21+105
4T322729−20+106
E. coliPNP; w.t.formycin A, PO44TS91.77[43]3T421224−20+107
3T421325−21+108
4T322028−21+116
S. solfataricusMTAP; w.t.Guo, SO41JE11.8[24]4T322423−16+107
4T322225−19+103
4T322123−17+107
4T322528−20+109
4T322627−18+106
4T322928−18+108
E. coliPNP D204A, R217A double mutantformycin A, PO4, SO44TTJ1.87[43]4TO23628−16+106
4T322330−22+115
4TO24127−13+105
E. coliPNP; w.t.formycin A, PO43UT61.89[44]3T420628−25+104
4T322026−20+106
4T322130−22+114
E. coliPNP; w.t.2-fluoro Ado, PO41PK91.9[45]4TO24134−17+116
4TO23635−19+106
4TO23632−18+106
T. gondiiPNP; w.t.immucillin H, PO43MB81.9[46]4TO23432−19+130
4T323234−21+130
B. subtilisBsPNP-233; w.t.Ado4D9H1.91[47]3T244+4+107
S. solfataricusMTAP; w.t.MTA, SO41JDT2.0[24]OT426732−2+131
OT425430−9+127
OT425928−5+120
S. solfataricusMTAP; w.t.Ado, SO41JDV2.0[24]4TO23728−15+115
4TO23827−14 +119
4TO23828−15 +120
4TO23728−15 +114
Note: there are two variant structures of complexes of the first monomer of structure 6F4X, which are separated by “/”.
Table
Table 2. Conformations of nucleosides and their analogues in the active sites of trimeric PNPs and MTAP.
Table 2. Conformations of nucleosides and their analogues in the active sites of trimeric PNPs and MTAP.
OrganismEnzymeComplexPDB IDRes. ǺRef.ShapeP deg.θm deg.ν2 deg.χ deg.
Bos taurusPNP; w.t.Immucillin H, PO41B801.5[30]4TO23929−15+133
H sapiensMTAP; w.t.MTA, SO41CG61.7[48]4TO24132−15+126
M. tuberculesisPNP; w.t.Immucillin H, PO41G2O1.75[49]3T421520−16+129
4T321718−15+130
3T421421−17+128
S. mansonyPNP; w.t.Ino, and SO4 in monomers 1 and 33FAZ1.9[50]4TO23828−15+131
1T213612−8+113
4TO23728−16+131
Calf spleanPNP; w.t.Ino, SO41A9S2.0[51]4TO24327−12+133
Table
Table 3. The conformations of nucleosides in the active sites of UPs.
Table 3. The conformations of nucleosides in the active sites of UPs.
OrganismEnzymeComplexPDB IDRes. ǺRef.ShapeP deg.θm deg.ν2 deg.χ deg.
V. choleraeUP, w.t.Thd4LZW1.29[52]3T420330−27+167
3T219133−32+178
3T219530−30−178
3T420330−28+165
3T219333−33+177
3T219530−29−180
S. oneidensisUP, w.t.Urd, SO44R2W1.60[31]OT426620−2+133
S. mansoniUP, w.t.Thd, SO44TXJ1.66[26]OT426433−4+125
OT426631−2+122
OT127531+3+124
OT4269310+130
S. typhimuriumUP, w.t.2,2’-O-anhydrouridine, SO43FWP1.86[53]4T323029−19+111
4TO23432−19+111
4T323130−19+110

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Il’icheva, I.A.; Polyakov, K.M.; Mikhailov, S.N. Strained Conformations of Nucleosides in Active Sites of Nucleoside Phosphorylases. Biomolecules 2020, 10, 552. https://0-doi-org.brum.beds.ac.uk/10.3390/biom10040552

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Il’icheva IA, Polyakov KM, Mikhailov SN. Strained Conformations of Nucleosides in Active Sites of Nucleoside Phosphorylases. Biomolecules. 2020; 10(4):552. https://0-doi-org.brum.beds.ac.uk/10.3390/biom10040552

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Il’icheva, Irina A., Konstantin M. Polyakov, and Sergey N. Mikhailov. 2020. "Strained Conformations of Nucleosides in Active Sites of Nucleoside Phosphorylases" Biomolecules 10, no. 4: 552. https://0-doi-org.brum.beds.ac.uk/10.3390/biom10040552

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