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Article

The Impact of Viral Infection on the Chemistries of the Earth’s Most Abundant Photosynthesizes: Metabolically Talented Aquatic Cyanobacteria

by
Yunpeng Wang
1,2,*,
Scarlet Ferrinho
1,2,
Helen Connaris
1,2 and
Rebecca J. M. Goss
1,2,*
1
School of Chemistry, University of St Andrews, North Haugh, St Andrews KY16 9AJ, UK
2
Biomedical Sciences Research Complex, University of St Andrews, North Haugh, St Andrews KY16 9SX, UK
*
Authors to whom correspondence should be addressed.
Biomolecules 2023, 13(8), 1218; https://0-doi-org.brum.beds.ac.uk/10.3390/biom13081218
Submission received: 31 May 2023 / Revised: 17 July 2023 / Accepted: 24 July 2023 / Published: 4 August 2023
(This article belongs to the Special Issue Marine-Derived Molecules with Different Bioactivities)
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Abstract

:
Cyanobacteria are the most abundant photosynthesizers on earth, and as such, they play a central role in marine metabolite generation, ocean nutrient cycling, and the control of planetary oxygen generation. Cyanobacteriophage infection exerts control on all of these critical processes of the planet, with the phage-ported homologs of genes linked to photosynthesis, catabolism, and secondary metabolism (marine metabolite generation). Here, we analyze the 153 fully sequenced cyanophages from the National Center for Biotechnology Information (NCBI) database and the 45 auxiliary metabolic genes (AMGs) that they deliver into their hosts. Most of these AMGs are homologs of those found within cyanobacteria and play a key role in cyanobacterial metabolism-encoding proteins involved in photosynthesis, central carbon metabolism, phosphate metabolism, methylation, and cellular regulation. A greater understanding of cyanobacteriophage infection will pave the way to a better understanding of carbon fixation and nutrient cycling, as well as provide new tools for synthetic biology and alternative approaches for the use of cyanobacteria in biotechnology and sustainable manufacturing.

1. Introduction

More than half of the oxygen in the atmosphere is generated by oxygenic photosynthetic bacteria, known as cyanobacteria [1]. These bacteria, whose biomass exceeds the total mass of zooplankton and fish, play a central role in carbon capture, the release of dissolved organic carbon (DOC), and nutrient cycling (Scheme 1A). It is becoming increasingly apparent that cyanobacterial metabolic pathways, and nutrient cycling itself, are largely governed by the bacteriophages that infect them. With approximately 10 million viruses in every milliliter of seawater [2] and representing more than 94% of nucleic acid entities in the oceans [3], oceanic viruses are ~10-fold more abundant than marine bacteria and considerably outnumber other marine organisms, such as phytoplankton, zooplankton, and organisms at higher trophic levels [1,4].
Based on published data regarding labile DOC release mediated by microbial and zooplankton activities, the partitioning of inputs among these sources suggests (even though much remains to be understood about the exact nature of the chemistry of these short-lived species) that about 20% of labile DOC is released through excretion and death processes of heterotrophic organisms, approximately 40% originates directly from phytoplankton (including cyanobacteria) through extracellular release during photosynthesis, and around 40% is released through phytoplankton death processes, including senescence, sloppy feeding, and viral and fungal lysis [5,6]. The viral shunt, through the incorporation of carbon in new viruses and the release of carbon during cell lysis, represents a considerable pathway through which DOC is mobilized and redistributed within ecosystems. It is estimated that as much as one-quarter of DOC undergoes the viral shunt process (Scheme 1B) [7]. This highlights the important role that viruses play in the dynamics of carbon cycling, shaping carbon availability and utilization by other organisms in the ecosystem. A greater understanding of the viral shunt process and the chemistries encoded by viruses will provide insights into the intricate connections between viruses, host cells, and the carbon cycle, with implications for ecosystem functioning and biogeochemical processes [7].
Biomolecules 13 01218 sch001
Scheme 1. A simplified summary of the central metabolic role that aquatic viruses play. (A) Cyanophages play a key role in the mortality and life cycle of photosynthetic bacteria, while other aquatic viruses impact unicellular eukaryotic grazers. These organisms, at the bottom of the food chain, in turn impact all organisms in the ocean and are thus central to carbon flux. (B) The steady-state model of organic carbon. (Adapted from reference [7]). All values are expressed in terms of the flux of photosynthetically fixed carbon (100%) The assumption is made that all organic carbon, present in pelagic waters, is readily accessible to bacteria and is eventually respired, with minimal loss through export.
Scheme 1. A simplified summary of the central metabolic role that aquatic viruses play. (A) Cyanophages play a key role in the mortality and life cycle of photosynthetic bacteria, while other aquatic viruses impact unicellular eukaryotic grazers. These organisms, at the bottom of the food chain, in turn impact all organisms in the ocean and are thus central to carbon flux. (B) The steady-state model of organic carbon. (Adapted from reference [7]). All values are expressed in terms of the flux of photosynthetically fixed carbon (100%) The assumption is made that all organic carbon, present in pelagic waters, is readily accessible to bacteria and is eventually respired, with minimal loss through export.
Biomolecules 13 01218 sch001
Infection of a cyanobacterium by a virus known as a “cyanophage” or “cyanobacteriophage” can be persistent or lethal, ultimately resulting in host lysis and the release of the progeny phage. In both types of infection, host and phage may exchange genetic material. This results in cyanophage often encoding metabolism-related genes, analogous to cyanobacterial genes. These genes, known as auxiliary metabolic genes (AMGs), play a key role in host metabolism following infection. Here, we analyze AMGs from 153 publicly accessible cyanophage genomes [8,9,10,11] (see Table 1) with the aim of gaining greater insight into the ways in which cyanophages manipulate host metabolism. Cyanophages exhibit a broad distribution across diverse aquatic environments worldwide (Table 1). They are prevalent in both freshwater and marine ecosystems, encompassing lakes, rivers, ponds, oceans, and estuaries. The geographic range of cyanophages can vary, influenced by factors such as local environmental conditions, host cyanobacteria species, and the presence of suitable habitats conducive to their replication and survival.

2. Overview of Cyanophage

Cyanophages play a crucial role in shaping both the local and global ecosystems. Here we extracted the genomes of each fully sequenced cyanophage, deposited in the NCBI database (Table 1). For these 153 cyanophage, in Table 1, we record their taxonomy, host, number of proteins, genes and tRNA and geographic distribution. We explored the annotation of each cyanophage genome and noted 45 regularly occurring AMGs, which may play a key role in altering host metabolism following infection (Table 2). To verify the homology with known genes, we analyzed each AMG in each cyanophage genome using BLAST. Gene functions were deduced through the corresponding literature.
Cyanobacteriophages can be categorized, according to their morphology, into three families: Myoviridae and Podoviridae with a GC content of 40% and 46%, respectively, and Siphoviridae with the highest GC content (56%). (Figure 1A). Cyanophages belonging to Myoviridae, (myocyanophages), have markedly larger genomes (x = 184.18 kb, standard deviation (SD) = 23.04 kb) than those of Podoviridae and Siphoviridae (x = 44.95 kb, SD = 10.83 kb, x = 42.58 kb, SD = 15.36 kb, respectively), and are speculated to use a different strategy to take over the host.
Recent studies show that the encoded tRNA genes within the myocyanophage can alter the host codon translational efficiency [35,36]. This provides a competitive advantage, enabling the myocyanophage to infect a much broader range of hosts than their podocyanophage and siphocyanophage counterparts [36,37,38]. The divergence of nucleotide sequences from different cyanophages may be seen through comparative genome analysis (Figure 1B). DNA polymerase and terminase are widely distributed in the podocyanophages and myocyanophages. DNA polymerase has not been discovered in most of the siphocyanophages, whereas terminase exists in most of the siphocyanophages. We based the phylogenetic analysis on the nucleotide sequences of the conserved genes, DNA polymerase and terminase, using the maximum likelihood method (ML) (Figure 1B). Generally, close genetic relationship may be seen within families, and a more distinct genetic relationship may be seen between families, though notable exceptions may be observed.
The 153 fully sequenced cyanophages from NCBI comprise 33 podocyanophages, 103 myocyanophages, 15 siphocyanophages, and 2 unclassified phages. We identified and discounted all genes associated with phage replication from these sequences. In the remaining sequence data, a palette of 45 AMGs that may play a key role in altering host metabolism following infection could be seen to occur frequently. Though a few AMGs were seen to occur in only one virus, many were seen to be widely distributed across the majority of viruses that were analyzed (Figure 2), implying the importance and evolutionary advantage of the roles that they play. The 45 AMGs encoded within the cyanophages were all homologs of genes found within cyanobacteria that play a key role in cyanobacterial metabolism. In addition to genes encoding the assembly of new cyanophage genes relating to enhancing photosynthesis that dominate, genes relating to central carbon metabolism, phosphate metabolism, methylation, and regulation are prevalent. Genes with sequence similarity to known halogenases are also abundant. This global analysis and distribution of the AMGs are presented in Figure 2. The AMGs and their potential role as the cyanophage infects the host cyanobacterium are reviewed in the following sections.
After infection, cyanophage AMGs are expressed, impacting photosynthesis, carbon metabolism, phosphate metabolism, and regulation. The resulting energy and flux of precursors are redirected toward phage DNA synthesis and capsid production. The counterparts of numerous AMGs linked to photosynthesis and central carbon metabolism may be seen in the genomes of myocyanophages. This is undoubtedly their origin. As shown above (Figure 1A), myocyanophages generally have considerably larger genomes than the other cyanophages and possess a long contractile tail enabling the delivery of this much larger genome into bacteria. This tail is sheathed and operates via a contractile mechanism, resulting in the highly efficient injection of its DNA into the host. As a result of housing a much larger genome, the heads of myocyanophages are larger than those of other cyanophages. This larger casing, more sophisticated injection machinery, in combination with the much larger genome, places a greater metabolic burden on the host. Correspondingly, almost all of the myocyanophages can be seen to carry multiple AMGs that potentially force the upregulation of photosynthesis to sequester further carbon and the upregulation of carbon metabolism to generate the substantial additional energy needed. Curiously, two exceptions emerge: Synechococcus phage-S-H34, (Figure 2, entry 88), an outlier in terms of its much higher GC content, appears not to have any AMGs related to photosynthesis. Synechococcus phage-BUCT-ZZ01 (Figure 2, entry 84), one of the smaller myocyanophage, appears to not have any AMGs related to glycolysis. The availability of phosphate is essential to the generation of new DNA as the phage replicates. So far, the myocyanophage alone may be seen to possess AMGs linked to the modulation of phosphate metabolism. It is puzzling that one of the larger myocyanophages, Synechococcus phage-ACG-2014f, does not have any AMGs related to phosphate metabolism (Figure 2, entry 96).
In analyzing the data, we sought first to explore genes linked to photosynthesis, with the question in mind as to what extent might viral infection enhance photosynthesis and thus carbon capture and oxygen production?
Of the 153 viral DNA sequences analyzed, almost all could be seen to contain AMGs encoding proteins with a role in photosynthesis, with the potential to augment or control the infected host’s photosynthetic activity. Only seven of the candidates did not clearly show the presence of such a gene. AMGs related directly to the enhancement of photosynthesis, including genes encoding the production of the photosystem II reaction center protein, the production of the high-light inducible protein, the antennae protein as well as the production of photosynthetic pigments, were clearly visible in many of the viruses.

