Normothermic Ex Vivo Liver Platform Using Porcine Slaughterhouse Livers for Disease Modeling
Abstract
:1. Introduction
2. Materials and Methods
2.1. Liver and Blood Procurement
2.2. Circuit Design
2.3. Reperfusion
2.4. Free Fatty Acid Treatment
2.5. Acetaminophen Treatment
2.6. Analysis
2.7. Gene Expression
2.8. Histology
2.9. Indocyanine Green Functionality Assay
2.10. Data Analysis
3. Results
3.1. Untreated Livers
3.2. Free Fatty Acid Treatment
3.3. Acetaminophen Treatment
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Krüger, M.; Ruppelt, A.; Kappler, B.; Van Soest, E.; Samsom, R.A.; Grinwis, G.C.M.; Geijsen, N.; Helms, J.B.; Stijnen, M.; Kock, L.M.; et al. Normothermic Ex Vivo Liver Platform Using Porcine Slaughterhouse Livers for Disease Modeling. Bioengineering 2022, 9, 471. https://0-doi-org.brum.beds.ac.uk/10.3390/bioengineering9090471
Krüger M, Ruppelt A, Kappler B, Van Soest E, Samsom RA, Grinwis GCM, Geijsen N, Helms JB, Stijnen M, Kock LM, et al. Normothermic Ex Vivo Liver Platform Using Porcine Slaughterhouse Livers for Disease Modeling. Bioengineering. 2022; 9(9):471. https://0-doi-org.brum.beds.ac.uk/10.3390/bioengineering9090471
Chicago/Turabian StyleKrüger, Melanie, Alicia Ruppelt, Benjamin Kappler, Elke Van Soest, Roos Anne Samsom, Guy C. M. Grinwis, Niels Geijsen, J. Bernd Helms, Marco Stijnen, Linda M. Kock, and et al. 2022. "Normothermic Ex Vivo Liver Platform Using Porcine Slaughterhouse Livers for Disease Modeling" Bioengineering 9, no. 9: 471. https://0-doi-org.brum.beds.ac.uk/10.3390/bioengineering9090471