Effect of Zearalenone and Hormone Regulators on Microspore Embryogenesis in Anther Culture of Wheat
Abstract
:1. Introduction
2. Results
2.1. Influence of ZEN on Androgenic Structures Formation
2.2. Influence of ZEN on Green Plant Regeneration
2.3. Influence of ZEN on Albino Plant Regeneration
2.4. Influence of ZEN on Spontaneous Doubled Haploid Formation
3. Discussion
4. Materials and Methods
4.1. Plant Material and Growth Condition
4.2. In Vitro Culture Conditions
4.3. Data Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Genotype | No. of Placed Anthers | Treatment | ||||||
---|---|---|---|---|---|---|---|---|
D0 | D1 | D2 | K0 | K1 | K2 | |||
W1 | Century x Wichita | 1800 | 1,00 | 1,33 | 6,33 | 3,00 | 9,00 | 4,00 |
W2 | Augusta x Tam 107 | 1800 | 0,00 | 3,00 | 3,00 | 1,67 | 1,33 | 3,33 |
W3 | Freedom x Ok 101 | 1800 | 5,00 | 2,67 | 3,00 | 0,33 | 1,67 | 1,33 |
W4 | Antelope x Lr 1 | 1800 | 9,33 | 11,00 | 15,33 | 7,33 | 25,67 | 13,33 |
W5 | Geneva x Century | 1800 | 1,33 | 4,00 | 4,67 | 1,67 | 1,00 | 3,67 |
W6 | Clark x Choptank | 1800 | 3,33 | 3,67 | 9,67 | 4,67 | 2,33 | 4,00 |
W7 | Klasic x Clark | 1800 | 1,00 | 0,67 | 2,33 | 0,00 | 1,00 | 1,67 |
W8 | Thatcher x Chińska | 1800 | 2,00 | 18,33 | 21,67 | 1,67 | 11,00 | 10,33 |
W9 | GSTR 420 x Choptank | 1800 | 1,00 | 12,67 | 14,00 | 0,33 | 7,67 | 12,33 |
W10 | Wichita x KS96WGRC36 | 1800 | 1,33 | 3,33 | 2,33 | 1,00 | 2,67 | 1,33 |
W11 | Tam 107 x Clark | 1800 | 0,33 | 0,33 | 0,33 | 0,00 | 0,00 | 0,00 |
W12 | Karl 92 x Choptank | 1800 | 0,67 | 1,67 | 1,33 | 0,67 | 0,33 | 2,00 |
W13 | KS96WGRC36 x NC8889-2A | 1800 | 5,67 | 17,67 | 15,33 | 11,33 | 19,33 | 12,33 |
S1 | Arabella x T71 | 1800 | 18,00 | 12,67 | 8,67 | 11,00 | 3,67 | 1,00 |
S2 | Tybalt x T71 | 1800 | 13,33 | 6,33 | 13,67 | 6,33 | 5,67 | 17,67 |
S3 | Mandaryna x T71 | 1800 | 7,67 | 9,00 | 10,67 | 0,00 | 2,67 | 2,00 |
S4 | Toridon x T71 | 1800 | 9,33 | 7,33 | 8,67 | 8,67 | 9,00 | 8,67 |
S5 | T68xDC356/08-4-5/09 | 1800 | 14,00 | 10,67 | 9,00 | 2,67 | 3,00 | 10,67 |
S6 | T71xDC356/08-4-5/09 | 1800 | 6,00 | 17,67 | 17,67 | 15,00 | 12,00 | 18,00 |
Genotype | No. of Placed Anthers | Treatment | ||||||
---|---|---|---|---|---|---|---|---|
D0 | D1 | D2 | K0 | K1 | K2 | |||
W1 | Century x Wichita | 1800 | 0,33 | 0,67 | 0,00 | 1,33 | 3,00 | 1,67 |
W2 | Augusta x Tam 107 | 1800 | 0,00 | 0,33 | 0,33 | 0,33 | 0,67 | 1,33 |
W3 | Freedom x Ok 101 | 1800 | 0,00 | 0,00 | 1,00 | 0,00 | 0,33 | 1,00 |
W4 | Antelope x Lr 1 | 1800 | 0,00 | 2,00 | 3,33 | 1,33 | 8,00 | 6,00 |
W5 | Geneva x Century | 1800 | 0,33 | 0,67 | 0,33 | 0,33 | 0,33 | 0,67 |
W6 | Clark x Choptank | 1800 | 3,00 | 1,00 | 4,67 | 2,33 | 1,33 | 3,00 |
W7 | Klasic x Clark | 1800 | 0,33 | 0,33 | 1,00 | 0,00 | 0,33 | 0,33 |
W8 | Thatcher x Chińska | 1800 | 0,67 | 9,33 | 10,67 | 1,67 | 7,33 | 5,33 |
W9 | GSTR 420 x Choptank | 1800 | 0,33 | 7,67 | 6,00 | 0,00 | 4,00 | 8,00 |
W10 | Wichita x KS96WGRC36 | 1800 | 0,00 | 2,33 | 0,67 | 0,33 | 0,67 | 1,00 |
W11 | Tam 107 x Clark | 1800 | 0,00 | 0,00 | 0,00 | 0,00 | 0,00 | 0,00 |
W12 | Karl 92 x Choptank | 1800 | 0,00 | 0,33 | 0,67 | 0,00 | 0,00 | 0,00 |
W13 | KS96WGRC36 x NC8889-2A | 1800 | 2,67 | 7,67 | 7,67 | 1,33 | 6,00 | 9,33 |