3. The Photosynthetic Machinery Used by Cyanobacteria

Cyanobacteria have evolved to adapt quickly and are able to efficiently capture light across a wide range of different wavelengths using a range of photosynthetic pigments including carotenoids, chlorophylls, and the tetrapyrrolic derivatives of chlorophylls. Cyanobacteria utilize photosynthetic machinery comprising three multi-subunit complexes, photosystems I and II (PSI, PSII) [39] plus the additional phycobilisomes (PBS) that attach to the cytoplasmic surface of thylakoid membranes and closely interact with PSII [40] (Figure 3A). The PBS comprise phycolipoproteins (PBPs) together with linker proteins. The PBPs are water soluble and brightly colored. The color arises from the attachment of a series of tetrapyrrole chromophores via a thioester linkage to a cysteine residue within the PBP [41]. The PBPs are subdivided into three groups relating to the energy they absorb and to the tetrapyrrole chromophore. The allophycocyanins (APCs) are chromophoric proteins that absorb low-energy light and make up much of the core of the PBS. At the base of the rods, next to the core, are the phycocyanins (PCs), which absorb intermediate-energy light, and at the far end of the rod are the phycoerythrins (PEs), which absorb high-energy light [42].
Marine and freshwater cyanobacteria, red algae, and cryptophytes all produce and use a subclass of the light-harvesting pigment phycoerythrin (PE) that is pink. This open-chain tetrapyrrole pigment is called phycoerythrobilin (PEB) 1. In addition to PE, marine cyanobacteria also use the pigment phycourobilin (PUB), which is an isomer of PEB. The PE produced by freshwater cyanobacteria contains solely PEB, in contrast to their marine counterparts, which carry both PUB and PEB. Using PEs, within the PBS, cyanobacteria can absorb light between 450 and 700 nm at depths deeper than 200 m in the ocean where low light levels render chlorophyll ineffective.

3.1. AMGs with the Potential to Impact the Biogenesis of the PBS

Photosynthetic pigments are biosynthesized and then formed into the PBS, an assemblage of chromophorylated proteins anchored to thylakoid membranes. In cyanobacteria, the biosynthesis of PEB 1, as well as the associated pigments phycocyanabilin 2 and phytochromobilin 3, starts with the heme oxygenase (HO)-mediated cleavage of heme to an open-chain product biliverdin IXa (BV) 4 through an aerobic reaction [47,48]. Of the 153 sequenced cyanophage genomes, 8 could be seen to contain a gene encoding an additional copy of HO (XIII in Figure 2 and Figure 3B. Table 2).
Ferredoxin oxidoreductase, encoded by pebA, is employed to catalyze the two-electron reduction of biliverdin IXa (BV) 4 to 15,16 dihydrobiliverdin (DHBV) 5 [49]. The conversion of DHBV 5 to phycoerythrobilin (PEB) 1 is subsequently mediated by PebB (Phycoerythrobilin: ferredoxin oxidoreductase). The direct conversion of BV 4 to PEB 1 mediated by PebS (phycoerythrobilin synthase), is also possible. Intriguingly, of the 153 sequenced cyanophage analyzed, 9 of them may be seen to contain a gene encoding PebS (VII in Figure 2 and Figure 3B; Table 2).
Phycocyanobilin:ferredoxin oxidoreductase (PcyA) belongs to the ferredoxin-dependent bilin reductase (FDBR) family [50,51] and mediates the conversion of BV 4 to phycocyanabilin (PCB) 2. Through the catalysis of PcyA, the substrate BV 4 can be converted to phycocyanobilin (PCB) directly [50,52]. The gene encoding PcyA may be seen in 16 of the 153 sequenced genomes (IV in Figure 2 and Figure 3B, Table 2). Once the chromophores are biosynthesized, they are integrated into the PBPs through the formation of thioester links with cysteine residues. They are then assembled into the PBS. A putative phycobiliprotein lyase (CpeT) [8], likely involved in supporting the attachment of PEB to the apo-phycobiliprotein, provides a scaffold for the pigment to ensure binding in the right conformation to facilitate correct stereospecific ligation to a conserved cysteine residue within the phycobiliprotein. CpeT belongs to the so-called T-type lyases, and phycocyanobilin-specific homologs of CpeT (i.e., CpcT) were shown to specifically serve the position equivalent to cysteine 153 in the -subunit of phycocyanin (PC) [53]. Therefore, the analogous CpeT lyases may serve the Cys-153 of PE. It has been speculated that their expression also contributes to phage fitness by enhancing the light-harvesting capacity. In this regard, the cyanophage S-PM2 was shown to induce increased synthesis of the light-harvesting PE in Synechococcus sp. WH7803 during infection [54]. CpeT was found in 31 of the 153 sequenced genomes (VI in Figure 2 and Figure 3B, Table 2)
Notably, it is only the myocyanophages that are seen to carry the AMGs HO, PcyA, and PebS responsible for the biosynthesis of photosynthetic pigments. Similarly, it is only the myocyanophages that carry the gene CpeT, responsible for the ligation of the photosynthetic pigments to the light-harvesting protein complex. The only exception seen in the set of viruses analyzed is that PebS is also encoded by the single podovirus cyanophage 9515-10a. Perhaps it is that among the three families only the myocyanophages have adapted to benefit from enhancing their host cell’s biosynthetic capability, or the other two families of much smaller phages (podocyanophages and siphocyanophages) do not require increased carbon flux.

3.2. AMGs with the Potential to Repair Photosystem II following Oxidative Damage

As a large common protein complex, photosystem II (PSII) extracts the electrons from water and releases molecular oxygen [55]. Polypeptide D1 and D2, encoded by psbA and psbD, respectively, form the core heterodimer of the PSII reaction center. Together with the necessary cofactors, PSII enables the generation of a strong oxidant that can be used to extract electrons from water (Figure 3C). The generation of this strong oxidant results in abundant levels of molecular oxygen, which can cause photo-oxidative damage. The polypeptides D1 and D2, especially D1, are particularly vulnerable to photodamage [56]. This could irreversibly inhibit the function of PSII. Stress conditions, such as high light, high salt concentration, or high or low temperatures, may exacerbate the damage [57]. To counteract the damage, cyanobacteria have evolved a PSII repair mechanism, whereby damaged D1 can be degraded and complemented by the newly synthesized D1 polypeptides. If the synthesis of D1 polypeptides can complement the damage, then photosynthesis would not be affected. However, if the synthesis of D1 polypeptides cannot complement the damage, photoinhibition occurs, and the growth of cells would be affected. In a recent study, the phototrophs treated with protein synthesis inhibitors were shown to inhibit the synthesis of D1 polypeptides and result in photoinhibition [58,59]. This demonstrates that D1 peptide synthesis is especially important for the PSII repair cycle against photoinhibition. Different species and growth conditions can affect the half-life of D1 polypeptides. For example, in higher plants, the half-life of D1 is about 2–6 h. The half-life of D1 in Synechococcus WH7803 is about 3–4 h [57].
Photodamage to the D1 polypeptide is unavoidable, regardless of the prevailing light conditions. Most of the investigated viruses contain AMGs encoding D1 (111/153). Based on our analyses, 16/33 podocyanophages and 95/103 myocyanophages contain a copy of psbA. AMGs encoding D2 were also prevalent in the myocyanophages analyzed (78/103) but absent in podoviridae and siphoviridae.
Previous studies have indicated that shortly after infection, archetypal myovirus T4 can block host DNA replication, transcription, and translation and degrade the host DNA [57]. In Synechococcus WH7803 cells, S-PM2 infection exerts a similar effect on the host. One effect of infection would be to prevent the D1 protein re-synthesis. Indeed, the transcription of the gene psbA is markedly and progressively reduced following S-PM2 infection [57]. Thus, compared with uninfected cells, the infected cells would be more sensitive to photoinhibition. This also explains why the acquisition of a copy of the psbA gene would confer a fitness advantage to the phage. By encoding its own copy of D1, S-PM2 maintains the PSII repair cycle after the synthesis of D1 proteins in host cells have been shut down, thus permitting the continuation of photosynthesis and providing the necessary energy for phage replication.
It could be postulated that after infection, more psbA genes need to be transcribed to complement the loss of D1 polypeptides. The psbD AMG-encoding D2 polypeptide was found in 78 of the 153 cyanophage that we analyzed.
In Prochlorococcus myoviruses P-SSM2 and P-SSM4 [60], there are putative promoter and transcriptional terminators flanking the genes psbA and psbD, indicating they can be expressed autonomously [61]. Gene psbA and the essential and highly expressed phage capsid genes in the podocyanophage P-SSP7 have the same promoters and terminators, enabling simultaneous transcription, implying that psbA has become an integral part of phage genomes [61].

3.3. AMGs with the Potential to Impact Electron Transport

Plastocyanin (PC), encoded by the gene petE, can transfer the electron flow from cytochrome b6f to photosystem I in cyanobacteria (XII in Figure 2 and Figure 3D). However, studies indicate that PC encoded by cyanophages may have a different function [50]. The hydrophobic core region of PC directly follows the signal peptide of the cyanophage PC without a C-terminal domain [62]. The N-terminal signal peptide of the cyanophage PC is also modified. Compared with the host, cyanophage PC also contains many unique amino acid residues [38]. In cyanobacteria, the isoelectric point (pI) is essential for the interactions of PC and PSI. However, the cyanophage PC has a substantially different pI compared to the host (Krukal–Wallis: H1 = 50.21, p < 0.001). All of these findings may imply a potential functional difference in PC between the host and cyanophage. After the acceptance of electrons from the cytochrome b6f complex, cyanophage PC may not reduce the PSI, but could, instead, transfer electrons to cytochrome c oxidase (COX) directly [63]. Following the reduction of COX, the proton is transferred across the thylakoid [59]. Through this electron transfer pathway, electrons could accumulate for ATP synthesis by ATP synthase. Furthermore, the over-production of the PQ pool (plastoquinol, reduced; plastoquinone, oxidized) caused by the cyanophage infection may be alleviated in this way, which could prevent the photosystem from undergoing photoinhibition [38]. Of the 153 sequenced cyanophage genomes, 70 of them contain the PC-encoded gene petE and are all from myocyanophages. According to a recent study, following phage infection, the COX expression level may be enhanced, which may support the above hypothesis for the role of this complex in accepting electrons from the plastocyanin (PC) pool during infection [46].
Cyanophages can also encode type I NAD(P)H dehydrogenase, also known as the NDH -1 complex, which can transfer electrons to quinol and participate in photosynthesis and CO2 uptake [64]. Through metagenomic analysis, cyanophages have been shown to have the ndhI gene-encoded NADH dehydrogenase subunit 1 [65], the ndhD-gene encoded NADH dehydrogenase subunit 4 [66], as well as the ndhP gene-encoded single transmembrane small subunit of the NDH-1 complex [67]. In the genome of P-TIM40, we also identified the ndhI gene (IX in Figure 2 and Table 2) that encoded a Fe-S protein. The cysteine residues that directly bind the two Fe–S centers (N6a and N6b) of the protein are completely conserved in the suspected cyanophage homolog, suggesting that NdhI may play a key role in electron transfer through the complex directly to reduce plastoquinol [65]. Electrons are then transferred from plastoquinol to PC through the cytochrome b6f complex. To date, the mechanism of electron donation to NdhI has not been clearly understood, although there are several hypotheses [64,68,69]. It is also difficult to know whether there are any functional differences between cyanobacterial NdhI and the cyanophage NdhI.
In cyanobacteria, ferredoxin (Fd), encoded by the petF gene, can transfer electrons from photosystem I to ferredoxin NADP+ reductase (FNR), which, in turn, reduces NADP to NADPH for carbon fixation (Figure 3D). However, according to some hypotheses, electron flow may be redirected from Fd to the NDH-1 complex after infection [38]. Furthermore, the ferredoxin-dependent bilin reductases (FDBRs) can catalyze BV reduction, where Fd provides electrons for this reaction [70]. As a class of radical enzymes, FDBRs are able to catalyze the biosynthesis of both PEB and PCB [50,70,71]. Among the 153 sequenced cyanophage genomes, the gene petF encoding Fd was found in 44 of them. In a recent study, the transcriptome difference between a cyanophage (P-HM2) and its host (Prochlorococcus MED4) throughout infection was analyzed. Following infection, the host transcription levels of photosystem I assembly protein Ycf37, FNR, and other translation-related-genes were diminished, suggesting that these could affect the reducing power flow during the Calvin cycle carbon fixation [46].
A putative plastoquinol terminal oxidase (PTOX) is commonly found in cyanophage genomes [21]. Of the 153 sequenced cyanophages, PTOX is observed in 35 of these genomes, notably only pertaining to a subset of cyanophages known as myocyanophages. PTOX is thought to be a safety valve in these phages to protect photo electron transfer (PET) proteins from photodamage, especially under stressful conditions [38]. When the PQ pool is over reduced, often, as a result of high light or low iron conditions, electrons can be donated to oxygen through PTOX [72,73]. This is a particularly important flow of electrons when considering the viral-induced inhibition of PSI and FNR [46]. This effect could explain why PTOX expression levels are seen to be upregulated in cyanophage-infected cells and exist in many cyanophages.
High-light-inducible proteins (HLIPs) can also be found in many cyanophage genomes (90/153). In cyanophages, the function of HLIPs has been an enigma until recently, as very little information has been available on the role of HLIPs in the infection process. However, a recent knockout study of hli genes in Synechocystis sp. PCC6803 showed that knockout mutants were sensitive to high irradiances [74,75]. HLIPs, which contain one membrane-spanning helix and are located in the thylakoid [74], could associate with both PSII [76] and PSI [77]. The study demonstrated that HLIPs were constructive to PSI stabilization [77], tetrapyrrole biosynthesis regulation [78], the transient store of chlorophyll during PSII repair and assembly [79], and excess excitation energy dissipation [38,75]. It is likely that cyanophage HLIPs have similar functions to cyanobacterial HLIPs.
There are many genes involved in photosynthesis that are encoded within cyanophage genomes (Table 1). In addition to this, the expression levels of some host-encoded photosynthesis genes (such as COX) increased after infection. Transcription levels of ferredoxin NADP+ reductase (FNR) and some photosystem I proteins were diminished, leading to the reduction of NADPH biosynthesis. More electrons were transferred to COX through the cyanophage PC, meaning more protons could accumulate for ATP synthesis, resulting in more energy for cyanophage DNA biosynthesis.

