Genetic Mapping and Analysis of a Compact Plant Architecture and Precocious Mutant in Upland Cotton
, Guangling Shui 1, Nurimanguli Aini 1, Binghui Tang 4, Chunping Guo 1, Peng Han 1, Panxia Shao 1, Xiaomin Tian 1, Xinyi Chang 1, Qiushuang An 1, Chunmei Ma 1, Chunyuan You 3,4,*, Longfu Zhu 1,3,* and Xinhui Nie 1,*Round 1
Reviewer 1 Report
In the Manuscript “Genetic mapping and analysis of a compact plant architecture and precocious mutant in Upland cotton” Phenotypic analysis showed that the type of fruit branches in cotton is controlled by a single recessive gene. Using a F2 population crossed by Huaxin102 (normal branch) and 04N-11 (nulliplex-branch), BSA (Bulked Segregant Analysis) resequencing analysis and GhNB gene cloning in 04N-11 and allelic test showed that fruit branch type was controlled by GhNB gene located on chromosome D07. Ghnb5, a new recessive genotype of GhNB, was found in 04N-11. Through candidate gene association analysis, a SNP 20_15811516_SNV was found to be associated with plant architecture and early-maturity in xinjiang natural population.
The Ms is nicely designed and executed.
It can be accepted for publication after minor revision-
- in the Xinjiang natural population.
- 103; A total of 248 cotton v
- The Shihezi Academy of Agricultural Sciences
- Results
I could see several typos, missing articles, missing punctuations etc.
Author Response
Comment 1: It can be accepted for publication after minor revision-
- in the Xinjiang natural population.
- 103: A total of 248 cotton v
- The Shihezi Academy of Agricultural Sciences
- Results
Response: Thank you for your comments and suggestions. We have revised the non-standard parts of the full text in strict accordance with your suggestions to make the article more natural to read. Modifications have been marked in red in the manuscript.
Comment 2: I could see several typos, missing articles, missing punctuations etc.
Response: Thank you for your comments and suggestions. We have corrected the non-standard punctuation, writing and quotation in the article. Modifications have been marked in red or added annotation in the manuscript.
Reviewer 2 Report
Summary
Harvesting cotton by hand is the highest cost of cotton plant. Mechanization of harvesting can improve cotton production efficiency, reduce the cost of labor and the production cost.
Compact plant architecture, flocculation concentration and defoliant sensitive varieties are more suitable for mechanized harvesting.
Plant architecture is an important agronomic factor influencing various traits such as yield and variety adaptability in crop plants and is a combination of various components such as branching pattern, growth habit, phyllotaxis, branching angle, and so on. Due to the complexity of plant architecture, genetic based analysis is necessary.
In this article, authors presented how they identified, cloned, and mapped a recessive nulliplex-branch gene (Ghnb5) candidate interval on chromosome D07 by using Bulked Segregant Analysis (BSA) based on a new mapping population Huaxin102 (normal branch) x 04N-11 20 (nulliplex-branch).
Comments
1 The introduction session provides relatively sufficient information, except it would be better to add something saying that what is new in this manuscript. For example, is there any published articles done by other research group(s) on similar project? If yes, provides a list of them and explain what is new in this work by comparing with those published articles (such as a new mapping population was created and used in this study).
2. Line 107-112: the sub-title is “Identification and genetic analysis of fruit branch types” - it is not clear what “genetic analysis” method used here, the whole paragraph seems talking about identification …
3. Line 187-200 and 276-282: would authors willing to share genotype data?
Author Response
Comment 1: The introduction session provides relatively sufficient information. Except it will be better to add something saying that what is new in this manuscript. For example is there any published articles done by other research groups on similar project? If yes provide a list of them and explain what is new in this work by comparing whit those published articles (such as a new mapping population was created and used in this study).
Response: Thank you for your comments and suggestions. We have added a paragraph in the introduction to clarify our main research content. Modifications have been marked in red in the manuscript.
Comment 2: Line 107-122: the sub-title is “Identification and genetic analysis of fruit branch types” - it is not clear what “genetic analysis” method used here. The whole paragraph seems talking about indentification...
Response: Thank you for your comments and suggestions. We have removed the “genetic analysis” to make sure the sub-title more consistent with the description below. The Modification has been annotated.
Comment 3: Line 187-200 and 276-282: would authors willing to share genotype data?
Response: Thank you for your comments and suggestions. We are very willing to share genotypic data, and we have added the genotypic data related to line276-282 in the Table S2. Meanwhile, we have uploaded the resequencing data of Huaxin102 and 04n-11 to NCBI, the ID of sequencing data is PRJNA841236. Modifications have been marked in red in the manuscript.
