Next Article in Journal
Polyphenolic Characterization, Antioxidant, Antihyaluronidase and Antimicrobial Activity of Young Leaves and Stem Extracts from Rubus caesius L.
Next Article in Special Issue
Detection and Verification of a Key Intermediate in an Enantioselective Peptide Catalyzed Acylation Reaction
Previous Article in Journal
Comparison of Different Volatile Extraction Methods for the Identification of Fishy Off-Odor in Fish By-Products
Previous Article in Special Issue
Extraction of the Anticancer and Antimicrobial Agent, Prodigiosin, from Vibrio gazogenes PB1 and Its Identification by 1D and 2D NMR
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 27
Issue 19
10.3390/molecules27196179
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Identification of Photocatalytic Alkaloids from Coptidis Rhizome by an Offline HPLC/CC/SCD Approach

by
Shu-Qin Qin
1,†,
Jun Ma
2,†,
Qi-Qi Wang
1,
Wei Xu
1,
Wen-Cai Ye
1,* and
Ren-Wang Jiang
1,*
1
Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, China
2
Research Institute of Guangdong HAID Group Co., Ltd., Guangzhou 511400, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Molecules 2022, 27(19), 6179; https://0-doi-org.brum.beds.ac.uk/10.3390/molecules27196179
Submission received: 6 September 2022 / Revised: 16 September 2022 / Accepted: 17 September 2022 / Published: 21 September 2022
(This article belongs to the Special Issue Spectroscopic and Spectrometric Techniques for Structural Analysis)
Browse Figures
Review Reports Versions Notes

Abstract

:
Natural products continue to be a valuable source of active metabolites; however, researchers of natural products are mostly focused on the biological effects, and their chemical utility has been less explored. Furthermore, low throughput is a bottleneck for classical natural product research. In this work, a new offline HPLC/CC/SCD (high performance liquid chromatography followed by co-crystallization and single crystal diffraction) workflow was developed that greatly expedites the discovery of active compounds from crude natural product extracts. The photoactive total alkaloids of the herbal medicine Coptidis rhizome were firstly separated by HPLC, and the individual peaks were collected. A suitable coformer was screened by adding it to the individual peak solution and observing the precipitation, which was then redissolved and used for co-crystallization. Seven new co-crystals were obtained, and all the single crystals were subjected to X-ray diffraction analysis. The molecular structures of seven alkaloids from milligrams of crude extract were resolved within three days. NDS greatly decreases the required crystallization amounts of alkaloids to the nanoscale and enables rapid stoichiometric inclusion of all the major alkaloids with full occupancy, typically without disorder, affording well-refined structures. It is noteworthy that anomalous scattering by the coformer sulfur atoms enables reliable assignment of absolute configuration of stereogenic centers. Moreover, the identified alkaloids were firstly found to be photocatalysts for the green synthesis of benzimidazoles. This study demonstrates a new and green phytochemical workflow that can greatly accelerate natural product discovery from complex samples.

1. Introduction

Natural products are produced naturally by any organism including primary and secondary metabolites. In a broad sense, they include any substance produced by life. Worldwide revitalization has been taking place in the interest for natural product research [1]. In daily life, bombardment with advertisements for natural and organic products is very frequent. However, current natural product research still faces challenges, e.g., traditional research strategies and low efficient protocols. On one hand, researchers of natural products are mostly focused on the biological effects. Owing to the chemical diversity, target affinity, and specificity toward biological macromolecules, natural products derived from herbal medicines have been extensively applied in biological assays and have demonstrated enormous potential as modulators of biomolecular functions. The resulting lead compounds often play a significant role in drug discovery [2]. On the other hand, classical natural product research has low efficiency. Starting with a bulk quantity of raw material, through extraction and isolation, often results in micrograms of natural products [3]. Then, extensive spectroscopic analyses were used to elucidate the structures. The whole process consumes a large quantity of raw material and organic solvent and need months or years of time [4]. The high cost and low efficiency constitute major bottlenecks in the field of natural product chemistry, and development of new protocol is necessary. We reported herein the identification of photocatalytic alkaloids from Coptidis rhizome (CR) by an offline HPLC/CC/SCD (high performance liquid chromatography followed by co-crystallization and single crystal diffraction) approach and its applications as green photocatalysts for the synthesis of benzimidazoles.
The procedure for the offline HPLC/CC/SCD workflow is described in Figure 1. The first step was to produce a chromatogram from a crude natural sample by preparative HPLC, a separation technique that can selectively produce single peaks of natural compounds from crude extracts on a nanogram scale in a short time. In the second step, the individual peaks in the HPLC profile were collected and concentrated in vacuo. In the third step, the residue for the individual peaks were added a co-crystallization coformer 1,5-naphthalenedisulfonic acid (NDS) and subjected to crystal growth. Finally, the resulting co-crystals were analyzed by X-ray crystallographic analysis. After collection of diffraction data, the structures of the alkaloid guests were elucidated by X-ray crystallographic analysis.