4. AMGs with the Potential to Impact Central Carbon Metabolism

In cyanobacteria, energy (ATP) and reducing power (NADPH) produced by ATP synthase and ferredoxin NADP+ reductase (FNR) during photosynthesis are mostly used for carbon fixation in the Calvin cycle [80]. However, cyanophage infection can drastically alter the energy flow. This research reveals that more than 20% of cyanophage genomes contain a cp12 gene, which encodes for a Calvin cycle inhibitor. In cyanobacteria, the Calvin cycle is a key pathway for carbon fixation. Two essential enzymes of the Calvin cycle are phosphoribulokinase (PRK) and glyceraldehyde-3-phosphate dehydrogenase (GADPH/Gap2). The first step of the cycle, PRK, catalyzes the conversion of D-ribulose 5-phosphate (Ru5P) to D-ribulose 1,5-biphosphate (RuBP), consuming ATP [81]. GADPH catalyzes the conversion of D-glycerate 1,3-biphosphate (BPG) to the end product glyceraldehyde 3-phosphate (GAP), consuming NADPH (Scheme 2A). The Calvin cycle inhibitor CP12 can inhibit the function of these two enzymes by redirecting the carbon flux from GAP synthesis to the pentose phosphate pathway (PPP) and can reduce the consumption of ATP and NADPH. In a study of CP12, particularly in the light and dark cycle of Prochlorococcus, researchers found that the cp12 gene was maximally expressed at night, and in that in dark cycle, carbon flux was directed from the Calvin cycle to PPP in Prochlorococcus [82].
In addition to the Calvin cycle, PPP also plays a key role in central carbon metabolism in cyanobacteria [63]. Three PPP enzymes were found in the sequenced cyanophage genomes, i.e., transaldolase (talc, 96/153), glucose-6-phosphate dehydrogenase (zwf, 25/153), and 6-phosphogluconate dehydrogenase (gnd, 22/153) (Scheme 2A). The sequence of TalC is substantially different from that of its host homolog TalB. Through multiple sequence alignment analysis, when compared with TalB, TalC contained numerous deletions; however, there were no changes in the active site residues that were important for catalysis [83,84]. Through activity analysis, the product of the genes talC and talB could catalyze the conversion of sedoheptulose 7-phosphate (S7P) and GAP to erythrose 4-phosphate (E4P) and fructose 6-phosphate (F6P) (Scheme 2A) [63]. Based on a comparison of the kinetic properties of TalCs with TalBs, the phage transaldolase may have more advantages than their host homologs to enhance PPP in the host during the infection [63].
The PPP gene zwf, encoding glucose-6-phosphate dehydrogenase (G6PDH), catalyzes the conversion of glucose-6-phosphate to ribulose 5-phosphate and produces NADPH and CO2. The 6-phosphogluconate dehydrogenase, encoded by gnd, catalyzes the conversion of gluconate 6-phosphate to gluconolactone 6-phosphate and produces NADPH. Currently, there are only two known enzymes shown to produce NADPH in the PPP. However, until now, little research has been done to explore the cyanophage zwf and gnd genes. This study shows that zwf and gnd are present in 25/153 and 22/153, respectively, of the sequenced cyanophage genomes.
The expression of the phage talC, gnd, and zwf genes may augment the PPP and produce more NADPH and the R5P that phages need for dNTP biosynthesis [63]. It is likely that after infection more NADPH would be directed toward phage DNA synthesis through the expression of these four phage genes. In the same study, researchers also found that after infection, the host NADPH/NADP ratio increased twofold, which was consistent with the inhibition of the Calvin cycle and the enhancement of PPP [63]. In a recent study [85], about 4 h infection with phage S-PM2 resulted in the cessation of CO2 fixation in Synechococcus sp. WH7803. For phage S-RSM4, after about 2 h infection, the CO2 fixation in WH7803 was stopped. This is consistent with our analysis. In addition, with the phage genes cp12 gnd, zwf, and talc, S-RSM4 can suspend the CO2 fixation of the host faster than phage S-PM2 without these genes.
Through metagenomics analysis, the glycogen biosynthetic gene encoded by the glgA gene was identified from the viromes isolated from the Pacific Ocean [86]. The glgA gene-encoded enzyme catalyzes the conversion of G6P to glycogen, suggesting that virus infection may induce a starvation response in the host and direct more carbon flux to the glycolysis pathway [87]. The mannose-6-phosphate isomerase, encoded by manA and involved in mannose metabolism, was also identified [86]. Mannose-6-phosphate isomerase is known to convert M6P to F6P for use in glycolysis [88] (Scheme 2A).
Global warming has considerable implications for aquatic ecosystems, triggering various changes. With rising water temperature, dissolved oxygen levels can drop, and changes in both pH and salinity can occur [89]. These changes directly impact cyanobacteria, with rising temperatures and CO2 levels likely to promote cyanobacterial blooms [90]. There is potential for these blooms to, once again, bring balance, restoring depleted oxygen levels, and to an extent, provide a screen, shading the underlying water from direct sunlight. However, these blooms are limited by nutrient availability. Increasing cyanobacterial levels are likely to result in increased populations of phages. Lysis caused by phage infection results in the re-release of limiting nutrients, enabling the cycle to continue and possibly enabling the further restoration of restored oxygen.
In a recent study, the transcription levels of SLC13 permease, sulfate adenylyl-transferase, adenylyl-sulfate kinase, phosphoadenosine phosphosulfate reductase, assimilatory sulfite reductase, and cysteine synthase A O-acetylserine sulfhydrylase were increased after infection of Pseudoalteromonas by cyanophage HP1. These genes are involved in the assimilatory sulfate reduction pathway and the L-cysteine biosynthesis pathway (Scheme 2B) [91,92]. Researchers posited that the synthesis of cysteine in infected cells provided more energy because cysteine can be degraded to acetyl-CoA for energy production in the TCA cycle [93]. From the Pacific Ocean Virome dataset, components of the TCA cycle have been identified including aconitase (acn), isocitrate dehydrogenase (icd), 2-oxoglutarate dehydrogenase (sucABCD), isocitrate lyase, succinate dehydrogenase (sdh), and fumarate hydratase (fum). Malate synthase A (aceAB), involved in the glyoxylate shunt, was also identified [86]. In the host Pseudoalteromonas infected by cyanophage HP1, the most highly expressed genes (~3-fold) were aceA and aceB involved in the glyoxylate shunt [91]. The glyoxylate shunt, which is used to increase cellular ATP synthesis and reducing power, is commonly observed under various stressors [94,95].
In addition, two viral gene families were identified from the Pacific Ocean Virome dataset. The fatty acid oxidation complex (fadB), the long-chain fatty acid transporter (fadL) involved in the fatty acid metabolic pathway, acetyl-CoA carboxylase (acc), propionyl-CoA carboxylase (pcc), and methylmalonyl-CoA epimerase/mutase (mcm) are involved in the 3-hydroxypropionate (3HP) cycle. Considerably more energy can be generated by fatty acid oxidation, and TCA cycle intermediates can be balanced during the infection process. Carbon flux can also be redirected from fixation to energy production by the genes acc, pcc, mce, and mcm involved in the 3HP cycle. Through this reprogramming, the carbon and nitrogen cycles may also be influenced (Scheme 2B) [96].
All these changes in central carbon metabolism are consistent with the hypothesis that viruses can redirect carbon flux away from carbon fixation pathways towards dNTP and energy production, which can be used for the generation and assembly of cyanophages.

