Role of Cytosolic Malic Enzyme in Oleaginicity of High-Lipid-Producing Fungal Strain Mucor circinelloides WJ11
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains, Growth, and Transformation Conditions
2.2. Plasmid Construction
2.3. Preparation of Genomic DNA
2.4. Reverse Transcription-Quantitative PCR (RT-qPCR) to Analyse the Expression of Genes
2.5. Measurement of the Concentration of Nitrogen and Glucose in Culture Medium
2.6. Analysis of CDW and Lipid Accumulation
2.7. Determination of ME Activity
2.8. Statistical Analysis
3. Results
3.1. Genetic Engineering for Generation of cmalA and cmalB Overexpressing M. circinelloides Strains
3.2. The Expression Levels of cmalA and cmalB Genes in Overexpressing Strains
3.3. ME Activity Analysis of cmalA and cmalB Overexpressing Strains
3.4. Cell Growth and Lipid Accumulation in cmalA and cmalB Overexpressing Strains
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Strains | Time (h) | 16:0 | 16:1 | 18:0 | 18:1 | 18:2 (LA) | 18:3 (GLA) |
---|---|---|---|---|---|---|---|
Mc-cmal-2075 | 24 | 17.44 ± 0.3 | 2.61 ± 0.2 | 4.90 ± 0.1 | 39.10 ± 0.9 | 11.01 ± 0.8 | 13.59 ± 0.4 |
48 | 20.18 ± 0.5 | 2.92 ± 0.2 | 5.18 ± 0.3 | 41.12 ± 1.0 | 10.74 ± 0.6 | 10.80 ± 0.2 | |
72 | 22.55 ± 0.7 | 3.41 ± 0.1 | 4.62 ± 0.3 | 41.88 ± 2.1 | 11.09 ± 0.6 | 9.76 ± 0.3 | |
96 | 22.59 ± 0.5 | 4.00 ± 0.3 | 3.93 ± 0.1 | 42.94 ± 1.5 | 12.41 ± 0.3 | 9.91 ± 05 | |
Mc-cmalA-4 | 24 | 17.96 ± 0.4 * | 1.92 ± 0.2 * | 4.89 ± 0.9 | 35.37 ± 0.8 * | 11.48 ± 0.5 ** | 12.72 ± 0.7 * |
48 | 21.72 ± 0.4 ** | 2.57 ± 0.1 * | 5.01 ± 0.6 * | 40.38 ± 1.5 ** | 10.45 ± 0.3 * | 10.68 ± 0.2 ** | |
72 | 21.00 ± 1.1 * | 2.79 ± 0.2 ** | 4.46 ± 0.3 | 41.00 ± 1.5 ** | 10.56 ± 0.2 | 9.05 ± 0.6 * | |
96 | 22.95 ± 0.7 ** | 3.59 ± 0.1 * | 4.18 ± 0.2 * | 41.97 ± 1.3 * | 11.36 ± 0.3 | 9.88 ± 0.6 * | |
Mc-cmalB-5 | 24 | 18.95 ± 0.7 * | 2.14 ± 0.3 * | 4.88 ± 0.4 | 39.20 ± 0.7 * | 12.92 ± 0.2 * | 13.49 ± 0.4 * |
48 | 21.82 ± 0.6 ** | 2.99 ± 0.2 * | 6.95 ± 0.4 * | 43.74 ± 1.3 * | 11.89 ± 0.3 ** | 10.89 ± 0.3 ** | |
72 | 22.75 ± 0.9 ** | 3.23 ± 0.4 | 5.31 ± 0.3 | 45.06 ± 1.5 ** | 11.45 ± 0.4 ** | 10.30 ± 0.2 * | |
96 | 23.66 ± 0.2 *** | 4.89 ± 0.3 * | 4.94 ± 0.2 * | 45.10 ± 1.8 * | 12.26 ± 0.1 | 10.35 ± 0.4 * |
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Fazili, A.B.A.; Shah, A.M.; Naz, T.; Nosheen, S.; Yang, W.; Garre, V.; Majeed, Y.; Al-Sadoon, M.K.; Song, Y. Role of Cytosolic Malic Enzyme in Oleaginicity of High-Lipid-Producing Fungal Strain Mucor circinelloides WJ11. J. Fungi 2022, 8, 265. https://0-doi-org.brum.beds.ac.uk/10.3390/jof8030265
Fazili ABA, Shah AM, Naz T, Nosheen S, Yang W, Garre V, Majeed Y, Al-Sadoon MK, Song Y. Role of Cytosolic Malic Enzyme in Oleaginicity of High-Lipid-Producing Fungal Strain Mucor circinelloides WJ11. Journal of Fungi. 2022; 8(3):265. https://0-doi-org.brum.beds.ac.uk/10.3390/jof8030265
Chicago/Turabian StyleFazili, Abu Bakr Ahmad, Aabid Manzoor Shah, Tahira Naz, Shaista Nosheen, Wu Yang, Victoriano Garre, Younis Majeed, Mohammed Khalid Al-Sadoon, and Yuanda Song. 2022. "Role of Cytosolic Malic Enzyme in Oleaginicity of High-Lipid-Producing Fungal Strain Mucor circinelloides WJ11" Journal of Fungi 8, no. 3: 265. https://0-doi-org.brum.beds.ac.uk/10.3390/jof8030265