An AIEE Active Anthracene-Based Nanoprobe for Zn2+ and Tyrosine Detection Validated by Bioimaging Studies
Abstract
:1. Introduction
2. Materials and Methods
2.1. Synthesis of AT2 [3,15]
2.2. Computational Methods
2.3. MTT Assay
2.4. Procedure for Live Cell and Zebra Fish Imaging of AT2 [15]
2.4.1. Cellular Imaging of AIEE
2.4.2. Cellular Imaging of Zn2+ and Tyrosine
2.4.3. Zebrafish Imaging Studies
3. Results and Discussion
3.1. Synthesis and Characterization of AT2
3.2. AIEE of AT2
3.3. Optimum Conditions for AT2-Based Sensors
3.4. Primary Sensory Results of AT2
3.5. Interference Studies and Detection Limits
3.6. TRPL, pH Effect and Stoichiometry
3.7. Association Constants and Nano-Morphological Interrogations
3.8. 1H-NMR Titrations and DFT Optimizations on Sensors
3.9. MTT Assay and Bioimaging
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Composition | HOMO (eV) | LUMO (eV) | Band Gap (eV) | λabs (nm) | λem (nm) | Φf | τ (ns) |
---|---|---|---|---|---|---|---|
Probe AT2 | −6.02 | −2.91 | 3.11 | 396 | 456 | 0.008 a | 0.1001 |
AT2@60% fW | ND | ND | ND | 407 | 470 | 0.142 a | 0.2021 |
AT2 + Zn2+ | −5.84 b | −3.01 b | 2.83 b | 401 | 520 | 0.103 a | 0.2329 |
−5.69 c | −2.64 c | 3.05c | |||||
AT2 + Zn2+ + Tyr | ND | ND | ND | 396 | 520 | 0.011 a | 0.1003 |
Tyr + Zn2+ | −5.47 d | −2.70 d | 2.77d | ND | ND | ND | ND |
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Shellaiah, M.; Thirumalaivasan, N.; Aazaad, B.; Awasthi, K.; Sun, K.W.; Wu, S.-P.; Lin, M.-C.; Ohta, N. An AIEE Active Anthracene-Based Nanoprobe for Zn2+ and Tyrosine Detection Validated by Bioimaging Studies. Chemosensors 2022, 10, 381. https://0-doi-org.brum.beds.ac.uk/10.3390/chemosensors10100381
Shellaiah M, Thirumalaivasan N, Aazaad B, Awasthi K, Sun KW, Wu S-P, Lin M-C, Ohta N. An AIEE Active Anthracene-Based Nanoprobe for Zn2+ and Tyrosine Detection Validated by Bioimaging Studies. Chemosensors. 2022; 10(10):381. https://0-doi-org.brum.beds.ac.uk/10.3390/chemosensors10100381
Chicago/Turabian StyleShellaiah, Muthaiah, Natesan Thirumalaivasan, Basheer Aazaad, Kamlesh Awasthi, Kien Wen Sun, Shu-Pao Wu, Ming-Chang Lin, and Nobuhiro Ohta. 2022. "An AIEE Active Anthracene-Based Nanoprobe for Zn2+ and Tyrosine Detection Validated by Bioimaging Studies" Chemosensors 10, no. 10: 381. https://0-doi-org.brum.beds.ac.uk/10.3390/chemosensors10100381