5. AMGs with the Potential to Impact Regulatory Factors

Among the 153 sequenced cyanophage genomes, 65 cyanophages possess putative sigma factors (RpoD/RpoS) (Figure 2). The cyanophage putative sigma factors have high amino acid sequence homology with σ70-type sigma factors in cyanobacteria [97]. As a transcription initiator, the sigma factor binds to the specific sequence and regulates gene transcription. The metabolism, growth, and phenotype of bacteria are affected by the expression of sigma factors. In Escherichia coli, there are several sigma factors, including σ70 (RpoD), which is responsible for exponential growth, σ38 (RpoS), which is a stationary phase sigma factor, and the flagellar sigma factor σ28 (RpsD) [98,99]. Cyanobacteria also possess sigma factors in the σ70 family, of which there are three groups categorized upon their amino acid sequences, including sigma factors that control the expression of essential cell growth genes [100], sigma factors that are not essential for cell survival, and other σ70 factors. Furthermore, there are several other putative sigma factors such as σ38 in cyanophage genome sequences. In a recent study, the carbon metabolism in Synechococcus elongatus PCC 7942 was modified through the expression of putative sigma factors from Synechococcus phages. After the expression of putative RpoD from phage S-CBS2 and putative RpsD4 from phage S-CBS3, the growth of S. elongatus PCC 7942 was enhanced noticeably. Through metabolome analysis, the acetyl-CoA concentration was found to increase 3.2-fold and 1.9-fold following expression of the putative RpoD from S-CBS2 and the putative RpsD4 from S-CBS3, respectively [97]. It is possible that the host carbon metabolism can be affected by sigma factors encoded by the cyanophages after infection.
Some genes involved in cofactor B12 synthesis have also been discovered in the sequenced cyanophage genomes including the genes cobA (38/153), cobO (37/153), and cobS (52/153). As an ancient enzyme, ribonucleotide reductase (RNR) reduces ribonucleotides to deoxyribonucleotides before DNA synthesis. Depending on their interaction mechanism with oxygen and the different ways for the generation of thiol radicals, RNRs can be grouped into three classes through their reactivity with O2 [101]. Class I RNRs are O2-dependent; class II RNRs are O2-independent; and class III RNRs are O2-sensitive. Adenosylcobalamin (cofactor B12) is a key cofactor of class II RNRs that are commonly observed in cyanophages and cyanobacteria. Genes cobA, cobO, and cobS, which were found in the sequenced cyanophage genomes, also play a key role in the adenosylcobalamin (cofactor B12) biosynthesis pathway (Scheme 2C) [102].
The gene mazG encoding dNTP pyrophosphatase activity, MazG, was found in 64 of the 153 sequenced cyanophage genomes (Figure 2). In E. coli, MazG acts on the signaling nucleotide guanosine tetraphosphate (ppGpp) with one-third of the E. coli genome being regulated by MazG [103]. As a regulator, MazG responds to nutrient stress and programmed cell death [104,105]. In cyanophages, as a global transcriptional regulator through the modulation of ppGpp levels, MazG regulates many metabolic pathways and may also extend the cell survival period under phage infection stress [106]. However, MazG has high non-canonical NTP specificity, indicating the need for the identification of the substrate for each MazG by solving crystal structures and activity analysis [107]. The exact function and substrate of cyanophage MazG would, therefore, need to be cautiously interpreted [21].
As a translational repressor, the RegA protein regulates the expression of several phage early mRNAs in the bacteriophage T4 [108]. In the sequenced cyanophage genomes, 48/153 RegA proteins were observed. In the process of bacteriophage T4 infection, DNA polymerase accessory proteins and membrane proteins of undefined functions are regulated by RegA. These proteins, affected by gene regA, may all function in T4 DNA metabolism [109]. Also, the RegA protein may inhibit translation by binding to target mRNAs and blocking the formation of the ribosome initiation complex [110].
The ribonucleoside diphosphate reductase reduces ribonucleosides to ribonucleotides, which is the rate-limiting step of DNA synthesis [111]. Nrd genes are found across a wide range of coliphage [112] and environmental [113] phage genomes, with 48 of the 153 sequenced cyanophage genomes containing nrd genes. In a recent study where the marine host Pseudoalteromonas bacterium was infected by two different viruses, siphovirus PSA-HS2 and podovirus PSA-HP1, the temporal fold value of gene nrdA changed from 4 to 205 and frm 2 to 239, respectively, despite the fact that the nrd genes were not found in these two phages [68]. This suggests that ribonucleoside diphosphate reductase plays a key role in phage DNA synthesis after infection.
Twenty of the sequences contained genes that potentially encode flavin-dependent halogenases (FDHs). This group of enzymes is known to introduce a halogen into molecules in the form of a carbon–halogen bond. Halogenated molecules exhibit a wide range of biological activities, bioavailability, and metabolic stability [114,115]. This bond has been shown to increase the thermal, oxidative stability, and permeability of a compound compared with the non-halogenated molecule [116]; thus, a host’s metabolite activity and stability could be enhanced by halogenation resulting from cyanophage infection.

6. AMGs with the Potential to Impact Phosphate Metabolism

As a scarce resource in the oceans, phosphorus is often thought to be a growth-limiting factor for cyanobacteria [117]. Phosphorus also plays a key role in phage replication. Therefore, it is not surprising that 101 phosphate-inducible phoH genes and 31 phosphate-uptake pstS genes were identified in the sequenced cyanophage genomes (Figure 2). The gene phoH is widely distributed in cyanobacteria, eubacteria, and archaea [118]. This gene is induced under phosphate stress in E. coli [119] although its function has not yet been experimentally verified. Bioinformatic analyses have shown that phoH genes are likely part of a multi-gene family with different functions, including phospholipid metabolism, RNA modification, as well as fatty acid beta-oxidation [118]. As an important gene in the phosphate-transport system, pstS is also distributed widely in cyanobacteria, eubacteria, and archaea [120]. Under phosphate stress conditions, the gene pstS can modulate phosphate absorption and assimilation and contribute to the pyrolysis cycle in the host cell [21,121]. Both the phoH and pstS genes might improve phosphorus acquisition during the infection of the host cells.

7. AMGs with the Potential to Impact Methylation

Methylation plays a key role in the infection process. In the sequenced cyanophage genomes, DNA methylase (23/153), DNA adenine methylase (55/153), cytosine methyltransferase (11/153), and type II N-methyl DNA methyltransferase (13/153) were found to be involved in methylation. DNA coding and base-pairing functions are not affected by methylation modifications. In prokaryotes, modified bases appear primarily to protect the host from the infection of bacteriophages (and other genomic parasites) [122].

8. Biosynthetic Gene Clusters (BGCs): What Is the Function of BGCs in Phage?

Bacteria can produce diverse metabolites, which are a rich source of industrially relevant natural products. The synthetic pathway of these metabolites is encoded by groups of genes called biosynthetic gene clusters (BGCs). BGCs can affect the interaction and competence among microorganisms. Further, BGCs are extremely important for the mining of new bioactivities [123]. In a recent study, the structure and function of phage-encoded BGCs were investigated [123]. pBGCs are rare, and most of them reside within temperate phages, which infect commensal or pathogenic bacterial hosts. The majority of pBGCs were found to encode for bacteriocins [123], which have been shown to provide a clear competitive fitness advantage for the infected bacterium. Through genetic and genomic comparisons, researchers revealed a strong association between the pBGC type and phage host range, which demonstrated bacteriocins are encoded in temperate phages of a few commensal bacterial genera, and lysogenic conversion also provides an evolutionary benefit to the phage itself [123].
In our review, we also investigated the presence of BGCs in the 153 publicly accessible cyanophage genomes using antiSMASH. However, no BGCs in the 153 cyanophage genomes were predicted—an unsurprising revelation due to the documented rarity of pBGCs. A previous study utilized 10,062 high-quality phage genomes, which were available in the PATRIC database, and detected the presence of pBGCs in 69 genomes. Furthermore, 15,184 high-quality bacterial genomes available from NCBI were screened by ProphET to extract prophage regions and were subsequently screened by antiSMASH for the detection of pBGCs. This revealed the presence of only 307 pBGC regions [123]. However, with the increased discovery and genomic sequencing of cyanophages, it is likely that more pBGCs will be discovered. Understanding the natural function and distribution of pBGCs may provide insight into the mechanisms through which cyanophages infect, control, and utilize the host.

9. Response of Hosts to Cyanophage Infection

In the previous part of the review, we focused on how cyanophages affected their hosts using the AMGs followed by a discussion of the response of hosts to the infection of cyanophages. After cyanophage infection, most host gene expression is suppressed. However, some host gene expression is enhanced.
In a recent study [124], researchers replaced general medium using medium containing 98 atoms% 15NO3− as the sole N source after the S-SM1 phage had infected Synechococcus WH8102. They found 12 host-encoded proteins that continued to be produced despite the general suppression of host gene expression. Of these 12 proteins, 8 of them have homologs (i.e., AMGs) in the genomes of S-SM1 or other phages and/or on putative viral genome fragments from aquatic viral metagenome datasets. These eight proteins included heme oxygenase, methionyl aminopeptidase, ribosomal protein S21, glycogen phosphorylase, phosphogluconate dehydrogenase (Gnd), transaldolase (TalC), cysteine synthase A, and the CP12 repressor. Methionyl aminopeptidase removes initiator methionine residues, which is the second step in the N-terminal methionine excision (NME) pathway of protein maturation. NME is necessary for the stable assembly of the D1/D2 reaction center of photosystem II [125], so the continued production of methionyl aminopeptidase may be linked to phage PSII protein expression. Ribosomal protein S21 is necessary for mRNA binding and translation initiation [126], which can enhance viral fitness. We have discussed the other six proteins in the previous review.
The synthesis of the other 4 of the 12 host proteins may point to cellular defense mechanisms against phage infection. The Rho termination factor is involved in the silencing of foreign DNA elements as a defense against the deleterious effects of their expression [127]. Ribonuclease J may act to degrade phage transcripts and inhibit infection progress [124].
This study suggests that phages without some AMGs may also maintain the function of some metabolism pathways, which are necessary for the phages using the hosts’ genes (such as S-SM1). This study may also explain why there are more AMGs in the myocyanophages than in the other two types of cyanophages. It is likely that the production of some of the host gene’s proteins is not enough for myocyanophages growth and may need more energy, thus having more AMGs present than the other two types of cyanophages.

10. Conclusions

In the aqueous system, cyanobacteria can be lysed by cyanophages, which, in turn, release dissolved carbon and nutrients. The dissolved organic matter (DOM) plays essential roles in the ocean’s recycling system. By creating sticky lysates, phages can effectively shuttle organic carbon from the surface to the deep ocean. Cyanophages play a vital role in the evolution of cyanobacteria, exerting control over cyanobacterial abundance, population dynamics, and the structure of natural communities. They serve as a global reservoir of genetic information, functioning as vectors for gene transfer and endowing cyanobacteria with new properties that influence the rate and direction of evolutionary processes. Lysogeny markedly contributes to the maintenance of the gene pool and facilitates ecological adaptation among cyanobacteria. The integration of multiple cyanobacterial genes into cyanophage genomes provides evidence of genetic transfer between hosts and phages, which drives adaptive microevolutionary processes. Molecular analyses of cyanophage–host interactions strongly support the concept of coevolution between cyanophages and cyanobacterial genomes [128].
From the 1990s until now, there has been an increase in the number of cyanophages that have been isolated and their genomes sequenced. It is evident that there are many homologous genes between cyanophages and their host, and it is possible for genetic material exchange and cell metabolism to occur after infection. In our review, all publicly accessible cyanophage genomes in NCBI were found, and 45 AMGs, which play a key role in the metabolism process, were identified in these cyanophage genomes. These were evaluated and categorized into five categories. It was shown that there are twelve genes involved in photosynthesis, six genes involved in carbon metabolism, five genes involved in methylation, three genes involved in phosphate metabolism, and 15 regulatory factor genes. After phage infection, the biosynthesis of PBS is enhanced through the expression of photosynthesis family genes from cyanophages. In order to supply more ATP for cyanophage DNA biosynthesis, the expression levels of genes involved in photosynthesis are also enhanced. Cyanophages also redirect carbon flux away from carbon fixation pathways towards dNTP and energy production, which may be used for the generation and assembly of cyanophages. Through the enhancement of phosphate metabolism genes, increased phosphate can be used for augmented DNA synthesis following infection. The regulatory genes in cyanophages can improve the activity and stability of some metabolism pathways. Finally, the methylation-related genes can improve the infection process by preventing host cleavage. Together, these AMGs not only play a beneficial role in the cyanophage during the infection cycle but, on the whole, appear to make a substantial contribution to increasing photosynthesis and global oxygen levels. The ability to enhance an infected host’s biosynthetic capability confers potential advantage to a phage, providing further carbon flux that may be channeled toward phage replication [54,55,129]. From an ecological and biotechnological viewpoint, an enhanced understanding of these infection processes holds the opportunity to use them as non-GM tools for organism engineering, enhancing their usefulness as chemical factories and potentially harnessing them for accelerated carbon capture and oxygen generation. The role of cyanophages in contributing to specialized metabolites, such as the production of halometabolites, is intriguing and will form the core of prominent future research.

Author Contributions

Data analysis, Y.W.; supported by R.J.M.G. and H.C.; Writing—All authors contributed to the writing, review and editing of the manuscript. All authors have read and agreed to the published version of the manuscript.

Funding

We thank BBSRC BB/T017058/1 (Y.W.) IBioIC, MASTS, Xanthella (S.F.), and the Royal Society (R.J.M.G.) for financial support.

Data Availability Statement

All data analysed in this study is obtained from the NCBI database. https://0-www-ncbi-nlm-nih-gov.brum.beds.ac.uk/, accessed on 30 May 2023.

