Impact of Oral Administration of Lactiplantibacillus plantarum Strain CNCM I−4459 on Obesity Induced by High-Fat Diet in Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strain and Growth Conditions
2.2. Animal and Experimental Design
2.3. Oral Glucose Tolerance Test (OGTT)
2.4. Measurement of Plasma Parameters
2.5. Gene and Protein Expression Analysis
2.6. Microbial DNA Extraction and Amplification
2.7. Microbiota Composition Analysis
2.8. Measurement of Short-Chain Fatty Acids (SCFAs)
2.9. Statistical Analysis
3. Results
3.1. L. plantarum CNCM I−4459 Enhanced oral Glucose Tolerance by Inhibiting Glucose Metabolism
3.2. Treatment with L. plantarum CNCM I−4459 Decreased Concentrations of Circulating Lipids and Modulated Hepatic Lipid Metabolism
3.3. Treatment with L. plantarum CNCM I—4459 Reduce ileal Inflammation Affect Moderately Epithelial Junctions
3.4. L. plantarum CNCM I −4459 Treatment Partially Reversed the Effect of Diet on Gut Microbiota Composition
3.5. L. plantarum CNCM I−4459 Did Not Affect Fecal End-Products of Fermentation
3.6. Correlation between Microbiota Composition at Family-Level and Physiological Parameters
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Diet | HFD–PBS | CD–PBS | HFD—L. plantarum CNCM I−4459 |
---|---|---|---|
TG (mmol/L) | 1.21 ± 0.24 | 0.94 ± 0.15 | 1.17 ± 0.28 |
LDL-c (mmol/L) | 0.42 ± 0.06 | 0.24 ± 0.04 **** | 0.26 ± 0.04 **** |
HDL-c (mmol/L) | 2.67 ± 0.18 | 2.15 ± 0.17 * | 2.76 ± 0.55 |
FFA (mmol/L) | 1.3 ± 0.12 | 1.01 ± 0.38 | 1.09 ± 0.21 |
TC (mmol/L) | 4.85 ± 0.43 | 3.64 ± 0.25 ** | 4.77 ± 0.94 |
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Jacouton, E.; Mondot, S.; Langella, P.; Bermúdez-Humarán, L.G. Impact of Oral Administration of Lactiplantibacillus plantarum Strain CNCM I−4459 on Obesity Induced by High-Fat Diet in Mice. Bioengineering 2023, 10, 1151. https://0-doi-org.brum.beds.ac.uk/10.3390/bioengineering10101151
Jacouton E, Mondot S, Langella P, Bermúdez-Humarán LG. Impact of Oral Administration of Lactiplantibacillus plantarum Strain CNCM I−4459 on Obesity Induced by High-Fat Diet in Mice. Bioengineering. 2023; 10(10):1151. https://0-doi-org.brum.beds.ac.uk/10.3390/bioengineering10101151
Chicago/Turabian StyleJacouton, Elsa, Stanislas Mondot, Philippe Langella, and Luis G. Bermúdez-Humarán. 2023. "Impact of Oral Administration of Lactiplantibacillus plantarum Strain CNCM I−4459 on Obesity Induced by High-Fat Diet in Mice" Bioengineering 10, no. 10: 1151. https://0-doi-org.brum.beds.ac.uk/10.3390/bioengineering10101151