Ethanol Extract of Amomum tsao-ko Ameliorates Ovariectomy-Induced Trabecular Loss and Fat Accumulation
Abstract
:1. Introduction
2. Results and Discussion
2.1. EEAT Inhibits RANKL Signaling during Osteoclast Differentiation
2.2. EEAT Ameliorates Bone Loss in OVX Mice
2.3. EEAT Inhibits Fat Accumulation in OVX Mice
2.4. Phytochemical Profiling of EEAT
3. Materials and Methods
3.1. Materials
3.2. Osteoclast Culture and Bone Resorption Assay
3.3. Quantitative Real-Time Polymerase Chain Reaction (RT-PCR)
3.4. Western Blot
3.5. Animal Study
3.6. Micro-CT Analysis
3.7. Measurement of Bone Turnover Markers
3.8. Histological Analysis
3.9. UHPLC–MS/MS
3.10. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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No | Rt (Min) | Calculated (m/z) | Estimated (m/z) | Adducts | Error (ppm) | Formula | MS/MS Fragments (m/z) | Identifications |
---|---|---|---|---|---|---|---|---|
1 | 5.06 | 139.0390 | 139.0388 | [M + H]+ | −1.5296 | C7H6O3 | - | p-Hydroxybenzoic acid * |
2 | 6.52 | 153.0546 | 153.0543 | [M + H]+ | −1.852 | C8H8O3 | 153.0542, 125.0595, 93.0338 | Vanillin or isovanillin * |
3 | 5.64 | 289.0718 | 289.0723 | [M − H]− | 1.8547 | C15H14O6 | 289.0721, 245.0818, 203.0710, 179.0342 | Epicatechin * |
4 | 6.53 | 609.1461 | 609.1472 | [M − H]− | 1.817 | C27H30O16 | 609.1463, 301.0349, 300.0278 | Rutin * |
5 | 6.79 | 463.0882 | 463.0890 | [M − H]− | 1.7702 | C21H20O12 | 463.0888, 301.0348, 300.0278 | Isoquercitrin * |
6 | 7.12 | 303.0510 | 303.0515 | [M − H]− | 1.596 | C15H12O7 | 285.0408, 177.0185, 125.0231 | Taxifolin * |
7 | 9.48 | 315.1602 | 315.1606 | [M − H]− | 1.554 | C19H24O4 | 315.1605, 149.0597 | Meso-hannokinol or (+)-hannokinol |
8 | 9.58 | 301.0354 | 301.0358 | [M − H]− | 1.3856 | C15H10O7 | 301.0356, 178.9977, 151.0026 | Quercetin * |
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Shim, K.-S.; Hwang, Y.-H.; Jang, S.-A.; Kim, T.; Ha, H. Ethanol Extract of Amomum tsao-ko Ameliorates Ovariectomy-Induced Trabecular Loss and Fat Accumulation. Molecules 2021, 26, 784. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules26040784
Shim K-S, Hwang Y-H, Jang S-A, Kim T, Ha H. Ethanol Extract of Amomum tsao-ko Ameliorates Ovariectomy-Induced Trabecular Loss and Fat Accumulation. Molecules. 2021; 26(4):784. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules26040784
Chicago/Turabian StyleShim, Ki-Shuk, Youn-Hwan Hwang, Seon-A Jang, Taesoo Kim, and Hyunil Ha. 2021. "Ethanol Extract of Amomum tsao-ko Ameliorates Ovariectomy-Induced Trabecular Loss and Fat Accumulation" Molecules 26, no. 4: 784. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules26040784