Conflicts of Interest

The authors declare no conflict of interest.

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Biomolecules 13 01218 sch002
Scheme 2. AMGs involved in central carbon metabolism. (A) Cyanophage genes involved in the Calvin–Benson cycle and pentose phosphate pathway. After infection, the expression of cyanophage gene cp12 can inhibit the Calvin cycle and carbon fixation. The expression of cyanophage genes talC, zwf, and gnd can enhance the PPP. Pi, phosphate; CbbA, fructose-1,6-bis P/sedoheptulose-1,7-bis-P aldolase; GlpX, fructose-1,6/sedoheptulose-1,7-bisphosphatase; Pgi, P-glucose isomerase; Pgk, P-glycerate kinase; Pgl, 6-P-gluconolactonase; PrkB, P-ribulokinase; RbcLS, ribulose-1,5-bis-P carboxylase/oxygenase; Rpe, ribulose-5-P epimerase; RpiA, ribulose-5-P isomerase; TktA, transketolase; Tpi, triose-P isomerase; BPG, 2,3-bis-P-glycerate; DHAP, DHA P; E4P, erythrose 4-P; FBP, fructose 1,6-bisP; F6P, fructose 6-P; GAP, glyceraldehyde 3-P; G6P, glucose 6-P; PGA, 3-P-glyceric acid; R5P, ribose 5-P; RuBP, ribulose 1,5-bis-P; Ru5P, ribulose 5-P; SBP, sedoheptulose 1,7-bis-P; 6PG, 6-P-gluconate; 6PGL, 6-P-gluconolactone; S7P, sedoheptulose 7-P; and X5P, xylulose 5-P. (B) Cyanophage genes involved in L-cysteine biosynthesis (Purple), Tricarboxylic acid (TCA) cycle (Red), glyoxylate shunt (Blue), and 3-hydroxypropionate (Green). The genes (~3-fold) of the TCA cycle were those involved in the glyoxylate shunt. The glyoxylate shunt was used to increase the cellular reducing power and ATP for phage synthesis. (C) Cyanophage genes involved in the adenosylcobalamin (cofactor B12) biosynthesis pathway.
Scheme 2. AMGs involved in central carbon metabolism. (A) Cyanophage genes involved in the Calvin–Benson cycle and pentose phosphate pathway. After infection, the expression of cyanophage gene cp12 can inhibit the Calvin cycle and carbon fixation. The expression of cyanophage genes talC, zwf, and gnd can enhance the PPP. Pi, phosphate; CbbA, fructose-1,6-bis P/sedoheptulose-1,7-bis-P aldolase; GlpX, fructose-1,6/sedoheptulose-1,7-bisphosphatase; Pgi, P-glucose isomerase; Pgk, P-glycerate kinase; Pgl, 6-P-gluconolactonase; PrkB, P-ribulokinase; RbcLS, ribulose-1,5-bis-P carboxylase/oxygenase; Rpe, ribulose-5-P epimerase; RpiA, ribulose-5-P isomerase; TktA, transketolase; Tpi, triose-P isomerase; BPG, 2,3-bis-P-glycerate; DHAP, DHA P; E4P, erythrose 4-P; FBP, fructose 1,6-bisP; F6P, fructose 6-P; GAP, glyceraldehyde 3-P; G6P, glucose 6-P; PGA, 3-P-glyceric acid; R5P, ribose 5-P; RuBP, ribulose 1,5-bis-P; Ru5P, ribulose 5-P; SBP, sedoheptulose 1,7-bis-P; 6PG, 6-P-gluconate; 6PGL, 6-P-gluconolactone; S7P, sedoheptulose 7-P; and X5P, xylulose 5-P. (B) Cyanophage genes involved in L-cysteine biosynthesis (Purple), Tricarboxylic acid (TCA) cycle (Red), glyoxylate shunt (Blue), and 3-hydroxypropionate (Green). The genes (~3-fold) of the TCA cycle were those involved in the glyoxylate shunt. The glyoxylate shunt was used to increase the cellular reducing power and ATP for phage synthesis. (C) Cyanophage genes involved in the adenosylcobalamin (cofactor B12) biosynthesis pathway.
Biomolecules 13 01218 sch002
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Figure 1. Genomic and phylogenetic features of cyanophage families. (A) Scatterplot of the GC (guanine–cytosine) content and genome size. (B) Phylogenetic ML tree with related cyanophages based on the nucleotide sequences of DNA polymerase and terminase. The trees were constructed in MEGA version 7 by the ML method.
Figure 1. Genomic and phylogenetic features of cyanophage families. (A) Scatterplot of the GC (guanine–cytosine) content and genome size. (B) Phylogenetic ML tree with related cyanophages based on the nucleotide sequences of DNA polymerase and terminase. The trees were constructed in MEGA version 7 by the ML method.
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Figure 2. Widespread distribution of AMGs related to photosynthesis and carbon metabolism across the 153 cyanophage AMGs labelled I–XLV and cyanophages labelled 1–151. AMGs involved in photosynthesis and carbon metabolism. I. Photosystem II reaction center protein PsbA/D1, II. Photosystem II D2 protein, III. 2OG-Fe (II) oxygenase family oxidoreductase, IV. Phycocyanobilin: ferredoxin oxidoreductase (PcyA), V. Ferredoxin (PetF), VI. Phycoerythrobilin lyase (CpeT), VII. Phycoerythrobilin synthase (PebS), VIII. Phycoerythrobilin, IX. NADH dehydrogenase subunit I, X. NAD synthetase, XI. Plastoquinol terminal oxidase PtoX. XII. Plastocyanin (PetE), XIII. Heme oxygenase (HO), XIV. Hli gene family/high-light inducible protein, XV. Thioredoxin, XVI. RfaE bifunctional protein, XVII. Transaldolase family protein (TalC) in this family, XVIII. Glucose 6-phosphate dehydrogenase Zwf, XIX. 6-phosphogluconate dehydrogenase Gnd, XX. FAD-dependent thymidylate synthase/thymidylate synthase, XXI. Carboxylesterase, XXII. UDP-glucose 6-dehydrogenase, XXIII. Acyl carrier protein ACP, XXIV. Fructose-6-phosphate aldolase, XXV. Phage host specificity protein, XXVI. DNA methylase, XXVII. DNA adenine methylase Dam, XXVIII. Cytosine methyltransferase, XXIX. Type II N-methyl DNA methyltransferase (group alpha), XXX. Phosphate-starvation inducible protein (PhoH), XXXI. Phosphate-induced stress protein, XXXII. Phosphate ABC transporter phosphate-binding protein PstS XXXIII. Nrd, XXXIV. RNA polymerase sigma factor RpoD/rpoS late transcription sigma factor, XXXV. ERF family protein, XXXVI. MazG, XXXVII. RegA photosynthetic apparatus regulatory protein, XXXVIII. Transcriptional regulator, XXXIX. late promoter transcriptional accessory protein, XL. CP12 carbon metabolic regulator, XLI. MarR family protein XLII. Translational regulator, XLIII. XRE family transcriptional regulator, XLIV. CobS/CobO/CobA, XLV. Tryptophan halogenase-like gene. Cyanophage 1. Anabaena phage A-4L, 2. Cyanophage 9515-10a, 3. Cyanophage BHS3, 4. Cyanophage KBS-P-1A, 5. Cyanophage KBS-S-1A, 6. Cyanophage NATL1A-7, 7. Cyanophage NATL2A-133, 8. Cyanophage PP, 9. Cyanophage P-SSP2, 10. Cyanophage SS120-1, 11. Phormidium virus WMP3, 12. Phormidium virus WMP4, 13. Prochlorococcus phage P-GSP1, 14. Prochlorococcus phage P-RSP2, 15. Prochlorococcus phage P-SSP3, 16. Prochlorococcus phage P-SSP6, 17. Prochlorococcus virus PSSP7, 18. Synechococcus phage S-CBP4, 19. Synechococcus phage S-CBP42, 20. Synechococcus phage S-RIP1, 21. Cyanophage Pf-WMP3, 22. Cyanophage Pf-WMP4, 23. Synechococcus phage P60, 24. Synechococcus phage MRHenn-2013a, 25. Synechococcus phage S-SRP02, 26. Synechococcus phage S-SRP01, 27. Synechococcus phage S-SBP1, 28. Synechococcus phage S-B28, 29. Synechococcus phage S-CBP1, 30. Synechococcus phage S-CBP2, 31. Synechococcus phage S-CBP3, 32. Synechococcus phage S-RIP2, 33. Synechococcus virus Syn5, 34. Cyanophage P-TIM40, 35. Cyanophage S-RIM4, 36. Cyanophage S-RIM12, 37. Cyanophage P-RSM3, 38. Cyanophage S-RIM14, 39. Cyanophage S-RIM32, 40. Cyanophage S-RIM50, 41. Cyanophage S-SSM2, 42. Cyanophage S-SSM6a, 43. Cyanophage S-SSM6b, 44. Cyanophage S-TIM4, 45. Cyanophage S-TIM5, 46. Cyanophage Syn2, 47. Cyanophage Syn10, 48. Cyanophage Syn30, 49. Microcystis virus Ma-LMM01, 50. Prochlorococcus phage MED4-213, 51. Prochlorococcus phage P-HM1, 52. Prochlorococcus phage P-HM2, 53. Prochlorococcus phage P-RSM4, 54. Prochlorococcus phage P-SSM2, 55. Prochlorococcus phage P-SSM3, 56. Prochlorococcus phage P-SSM4, 57. Prochlorococcus phage P-SSM5, 58. Prochlorococcus phage P-SSM7, 59. Prochlorococcus phage Syn1, 60. Prochlorococcus phage Syn33, 61. Synechococcus phage KBS-M-1A, 62. Synechococcus phage S-B68, 63. Synechococcus phage S-CAM1, 64. Synechococcus phage S-CAM8, 65. Synechococcus phage S-CBM2, 66. Synechococcus phage S-IOM18, 67. Synechococcus phage S-PM2, 68. Synechococcus phage S-RIM2, 69. Synechococcus phage S-RIM8, 70. Synechococcus phage S-RSM4, 71. Synechococcus phage S-ShM2, 72. Synechococcus phage S-SKS1, 73. Synechococcus phage S-SM1, 74. Synechococcus phage S-SM2, 75. Synechococcus phage S-SSM4, 76. Synechococcus phage S-SSM5, 77. Synechococcus phage S-SSM7, 78. Synechococcus phage S-WAM1, 79. Cyanophage P-RSM1, 80. Cyanophage P-RSM6, 81. Cyanophage P-SS1, 82. Synechococcus phage S-WAM2, 83. Synechococcus phage S-CRM01, 84. Synechococcus phage BUCT-ZZ01, 85. Synechococcus phage S-H38, 86. Synechococcus phage S-SRM01, 87. Synechococcus phage S-N03, 88. Synechococcus phage S-H34, 89. Synechococcus phage B3, 90. Synechococcus phage B23, 91. Synechococcus phage S-SCSM1, 92. Synechococcus phage S-H68, 93. Synechoccus phage S-B43, 94. Synechococcus phage S-B05, 95. Synechococcus phage S-SZBM1, 96. Synechococcus phage ACG-2014f, 97. Synechococcus phage S-H9-1, 98. Synechococcus phage S-H9-2, 99. Synechococcus phage SynMITS9220M01, 100. Synechococcus phage S-H25, 101. Synechococcus phage S-P4, 102. Synechoccus phage S-E7, 103. Synechococcus phage S-B64, 104. Synechococcus phage Bellamy, 105. Synechococcus phage S-H35, 106. Synechococcus phage S-CAM9, 107. Synechococcus phage