2. Results

2.1. Photocatalysis of Benzimidazole Catalysed by the Raw Material and Total Alkaloids under Blue LED Irradiation

Benzimidazole and its derivatives are widely employed as privileged lead structures in drug discovery because of the unique structural features and a wide range of biological activities, e.g., anthelmintic [5], anti-tuberculosis [6], anti-hypertension [7], and antiproliferation [8]. O-phenylenediamine and benzaldehydes were used as substrates and dried CR powder was used as photocatalysts for the synthesis of benzimidazole under blue LED (470 nm) irradiation (Scheme 1, and for the 1H-, 13C-NMR, and MS, please see supporting information: Figure Sa–c). A yield of 43.4% was achieved when the powder of CR was added as the catalyst (3% of the mass of o-phenylenediamine) (Table 1). In contrast, only a trace amount of product was formed when there was no light source, short irradiation time (2 h), or no catalyst, indicating that the powder of CR possessed photocatalytic activity. Isoquinoline alkaloids are the main active ingredients of CR. Thus, the total alkaloid was extracted (yield 8%) from CR powder (1 g). The catalytic efficiency of total alkaloids was 58.2% (Table 1), indicating that the alkaloid is the major active ingredient responsible for the catalyzing capacity of CR.

2.2. HPLC Analysis of the Total Alkaloid and Prescreening of Coformers

In order to characterize the catalytically active components in the total alkaloid, we performed HPLC analysis. The chromatogram of the total alkaloid showed seven major peaks (Figure 2a). The methanol solution of the total alkaloid was tested against a panel of coformers possessing an acid group or π-electron system, i.e., carboxylic acids (fumaric acid (i) [9] and succinic acid (ii) [10]), organic base (theophylline (iii) [11]), and sulfonic acids (1,5-naphthalenedisulfonic acid (NDS, iv) and 2-naphthalenesulfonic acid (v)). After 0.5 h, only tube (iv) that had NDS added showed precipitate (Figure 3). After 2.0 h, more precipitate was formed in tube (iv), while only a small amount of precipitate appeared in tube (i). However, other tubes still remained clear after 2.0 h (Figure 3). The resulting precipitate in tube (iv) was centrifuged and dissolved in methanol. After filtration through a 0.22 μm membrane, it was analyzed by HPLC in the same condition as the total alkaloid (Figure 2b). We found that the precipitate formed by NDS contained all the seven peaks as the original total alkaloids, except for the relatively increased intensity of peaks 2–7 (Figure 2c), indicating that NDS has strong interactions with all these peaks. Although the relative intensity for peak 1 remained largely unchanged as compared to peaks 2–7, this component still exhibited sufficient interaction with NDS (Figure 2c).
Then, all the seven peaks in the total alkaloid chromatogram generated by semi-preparative HPLC were collected individually and concentrated in a SpeedVac to afford compounds 1–7. A certain amount of NDS (based on the stoichiometric ratio from 1H-NMR analysis of the precipitate) in methanol solution was added to the residue of each compound. The mixed solutions were kept at room temperature to grow single crystals. It is noteworthy that coformers can be efficiently pre-screened for their binding affinity to the analysis by a comparison of the high performance liquid chromatography (HPLC) chromatograms of the crude extract before and after the addition of coformers.

