Effect of Recombinant Antibodies and MIP Nanoparticles on the Electrical Behavior of Impedimetric Biorecognition Surfaces for SARS-CoV-2 Spike Glycoprotein: A Short Report
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents and Solutions
2.2. Antigens and Recombinant Antibodies
2.3. Gold Electrodes Pretreatment in Acid Solution
2.4. Gold Substrate Modification
2.5. Impedimetric BioS Exposure to SARS-CoV-2 Spike Glycoprotein
2.6. Electrochemical Assays
3. Results
3.1. Electrochemical Features of the Modified Surfaces
3.2. Comparison of Immunosensor Response
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Surface Modification Steps | |||||
Rct (Ω) | Cdl (nF) | Φ | Χ2 (Cdl fit) | Χ2 (CPE fit) | |
AuSPE | 222.3 | 5333 | 0.906 | 0.0027 | 0.0007 |
MUDA | 616.4 | 5231 | 0.936 | 0.0031 | 0.0007 |
EDC/NHS | 724.6 | 5209 | 0.931 | 0.0040 | 0.0009 |
Immunosensor Response Post BSA-Blocking | |||||
Rct (Ω) | Cdl (nF) | Φ | Χ2 (Cdl fit) | Χ2 (CPE fit) | |
MIP | 1687 | 2292 | 0.950 | 0.0028 | 0.0005 |
NIP | 4458 | 1865 | 0.933 | 0.0087 | 0.0002 |
R107 | 1809 | 2362 | 0.970 | 0.0017 | 0.0007 |
RBD 1106 | 2161 | 2579 | 0.948 | 0.0022 | 0.0003 |
RBD 5305 | 2286 | 2507 | 0.930 | 0.0032 | 0.0004 |
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Thomaz, D.V.; Goldoni, R.; Tartaglia, G.M.; Malitesta, C.; Mazzotta, E. Effect of Recombinant Antibodies and MIP Nanoparticles on the Electrical Behavior of Impedimetric Biorecognition Surfaces for SARS-CoV-2 Spike Glycoprotein: A Short Report. Electrochem 2022, 3, 538-548. https://0-doi-org.brum.beds.ac.uk/10.3390/electrochem3030037
Thomaz DV, Goldoni R, Tartaglia GM, Malitesta C, Mazzotta E. Effect of Recombinant Antibodies and MIP Nanoparticles on the Electrical Behavior of Impedimetric Biorecognition Surfaces for SARS-CoV-2 Spike Glycoprotein: A Short Report. Electrochem. 2022; 3(3):538-548. https://0-doi-org.brum.beds.ac.uk/10.3390/electrochem3030037
Chicago/Turabian StyleThomaz, Douglas Vieira, Riccardo Goldoni, Gianluca Martino Tartaglia, Cosimino Malitesta, and Elisabetta Mazzotta. 2022. "Effect of Recombinant Antibodies and MIP Nanoparticles on the Electrical Behavior of Impedimetric Biorecognition Surfaces for SARS-CoV-2 Spike Glycoprotein: A Short Report" Electrochem 3, no. 3: 538-548. https://0-doi-org.brum.beds.ac.uk/10.3390/electrochem3030037