S-CAM7, 108. Synechococcus phage S-CAM4, 109. Synechococcus phage S-CAM3, 110. Synechococcus phage S-CAM22, 111. Synechococcus cyanophage S-WAM2, 112. Synechococcus cyanophage S-WAM1, 113. Synechococcus phage ACG-2014j, 114. Synechococcus phage ACG-2014i, 115. Synechococcus phage metaG-MbCM1, 116. Synechococcus phage ACG-2014g, 117. Synechococcus phage ACG-2014e, 118. Synechococcus phage ACG-2014d, 119. Synechococcus phage ACG-2014b, 120. Synechococcus phage ACG-2014a, 121. Synechococcus phage S-MbCM100, 122. Synechococcus phage S-MbCM25, 123. Synechococcus phage S-MbCM7, 124. Synechococcus phage S-RIM8 A.HR5, 125. Synechococcus phage S-RIM8 A.HR3, 126. Synechococcus phage S-RIM8 A.HR1, 127. Synechococcus phage S-RIM2 R9_2006, 128. Synechococcus phage S-RIM2 R1_1999, 129. Synechococcus phage S-RIM2 R21_2007, 130. Synechococcus phage Syn30, 131. Synechococcus phage ACG-2014c, 132. Synechococcus phage S-T4, 133. Synechococcus phage S-CBWM1, 134. Synechococcus phage ACG-2014h, 135. Synechococcus phage syn9, 136. Synechococcus phage Syn19, 137. Cyanophage KBS-S-2A, 138. Cyanophage MED4-117, 139. Cyanophage PSS2, 140. Microcystis phage MACPNOA1, 141. Prochlorococcus phage MED4-184, 142. Cyanophage S-2L, 143. Synechococcus phage S-H1, 144. Synechococcus phage S-LBS1, 145. Synechococcus phage S-CBS1, 146. Synechococcus phage S-CBS4, 147. Synechococcus phage S-CBS3, 148. Synechococcus phage S-CBS2, 149. Synechococcus virus S-ESS1, 150. Microcystis cyanophage vB_MaeS-yong1, 151. Microcystis cyanophage Mwe-Yong1112-1.
Figure 2. Widespread distribution of AMGs related to photosynthesis and carbon metabolism across the 153 cyanophage AMGs labelled I–XLV and cyanophages labelled 1–151. AMGs involved in photosynthesis and carbon metabolism. I. Photosystem II reaction center protein PsbA/D1, II. Photosystem II D2 protein, III. 2OG-Fe (II) oxygenase family oxidoreductase, IV. Phycocyanobilin: ferredoxin oxidoreductase (PcyA), V. Ferredoxin (PetF), VI. Phycoerythrobilin lyase (CpeT), VII. Phycoerythrobilin synthase (PebS), VIII. Phycoerythrobilin, IX. NADH dehydrogenase subunit I, X. NAD synthetase, XI. Plastoquinol terminal oxidase PtoX. XII. Plastocyanin (PetE), XIII. Heme oxygenase (HO), XIV. Hli gene family/high-light inducible protein, XV. Thioredoxin, XVI. RfaE bifunctional protein, XVII. Transaldolase family protein (TalC) in this family, XVIII. Glucose 6-phosphate dehydrogenase Zwf, XIX. 6-phosphogluconate dehydrogenase Gnd, XX. FAD-dependent thymidylate synthase/thymidylate synthase, XXI. Carboxylesterase, XXII. UDP-glucose 6-dehydrogenase, XXIII. Acyl carrier protein ACP, XXIV. Fructose-6-phosphate aldolase, XXV. Phage host specificity protein, XXVI. DNA methylase, XXVII. DNA adenine methylase Dam, XXVIII. Cytosine methyltransferase, XXIX. Type II N-methyl DNA methyltransferase (group alpha), XXX. Phosphate-starvation inducible protein (PhoH), XXXI. Phosphate-induced stress protein, XXXII. Phosphate ABC transporter phosphate-binding protein PstS XXXIII. Nrd, XXXIV. RNA polymerase sigma factor RpoD/rpoS late transcription sigma factor, XXXV. ERF family protein, XXXVI. MazG, XXXVII. RegA photosynthetic apparatus regulatory protein, XXXVIII. Transcriptional regulator, XXXIX. late promoter transcriptional accessory protein, XL. CP12 carbon metabolic regulator, XLI. MarR family protein XLII. Translational regulator, XLIII. XRE family transcriptional regulator, XLIV. CobS/CobO/CobA, XLV. Tryptophan halogenase-like gene. Cyanophage 1. Anabaena phage A-4L, 2. Cyanophage 9515-10a, 3. Cyanophage BHS3, 4. Cyanophage KBS-P-1A, 5. Cyanophage KBS-S-1A, 6. Cyanophage NATL1A-7, 7. Cyanophage NATL2A-133, 8. Cyanophage PP, 9. Cyanophage P-SSP2, 10. Cyanophage SS120-1, 11. Phormidium virus WMP3, 12. Phormidium virus WMP4, 13. Prochlorococcus phage P-GSP1, 14. Prochlorococcus phage P-RSP2, 15. Prochlorococcus phage P-SSP3, 16. Prochlorococcus phage P-SSP6, 17. Prochlorococcus virus PSSP7, 18. Synechococcus phage S-CBP4, 19. Synechococcus phage S-CBP42, 20. Synechococcus phage S-RIP1, 21. Cyanophage Pf-WMP3, 22. Cyanophage Pf-WMP4, 23. Synechococcus phage P60, 24. Synechococcus phage MRHenn-2013a, 25. Synechococcus phage S-SRP02, 26. Synechococcus phage S-SRP01, 27. Synechococcus phage S-SBP1, 28. Synechococcus phage S-B28, 29. Synechococcus phage S-CBP1, 30. Synechococcus phage S-CBP2, 31. Synechococcus phage S-CBP3, 32. Synechococcus phage S-RIP2, 33. Synechococcus virus Syn5, 34. Cyanophage P-TIM40, 35. Cyanophage S-RIM4, 36. Cyanophage S-RIM12, 37. Cyanophage P-RSM3, 38. Cyanophage S-RIM14, 39. Cyanophage S-RIM32, 40. Cyanophage S-RIM50, 41. Cyanophage S-SSM2, 42. Cyanophage S-SSM6a, 43. Cyanophage S-SSM6b, 44. Cyanophage S-TIM4, 45. Cyanophage S-TIM5, 46. Cyanophage Syn2, 47. Cyanophage Syn10, 48. Cyanophage Syn30, 49. Microcystis virus Ma-LMM01, 50. Prochlorococcus phage MED4-213, 51. Prochlorococcus phage P-HM1, 52. Prochlorococcus phage P-HM2, 53. Prochlorococcus phage P-RSM4, 54. Prochlorococcus phage P-SSM2, 55. Prochlorococcus phage P-SSM3, 56. Prochlorococcus phage P-SSM4, 57. Prochlorococcus phage P-SSM5, 58. Prochlorococcus phage P-SSM7, 59. Prochlorococcus phage Syn1, 60. Prochlorococcus phage Syn33, 61. Synechococcus phage KBS-M-1A, 62. Synechococcus phage S-B68, 63. Synechococcus phage S-CAM1, 64. Synechococcus phage S-CAM8, 65. Synechococcus phage S-CBM2, 66. Synechococcus phage S-IOM18, 67. Synechococcus phage S-PM2, 68. Synechococcus phage S-RIM2, 69. Synechococcus phage S-RIM8, 70. Synechococcus phage S-RSM4, 71. Synechococcus phage S-ShM2, 72. Synechococcus phage S-SKS1, 73. Synechococcus phage S-SM1, 74. Synechococcus phage S-SM2, 75. Synechococcus phage S-SSM4, 76. Synechococcus phage S-SSM5, 77. Synechococcus phage S-SSM7, 78. Synechococcus phage S-WAM1, 79. Cyanophage P-RSM1, 80. Cyanophage P-RSM6, 81. Cyanophage P-SS1, 82. Synechococcus phage S-WAM2, 83. Synechococcus phage S-CRM01, 84. Synechococcus phage BUCT-ZZ01, 85. Synechococcus phage S-H38, 86. Synechococcus phage S-SRM01, 87. Synechococcus phage S-N03, 88. Synechococcus phage S-H34, 89. Synechococcus phage B3, 90. Synechococcus phage B23, 91. Synechococcus phage S-SCSM1, 92. Synechococcus phage S-H68, 93. Synechoccus phage S-B43, 94. Synechococcus phage S-B05, 95. Synechococcus phage S-SZBM1, 96. Synechococcus phage ACG-2014f, 97. Synechococcus phage S-H9-1, 98. Synechococcus phage S-H9-2, 99. Synechococcus phage SynMITS9220M01, 100. Synechococcus phage S-H25, 101. Synechococcus phage S-P4, 102. Synechoccus phage S-E7, 103. Synechococcus phage S-B64, 104. Synechococcus phage Bellamy, 105. Synechococcus phage S-H35, 106. Synechococcus phage S-CAM9, 107. Synechococcus phage S-CAM7, 108. Synechococcus phage S-CAM4, 109. Synechococcus phage S-CAM3, 110. Synechococcus phage S-CAM22, 111. Synechococcus cyanophage S-WAM2, 112. Synechococcus cyanophage S-WAM1, 113. Synechococcus phage ACG-2014j, 114. Synechococcus phage ACG-2014i, 115. Synechococcus phage metaG-MbCM1, 116. Synechococcus phage ACG-2014g, 117. Synechococcus phage ACG-2014e, 118. Synechococcus phage ACG-2014d, 119. Synechococcus phage ACG-2014b, 120. Synechococcus phage ACG-2014a, 121. Synechococcus phage S-MbCM100, 122. Synechococcus phage S-MbCM25, 123. Synechococcus phage S-MbCM7, 124. Synechococcus phage S-RIM8 A.HR5, 125. Synechococcus phage S-RIM8 A.HR3, 126. Synechococcus phage S-RIM8 A.HR1, 127. Synechococcus phage S-RIM2 R9_2006, 128. Synechococcus phage S-RIM2 R1_1999, 129. Synechococcus phage S-RIM2 R21_2007, 130. Synechococcus phage Syn30, 131. Synechococcus phage ACG-2014c, 132. Synechococcus phage S-T4, 133. Synechococcus phage S-CBWM1, 134. Synechococcus phage ACG-2014h, 135. Synechococcus phage syn9, 136. Synechococcus phage Syn19, 137. Cyanophage KBS-S-2A, 138. Cyanophage MED4-117, 139. Cyanophage PSS2, 140. Microcystis phage MACPNOA1, 141. Prochlorococcus phage MED4-184, 142. Cyanophage S-2L, 143. Synechococcus phage S-H1, 144. Synechococcus phage S-LBS1, 145. Synechococcus phage S-CBS1, 146. Synechococcus phage S-CBS4, 147. Synechococcus phage S-CBS3, 148. Synechococcus phage S-CBS2, 149. Synechococcus virus S-ESS1, 150. Microcystis cyanophage vB_MaeS-yong1, 151. Microcystis cyanophage Mwe-Yong1112-1.
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Figure 3. Photosynthesis affected by phage infection. (A) The three multicomponent photosynthetic complexes used by cyanobacteria [43]. (B) Biosynthesis of the chromophores Phycoerythrobilin (PEB) [44], Phycocyanobilin 2, and Phytochromobilin 3. Enzymes that we note to be encoded by AMGs, through our analyses of cyanophage genomes, are depicted in an orange sphere. For the full list of cyanophages that encode HO, PcyA, PebS, and PEB, see Figure 2 entries XIII, IV, VII, and VIII, respectively. PEB could also be found in four myocyanophage genomes. (C) The polypeptide composition of and electron transport in the photosystem II complex [45]. AP: Allophycocyanin, PE: phycoerythrin, PC: phycocyanin, P680: reaction center chlorophyll a dimer, Phe: pheophytin, QA: plastoquinone tightly bound to the D2 protein, QB: exchangeable plastoquinone bound to the D1 protein, PQ: plastoquinone and PQH2: plastohydroquinone. (D) Modifications to photosynthetic pathways caused by phage infection [46]. The photosynthetic machinery and mechanisms of uninfected (top) and phage-infected cyanobacteria (bottom). Cyanophage genes involved in the photosystem are gene psbA encoding photosystem II reaction center protein D1, psbD encoding photosystem II D2 protein, ptoX encoding plastoquinol terminal oxidase (PTOX), petF encoding ferredoxin, and petE encoding plastocyanin and the NADH dehydrogenase gene (shown in red bold font). NDH, NAD(P)H dehydrogenase. COX, cytochrome c oxidase. PTOX, plastoquinol terminal oxidase. PC, plastocyanin.