S1 | Arabella x T71 | 1800 | 8,67 | 6,67 | 5,33 | 4,33 | 2,33 | 0,00 |
S2 | Tybalt x T71 | 1800 | 7,67 | 4,33 | 4,67 | 5,00 | 2,33 | 11,00 |
S3 | Mandaryna x T71 | 1800 | 5,67 | 7,33 | 9,67 | 0,00 | 1,33 | 1,33 |
S4 | Toridon x T71 | 1800 | 7,00 | 6,00 | 7,67 | 6,33 | 6,00 | 6,67 |
S5 | T68xDC356/08-4-5/09 | 1800 | 7,00 | 4,00 | 3,00 | 3,33 | 4,33 | 4,00 |
S6 | T71xDC356/08-4-5/09 | 1800 | 5,67 | 8,00 | 7,00 | 13,67 | 6,33 | 12,67 |
Genotype | No. of Placed Anthers | Treatment | ||||||
---|---|---|---|---|---|---|---|---|
D0 | D1 | D2 | K0 | K1 | K2 | |||
W1 | Century x Wichita | 1800 | 0,33 | 0,00 | 1,67 | 1,00 | 2,33 | 0,33 |
W2 | Augusta x Tam 107 | 1800 | 0,00 | 0,33 | 0,33 | 0,00 | 0,67 | 0,67 |
W3 | Freedom x Ok 101 | 1800 | 1,33 | 0,67 | 1,33 | 0,33 | 0,67 | 0,00 |
W4 | Antelope x Lr 1 | 1800 | 3,00 | 3,00 | 4,67 | 3,00 | 5,00 | 2,33 |
W5 | Geneva x Century | 1800 | 0,33 | 0,67 | 1,67 | 0,00 | 0,33 | 2,00 |
W6 | Clark x Choptank | 1800 | 0,00 | 1,33 | 0,67 | 1,67 | 0,33 | 0,33 |
W7 | Klasic x Clark | 1800 | 0,33 | 0,67 | 0,33 | 0,33 | 0,00 | 0,00 |
W8 | Thatcher x Chińska | 1800 | 0,00 | 2,33 | 1,67 | 0,00 | 0,67 | 1,33 |
W9 | GSTR 420 x Choptank | 1800 | 0,00 | 0,67 | 0,00 | 0,00 | 1,00 | 1,00 |
W10 | Wichita x KS96WGRC36 | 1800 | 0,00 | 0,00 | 0,00 | 0,00 | 0,00 | 0,00 |
W11 | Tam 107 x Clark | 1800 | 0,00 | 0,00 | 0,00 | 0,00 | 0,00 | 0,00 |
W12 | Karl 92 x Choptank | 1800 | 0,00 | 0,00 | 0,00 | 0,00 | 0,00 | 0,00 |
W13 | KS96WGRC36 x NC8889-2A | 1800 | 1,33 | 1,67 | 1,33 | 0,33 | 1,00 | 0,00 |
S1 | Arabella x T71 | 1800 | 4,67 | 1,33 | 0,67 | 0,00 | 0,00 | 0,00 |
S2 | Tybalt x T71 | 1800 | 1,00 | 0,67 | 0,00 | 0,00 | 0,00 | 0,33 |
S3 | Mandaryna x T71 | 1800 | 1,33 | 1,67 | 1,00 | 0,00 | 0,33 | 0,33 |
S4 | Toridon x T71 | 1800 | 1,67 | 0,00 | 0,33 | 0,67 | 0,67 | 0,00 |
S5 | T68xDC356/08-4-5/09 | 1800 | 8,00 | 4,67 | 7,33 | 0,00 | 0,33 | 1,00 |
S6 | T71xDC356/08-4-5/09 | 1800 | 4,33 | 5,00 | 5,33 | 3,33 | 1,33 | 1,00 |
Component (mL/L) | Treatment | |||||
---|---|---|---|---|---|---|
D0 | D1 | D2 | K0 | K1 | K2 | |
ZEN | - | 0.1 | 0.2 | - | 0.1 | 0.2 |
2,4-D | 1.0 | 1.0 | 1.0 | 1.5 | 1.5 | 1.5 |
Dicamba | 1.0 | 1.0 | 1.0 | - | - | - |
Kinetin | - | - | - | 0.5 | 0.5 | 0.5 |
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Weigt, D.; Niemann, J.; Siatkowski, I.; Zyprych-Walczak, J.; Olejnik, P.; Kurasiak-Popowska, D. Effect of Zearalenone and Hormone Regulators on Microspore Embryogenesis in Anther Culture of Wheat. Plants 2019, 8, 487. https://0-doi-org.brum.beds.ac.uk/10.3390/plants8110487
Weigt D, Niemann J, Siatkowski I, Zyprych-Walczak J, Olejnik P, Kurasiak-Popowska D. Effect of Zearalenone and Hormone Regulators on Microspore Embryogenesis in Anther Culture of Wheat. Plants. 2019; 8(11):487. https://0-doi-org.brum.beds.ac.uk/10.3390/plants8110487
Chicago/Turabian StyleWeigt, Dorota, Janetta Niemann, Idzi Siatkowski, Joanna Zyprych-Walczak, Przemysław Olejnik, and Danuta Kurasiak-Popowska. 2019. "Effect of Zearalenone and Hormone Regulators on Microspore Embryogenesis in Anther Culture of Wheat" Plants 8, no. 11: 487. https://0-doi-org.brum.beds.ac.uk/10.3390/plants8110487