Figure 3. Photosynthesis affected by phage infection. (A) The three multicomponent photosynthetic complexes used by cyanobacteria [43]. (B) Biosynthesis of the chromophores Phycoerythrobilin (PEB) [44], Phycocyanobilin 2, and Phytochromobilin 3. Enzymes that we note to be encoded by AMGs, through our analyses of cyanophage genomes, are depicted in an orange sphere. For the full list of cyanophages that encode HO, PcyA, PebS, and PEB, see Figure 2 entries XIII, IV, VII, and VIII, respectively. PEB could also be found in four myocyanophage genomes. (C) The polypeptide composition of and electron transport in the photosystem II complex [45]. AP: Allophycocyanin, PE: phycoerythrin, PC: phycocyanin, P680: reaction center chlorophyll a dimer, Phe: pheophytin, QA: plastoquinone tightly bound to the D2 protein, QB: exchangeable plastoquinone bound to the D1 protein, PQ: plastoquinone and PQH2: plastohydroquinone. (D) Modifications to photosynthetic pathways caused by phage infection [46]. The photosynthetic machinery and mechanisms of uninfected (top) and phage-infected cyanobacteria (bottom). Cyanophage genes involved in the photosystem are gene psbA encoding photosystem II reaction center protein D1, psbD encoding photosystem II D2 protein, ptoX encoding plastoquinol terminal oxidase (PTOX), petF encoding ferredoxin, and petE encoding plastocyanin and the NADH dehydrogenase gene (shown in red bold font). NDH, NAD(P)H dehydrogenase. COX, cytochrome c oxidase. PTOX, plastoquinol terminal oxidase. PC, plastocyanin.
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Table 1. Overview of cyanophage genomes.
Table 1. Overview of cyanophage genomes.
PhageTaxonomyHostGeographic DistributionGenome Length (Kb)GC%ProteinGenetRNAAccession
Anabaena phage A-4L [12]PodoviridaeAnabaenaRussia41.7543.43838 NC_024358
Cyanophage 9515-10aPodoviridaeProchlorococcusSargasso Sea, North Atlantic Ocean47.0639.25556 NC_016657
Cyanophage BHS3Podoviridae South Africa10.0354.99 MF098555
Cyanophage KBS-P-1APodoviridaeSynechococcusUSA45.7347.45758 NC_020865
Cyanophage KBS-S-1APodoviridaeSynechococcusUSA32.444.73742 JF974297
Cyanophage KBS-S-2A [13]SiphoviridaeSynechococcusAtlantic: Chesapeake Bay40.66496464 NC_020854
Cyanophage MED4-117Siphoviridae 38.8337.26363 NC_020857
Cyanophage NATL1A-7PodoviridaeProchlorococcusGulf of Aqaba, Red Sea47.7438.765691NC_016658
Cyanophage NATL2A-133Podoviridae North Pacific Ocean47.5439.96266 NC_016659
Cyanophage PP [14]PodoviridaeLeptolyngbya foveolarumChina42.4846.44141 NC_022751
Cyanophage P-RSM1Myoviridae Gulf of Aqaba, Red Sea177.2140.22122152NC_021071
Cyanophage P-RSM6Myoviridae Red Sea192.539.32212243NC_020855
Cyanophage P-SS1MyoviridaeProchlorococcusNorth Atlantic Ocean178.2836.7216217 JF974306
Cyanophage PSS2 [15]SiphoviridaeProchlorococcus 10.752.3132132 NC_013021
Cyanophage P-SSP2Podoviridae 45.8937.95659 NC_016656
Cyanophage P-TIM40MyoviridaeProchlorococcus 188.6340.72352361NC_028663
Cyanophage S-RIM4MyoviridaeSynechococcusUSA: Narragansett Bay; Rhode Island175.4641.22282379MK493321
Cyanophage S-RIM12 [16]Myoviridae USA: Narragansett Bay; Rhode Island174.339.6215 KX349318
Cyanophage S-RIM14 [17]Myoviridae USA179.541216 KX349306
Cyanophage S-RIM32 [18]Myoviridae USA194.4439.923024010NC_031235
Cyanophage S-RIM50MyoviridaeSynechococcusUSA174.3140.32272358NC_031242
Cyanophage SS120-1PodoviridaeProchlorococcusSargasso Sea, North Atlantic Ocean46.9940.552 NC_020872
Cyanophage S-SSM2MyoviridaeSynechococcusSargasso Sea179.98412072081JF974292
Cyanophage S-SSM6aMyoviridaeSynechococcusAtlantic Ocean232.88393043105HQ317391
Cyanophage S-SSM6bMyoviridaeSynechococcusSargasso Sea, North Atlantic Ocean182.3739.42182213HQ316603
Cyanophage S-TIM4Myoviridae Israel176.537.72352353NC_048015
Cyanophage S-TIM5 [10]MyoviridaeSynechococcusGulf of Aqaba, Red Sea161.4440.518019010NC_019516
Cyanophage Syn2MyoviridaeSynechococcusSargasso Sea175.641.32012096HQ634190
Cyanophage Syn10MyoviridaeSynechococcusGulf Stream17740.62052166HQ634191
Cyanophage Syn30MyoviridaeSynechococcusNE Providence Channel178.8139.92092166NC_021072
Microcystis phage MACPNOA1SiphoviridaeMicrocystis 42.4768.353 KY697807
Microcystis virus Ma-LMM01 [19]MyoviridaeMicrocystisJapan1621461841862NC_008562
Phormidium virus WMP3PodoviridaePhormidium 43.2546.54141 NC_009551
Phormidium virus WMP4 [20]PodoviridaePhormidium 40.9451.84545 NC_008367
Prochlorococcus phage MED4-184SiphoviridaeProchlorococcus 38.3337.25960 NC_020847
Prochlorococcus phage MED4-213MyoviridaeProchlorococcusNorth Pacific Ocean180.9837.8216218 NC_020845
Prochlorococcus phage P-GSP1PodoviridaeProchlorococcusGulf Stream, North Atlantic Ocean44.9539.64953 NC_020878
Prochlorococcus phage P-HM1 [21]MyoviridaeProchlorococcus 181.0437.8241241 NC_015280
Prochlorococcus phage P-HM2 [21]MyoviridaeProchlorococcus 183.8138242242 NC_015284
Prochlorococcus phage P-RSM4 [21]MyoviridaeProchlorococcusRed Sea176.4337.62392423NC_015283
Prochlorococcus phage P-RSP2PodoviridaeProchlorococcusGulf of Aqaba, Red Sea42.26344648 HQ332139
Prochlorococcus phage P-SSM2MyoviridaeProchlorococcus 252.435.5332 GU071092
Prochlorococcus phage P-SSM3MyoviridaeProchlorococcusAtlantic Ocean179.0636.7214223 NC_021559
Prochlorococcus phage P-SSM4 [21]MyoviridaeProchlorococcusSargasso Sea, North Atlantic Ocean178.2536.7221221 NC_006884.2
Prochlorococcus phage P-SSM5MyoviridaeProchlorococcusSargasso Sea, North Atlantic Ocean252.0135.53193311HQ632825
Prochlorococcus phage P-SSM7 [21]MyoviridaeProchlorococcus 1828372372414NC_015290
Prochlorococcus phage P-SSP3PodoviridaeProchlorococcusSargasso Sea, North Atlantic Ocean46.237.95356 NC_020874
Prochlorococcus phage P-SSP6PodoviridaeProchlorococcusSargasso Sea, North Atlantic Ocean47.0439.24854 HQ634152
Prochlorococcus phage Syn1 [21]MyoviridaeProchlorococcus 191.240.62342406NC_015288
Prochlorococcus phage Syn33 [21]MyoviridaeProchlorococcusGulf Stream, North Atlantic Ocean174.2939.62272325NC_015285
Prochlorococcus virus PSSP7PodoviridaeProchlorococcus 4538.858 NC_006882
Synechococcus phage KBS-M-1AMyoviridaeSynechococcusUSA171.7440.62132218JF974293
Synechococcus phage S-B68 MyoviridaeSynechococcusChina163.9851.7229 MK016664
Synechococcus phage S-CAM1MyoviridaeSynechococcusUSA197.543250 NC_020837
Synechococcus phage S-CAM8MyoviridaeSynechococcusUSA171.839.2207 NC_021530
Synechococcus phage S-CBM2MyoviridaeSynechococcusUSA180.8939.71972128HQ633061
Synechococcus phage S-CBP4PodoviridaeSynechococcus 429.844.3547 NC_048183
Synechococcus phage S-CBP42 [22]PodoviridaeSynechococcus 45.254.651 NC_029031
Synechococcus phage S-EIVl [23]unclassified bacterial virusesSynechococcusCanada79845.9 129 KJ410740
Synechococcus phage S-IOM18MyoviridaeSynechococcusArabian Sea171.840.62092197NC_021536
Synechococcus phage S-PM2 [8]MyoviridaeSynechococcusEnglish Channel191.537.8227 NC_006820
Synechococcus phage S-RIM2 [17]MyoviridaeSynechococcusUSA175.342.2214 KX349284
Synechococcus phage S-RIM8 [17]MyoviridaeSynechococcusUSA170.340.6221 NC_047733
Synechococcus phage S-RIP1PodoviridaeSynechococcusUSA44.8942.95561 NC_020867
Synechococcus phage S-RIP2PodoviridaeSynechococcusUSA45.7347.35457 NC_020838
Synechococcus phage S-RSM4 [24]MyoviridaeSynechococcusGulf of Aqaba, Red Sea194.454123724912NC_013085
Synechococcus phage S-ShM2 [21]MyoviridaeSynechococcus 179.56412302311NC_015281
Synechococcus phage S-SKS1MyoviridaeSynechococcusNorth Atlantic Ocean208.013628130211NC_020851
Synechococcus phage S-SM1 [21]MyoviridaeSynechococcus 174.08412342406NC_015282
Synechococcus phage S-SM2 [21]MyoviridaeSynechococcus 190.7940.426727811NC_015279
Synechococcus phage S-SSM4MyoviridaeSynechococcusW Sargasso Sea182.839.42202233NC_020875
Synechococcus phage S-SSM5 [21]MyoviridaeSynechococcusSargasso Sea1768402252294NC_015289
Synechococcus phage S-SSM7 [21]MyoviridaeSynechococcusSargasso Sea232.88393193245NC_015287
Synechococcus phage S-WAM1 [18]MyoviridaeSynechococcusUSA18544.72212254NC_031944
Synechococcus phage S-WAM2 [18]MyoviridaeSynechococcusUSA186.3941.322924112NC_031935
Synechococcus phage syn9 [25]MyoviridaeSynechococcusNorth Atlantic Ocean177.340.62262326NC_008296.2
Synechococcus phage Syn19 [21]MyoviridaeSynechococcusSargasso Sea, North Atlantic Ocean175.2341.32152216NC_015286
Plectonema phage JingP1unclassified bacterial virusesPlectonemaChina40.852.349 ON677538
Cyanophage Pf-WMP3 [26]PodoviridaePhormidium 43.2546.54141 NC_009551
Cyanophage Pf-WMP4 [20]PodoviridaePhormidium 40.9451.84545 NC_008367
Synechococcus phage P60 [27]PodoviridaeSynechococcus 46.6853.35555 NC_003390
Microcystis cyanophage Mwe-Yong1112-1SiphoviridaeMicrocystis 39.6866.653 MZ436628
Microcystis cyanophage vB_MaeS-yong1SiphoviridaeMicrocystisChina43.6766.756 MT855965
Cyanophage P-RSM3MyoviridaeProchlorococcusGulf of Aqaba, Red Sea178.7536.72082113HQ634176
Cyanophage S-2LSiphoviridae 45.08769.2556 MW334946
Synechococcus phage MRHenn-2013aPodoviridaeSynechococcusChesapeake Bay92.4751.21301311JF974303
Synechococcus phage S-SRP02 [28]PodoviridaeSynechococcusSingapore42463.44747 MW822601
Synechococcus phage S-SRP01PodoviridaeSynechococcusSingapore45.0248.956 MW015080
Synechococcus phage S-SBP1PodoviridaeSynechococcusChina45.5246.85555 MT424636
Synechococcus phage S-B28PodoviridaeSynechococcusChina45.42425555 NC_048171
Synechococcus phage S-CBP1 [22]PodoviridaeSynechococcus 46.5547.65051 NC_025456
Synechococcus phage S-CBP2 [22]PodoviridaeSynechococcus 46.24555353 NC_025455
Synechococcus phage S-CBP3PodoviridaeSynechococcus 46.6246.8554 NC_025461
Synechococcus phage S-CRM01 [29]MyoviridaeSynechococcusUSA178.5639.729729733NC_015569
Synechococcus phage BUCT-ZZ01MyoviridaeSynechococcusChina156.0638.5236 ON615601
Synechococcus phage S-H38 MyoviridaeSynechococcusChina180.2242.4211 8NC_070964
Synechococcus phage S-SRM01MyoviridaeSynechococcusSingapore240.8435.6353 NC_070963
Synechococcus phage S-N03MyoviridaeSynechococcusChina167.0750247 1NC_070959
Synechococcus phage S-H34MyoviridaeSynechococcusChina167.0450246 5NC_070965
Synechococcus phage B3 [30]MyoviridaeSynechococcusCanada244.9335.437039121MN695334
Synechococcus phage B23 [30]MyoviridaeSynechococcusCanada243.6335.436538419MN695335
Synechococcus phage S-SCSM1MyoviridaeSynechococcusChina228.8336.82822939NC_070962
Synechococcus phage S-H68MyoviridaeSynechococcusChina79.6440.4117 MK016663
Synechoccus phage S-B43MyoviridaeSynechococcusChina21439.4262 MN018232
Synechococcus phage S-B05MyoviridaeSynechococcusChina20939.9268 MK799832
Synechococcus phage S-SZBM1Myoviridae;SynechococcusChina177.8343.32182186NC_070839
Synechococcus phage ACG-2014fMyoviridae;Synechococcus 222.341.6286 NC_026927
Synechococcus phage S-H9-1Myoviridae;SynechococcusChina192.4541229 12NC_070961
Synechococcus phage S-H9-2Myoviridae;SynechococcusChina187.3240.3202 14NC_070960
Synechococcus phage SynMITS9220M01Myoviridae;SynechococcusPacific Ocean190.2438.824525510MT408532
Synechococcus phage S-H25Myoviridae;SynechococcusChina190.2438.824525510MT162468
Synechococcus phage S-P4Myoviridae;SynechococcusChina158.4839.9195195 NC_048102
Synechoccus phage S-E7Myoviridae;SynechococcusChina177.6239.9216 MH920640
Synechococcus phage S-B64 Myoviridae;SynechococcusChina151.8741.3156156 NC_055744
Synechococcus phage BellamyMyoviridae;SynechococcusUSA204.934126927910NC_047838
Synechococcus phage S-H35Myoviridae;SynechococcusChina174.2341.2204204 NC_055719
Synechococcus phage S-CAM9 [18]Myoviridae;SynechococcusUSA174.839228 NC_031922
Synechococcus phage S-CAM7 [18]Myoviridae;SynechococcusUSA215.241.2266 NC_031927
Synechococcus phage S-CAM4 [18]Myoviridae; SynechococcusUSA19238.6238 NC_031900
Synechococcus phage S-CAM3 [18]Myoviridae;SynechococcusUSA197.841.6239 NC_031906
Synechococcus phage S-CAM22 [18]Myoviridae;SynechococcusUSA; Mexico172.339.9214 NC_055710
Synechococcus cyanophage S-WAM2Myoviridae;SynechococcusUSA186.3941.322924112NC_031935
Synechococcus cyanophage S-WAM1Myoviridae;SynechococcusUSA18544.72212254NC_031944
Synechococcus phage ACG-2014jMyoviridae;Synechococcus 19238.65226 NC_062735
Synechococcus phage ACG-2014iMyoviridaeSynechococcus 190.7739212212 NC_027132
Synechococcus phage metaG-MbCM1MyoviridaeSynechococcus 172.8839.8234234 NC_019443
Synechococcus phage ACG-2014gMyoviridaeSynechococcus 174.8939.3216216 NC_026924
Synechococcus phage ACG-2014eMyoviridaeSynechococcus 189.438.9214 NC_062736
Synechococcus phage ACG-2014dMyoviridaeSynechococcus 179.240.3218 NC_062734
Synechococcus phage ACG-2014bMyoviridaeSynechococcus 172.739216 NC_047719
Synechococcus phage ACG-2014aMyoviridaeSynechococcus 171.339.4208 KJ019163
Synechococcus phage S-MbCM100 [31]MyoviridaeSynechococcusUSA170.4439.4210210 NC_023584
Synechococcus phage S-MbCM25 [31]MyoviridaeSynechococcusUSA176.0439215215 KF156339
Synechococcus phage S-MbCM7 [31]MyoviridaeSynechococcusUSA189.3140.5221221 NC_023587
Synechococcus phage S-RIM8 A.HR5 [32]MyoviridaeSynechococcusNorth Atlantic Ocean168.3340.62032118HQ317385
Synechococcus phage S-RIM8 A.HR3 [32]MyoviridaeSynechococcusNorth Atlantic Ocean171.2140.62122208JF974289
Synechococcus phage S-RIM8 A.HR1 [32]MyoviridaeSynechococcusNorth Atlantic Ocean171.2140.62112198NC_020486
Synechococcus phage S-RIM2 R9_2006MyoviridaeSynechococcusNorth Atlantic Ocean175.4242.22102176HQ317291
Synechococcus phage S-RIM2 R1_1999MyoviridaeSynechococcusNorth Atlantic Ocean175.4342.22102166NC_020859
Synechococcus phage S-RIM2 R21_2007MyoviridaeSynechococcusNorth Atlantic Ocean175.4342.22082146HQ317290
Synechococcus phage Syn30MyoviridaeSynechococcusNE Providence Channel178.8139.92092166NC_021072
Synechococcus phage ACG-2014cMyoviridaeSynechococcus 17639215 NC_019444
Synechococcus phage ACG-2014hMyoviridaeSynechococcusUSA189.3140.5221221 NC_023587
Synechococcus phage S-H1SiphoviridaeSynechococcusChina39653.764 MK105819
Synechococcus phage S-LBS1SiphoviridaeSynechococcusFrance34.6460.25252 MG271909
Synechococcus phage S-CBS1 [9]SiphoviridaeSynechococcusUSA30.3358.74343 NC_016164
Synechococcus phage S-CBS4 [9]SiphoviridaeSynechococcus 69.450.8105 NC_016766
Synechococcus phage S-CBS3 [9]SiphoviridaeSynechococcus 3360.74646 NC_015465
Synechococcus phage S-CBS2 [9]SiphoviridaeSynechococcus 72.3354.5102102 NC_015463
Synechococcus phage S-T4MyoviridaeSynechococcusChina181.0838.9226226 NC_048049
Synechococcus phage S-CBWM1 [33]MyoviridaeSynechococcusUSA139.0751.617821636NC_048106
Synechococcus virus S-ESS1SiphoviridaeSynechococcusChina60.3660.95252 NC_052968
Synechococcus virus Syn5 [34]PodoviridaeSynechococcus 46.21556161 NC_009531
Table 2. AMGs in the sequenced cyanophage genomes.
Table 2. AMGs in the sequenced cyanophage genomes.
Protein FunctionProteinRatiosProtein FunctionProteinRatios
Photosynthesisphotosystem II reaction center protein PsbA/D1111/153MethylationDNA methylase23/153
photosystem II D2 protein77/153 DNA adenine methylase Dam55/153
phycocyanobilin:ferredoxin oxidoreductase (PcyA)16/153 cytosine methyltransferase11/153
ferredoxin (PetF)44/153 type II N-methyl DNA methyltransferase (group alpha)13/153
phycobilin lyase (CpeT)31/153 phage host specificity protein3/153
phycoerythrobilin synthase (PebS)9/153
phycoerythrobilin4/153Phosphate metabolismphosphate-starvation-inducible protein (PhoH)101/153
plastoquinol terminal oxidase PtoX35/153 phosphate-induced stress protein2/153
plastocyanin (PetE)70/153 phosphate ABC transporter phosphate-binding protein PstS31/153
heme oxygenase (Ho)8/153
hli gene family/high-light inducible protein90/153
thioredoxin5/153Regulatory factorCobS/cobO/cobA39/153
rfaE bifunctional protein1/153 RNA polymerase sigma factor RpoD/RpoS65/153
2OG-Fe(II) oxygenase family oxidoreductase76/153 ERF family protein1/153
NADH dehydrogenase subunit I1/153 MazG64/153
NAD synthetase24/153 RegA photosynthetic apparatus regulatory protein48/153
Carbon metabolismcarboxylesterase24/153 transcriptional regulator7/153
FAD-dependent thymidylate synthase/thymidylate synthase95/153 late promoter transcriptional accessory protein41/153
transaldolase family protein TalC in this family96/153 CP12 carbon metabolic regulator31/153
glucose 6-phosphate dehydrogenase Zwf25/153 MarR family protein3/153
6-phosphogluconate dehydrogenase Gnd22/153 translational regulator3/153
UDP-glucose 6-dehydrogenase1/153 XRE family transcriptional regulator1/153
acyl carrier protein ACP2/153 Nrd48/153
fructose-6-phosphate aldolase1/153 tryptophan halogenase20/153
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Wang, Y.; Ferrinho, S.; Connaris, H.; Goss, R.J.M. The Impact of Viral Infection on the Chemistries of the Earth’s Most Abundant Photosynthesizes: Metabolically Talented Aquatic Cyanobacteria. Biomolecules 2023, 13, 1218. https://0-doi-org.brum.beds.ac.uk/10.3390/biom13081218

AMA Style

Wang Y, Ferrinho S, Connaris H, Goss RJM. The Impact of Viral Infection on the Chemistries of the Earth’s Most Abundant Photosynthesizes: Metabolically Talented Aquatic Cyanobacteria. Biomolecules. 2023; 13(8):1218. https://0-doi-org.brum.beds.ac.uk/10.3390/biom13081218

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Wang, Yunpeng, Scarlet Ferrinho, Helen Connaris, and Rebecca J. M. Goss. 2023. "The Impact of Viral Infection on the Chemistries of the Earth’s Most Abundant Photosynthesizes: Metabolically Talented Aquatic Cyanobacteria" Biomolecules 13, no. 8: 1218. https://0-doi-org.brum.beds.ac.uk/10.3390/biom13081218

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