2.3. Identification of the Individual Component by Co-Crystallization

NDS was found to be a suitable coformer in our pre-screening test. The precipitate formed by adding NDS to the individual peak solution (collected through semi-preparative HPLC. Peaks 3 and 4 are two close peaks. Collection of these two peaks should be undertaken very carefully, and only narrow peak time could be collected.) was filtered and dissolved in methanol, and new co-crystals of NDS with the seven peaks were obtained at room temperature in stoichiometric quantities through a single-step crystallization.
1H-NMR spectrum of the precipitate formed by adding NDS to compound 1 showed 1:2 stoichiometric ratio. The up-field shifted resonance of Ha (−0.05 ppm), Hb (−0.07 ppm) and Hc (−0.01 ppm) on the benzene ring of NDS (Table 2) indicated the formation of inclusion complex (Figure 4a), which was also supported by NOESY spectrum, i.e., NOE correlations were observed between NDS and 1 (supporting information, Figure S1a). Furthermore, inclusion complex of (NDS) ⊃12 (the ⊃ symbol denotes inclusion) formed well-defined single crystals by slow evaporation of the methanol solution. Single-crystal X-ray diffraction data revealed that (NDS) ⊃12 crystallized in the triclinic P1 space group (Table 3). Compound 1 (C20H24NO4+) is a quaternary benzylisoquinoline alkaloid of the aporphine structural subgroup with a tetracyclic ring system bearing two hydroxy groups at the C-1 and C-11, two methoxy groups at the C-2 and C-10 positions, and two methyl groups on the nitrogen atom. From the Flack parameter calculated by the Parsons’ method [x = 0.06(3)], the absolute configuration of compound 1 was also determined (Figure 4b,c). By a literature search, compound 1 was identified as the known natural product α-magnolflorine [12]; however, its crystal structure and absolute configuration were established here for the first time. In a previous study [12], the work of Chen and co-investigators confirmed the presence of two isoforms of magnolflorine that exhibit different pharmacological activities. Our co-crystal X-ray analysis directly secured both the relative and absolute structure of compound 1.
The asymmetric unit consist of one NDS, one methanol, two water and two molecules of 1 (Figure 4d), which showed the same structures with slightly different orientation of the methoxy group at C-10. Though there were three solvent molecules, none of them were disordered. The crystalline 1:2 ratio confirmed the result from NMR analysis. NDS and 1 was connected by various intermolecular C-H···O interactions (supporting information, Figure S1b) and C-H···π interactions (supporting information, Figure S1c) into a three-dimensional network (Figure 4e).
Similarly, the 1H-NMR spectrum of the precipitate formed by adding NDS to compounds 2 or 3 (Figure 5(2a,3a)) showed a 1:2 stoichiometric ratio. The Ha, Hb, and Hc of NDS demonstrated significant up-field shifts (−0.06, −0.08, and −0.02 ppm, respectively, when added to 2 or 3 (Table 2)) (supporting information, Figures S2a and S3a), indicating strong intermolecular interactions. Formation of inclusion complexes was also supported by 2D NOESY spectrum, i.e., NOE correlations were observed between NDS and 2 or 3 (supporting information, Figures S2b and S3b). Compounds 2 and 3 formed well-defined co-crystals with NDS. Single crystal structure analysis revealed that (NDS) ⊃ 22 and (NDS)1/2 ⊃ 3 crystallized in the monoclinic space groups Cc and P21/n, respectively (Table 3). Figure 5(2b) and Figure 6a showing the X-ray structure and packing diagram, respectively, for 2 and (NDS) ⊃ 22; and Figure 5(3b) and Figure 6b showing the X-ray structure and packing diagram, respectively, for 3 and (NDS)1/2 ⊃ 3). Compound 2 bears a quaternary isoquinoline alkaloid skeleton with a 9-hydroxy-1, 10, 17-trimethoxy substitution pattern (C20H20NO4+). Compound 3 is similar to 2 (C20H20NO4+), with a different substitution pattern (10-hydroxy-1,9,17-trimethoxy). The asymmetric unit of (NDS) ⊃ 22 is composed of an NDS molecule, two compound 2 molecules, and two methanol molecules (without disorder) (Figure 5(2c)). In contrast, the asymmetric unit of (NDS)1/2 ⊃ 3 is composed of half an NDS molecule (located at the inversion center) and one molecule of 3 (same stoichiometric ratio 1:2 as 2) but no solvent molecule (Figure 5(3c)). By a literature search, compound 2 was identified as the known natural product columbamine, which was reported to suppress hepatocellular carcinoma cells through down regulation of PI3K/AKT, p38, and ERK1/2 MAPK signaling pathways [13], while compound 3 was identified as the known natural product jatrorrhizine, which was reported to be a potent antagonist against the transmembrane protease to impact COVID-19 drug therapy [14].
Compounds 4 and 5 (Figure 5(4a,5a)) were two minor peaks. Especially, 4 is the shoulder of 3 in the chromatogram (Figure 2b). These two compounds were easily skipped in classic natural product chemistry. Precipitates occurred when NDS in methanol was added to 4 or 5. 1H-NMR analysis on the precipitates revealed up-field chemical shifts for Ha, Hb, and Hc (−0.07, −0.08, and −0.02 ppm when NDS was added to 4, and −0.06, −0.08, and −0.02 ppm when NDS was added to 5) (Table 2), indicating the strong interactions between NDS and 4 or 5 (supporting information, Figures S4a and S5a), which were also confirmed by the NOESY spectra of these two inclusion complexes (supporting information, Figures S4b and S5b). Then, the precipitates were filtered, and the filtrates were subjected to crystal growth. Crystals appeared within two days. Single crystal X-ray analysis revealed that both (NDS)1/2 ⊃ 4 and (NDS) ⊃ 52 crystallized in the same triclinic space group P-1 (Table 3). Figure 5(4b) and Figure 6c showing the X-ray structure and packing diagram, respectively, for 4 and (NDS)1/2 ⊃ 4; and Figure 5(5b) and Figure 6d showing the X-ray structure and packing diagram, respectively, for 5 and (NDS) ⊃ 52). The asymmetric unit of (NDS)1/2 ⊃ 4 is composed of half an NDS molecule at the inversion center and one molecule of 4 (stoichiometric ratio 1:2) (Figure 5(4c)). In contrast, the asymmetric unit of (NDS) ⊃ 52 consists of an NDS molecule and two molecules of 5 (same stoichiometric ratio 1:2 as 4) (Figure 5(5c)). Compound 4 (C20H18NO4) bears a pentacyclic protoberberine skeleton with two methoxy groups at C-9 and C-10, and a methylenedioxy group at C-1 and C-17. Accordingly, compound 4 was identified as the known natural product epiberberine, which was reported to be a potent inhibitor against the ureases from Helicobacter pylori [15]. The skeleton of 5 is the same as that of 4, but the two methoxy groups at C-9 and C-10 in 4 were replaced by a methylenedioxy group in 5. Accordingly, compound 5 (C19H14NO4) was identified as the known natural product coptisine, which was found to inhibit plasmodium falciparum dihydroorotate dehydrogenase, a promising drug target for antimalarial chemotherapy [16]. This compound is also a promising candidate agent for prevention of diabetic neuropathy [17].
Compounds 6 and 7 (Figure 5(6a,7a)) were the last two peaks in the precipitate chromatogram (Figure 2b). The 1H-NMR spectrum on the precipitate formed by adding NDS to 6 or 7 showed up-field chemical shifts for Ha, Hb, and Hc (−0.07, −0.10, and −0.03 ppm for 6, and −0.06, −0.09, and −0.02 ppm for 7 (Table 2)) (supporting information, Figures S6a and S7a), indicating significant interactions, which were again confirmed by 2D-NOESY spectra of these two inclusion complexes (supporting information, Figures S6b and S7b). Both inclusion complexes of (NDS)1/2 ⊃ 6 and (NDS)1/2 ⊃ 7 formed well-defined single crystals by slow evaporation of the methanol solution at room temperature. Single-crystal X-ray diffraction data revealed that (NDS)1/2 ⊃ 6 and (NDS)1/2 ⊃ 7 crystallized in the same triclinic P-1 space group (Table 3). Figure 5(6b) and Figure 6e showing the X-ray structure and packing diagram, respectively, for 6 and (NDS)1/2 ⊃ 6; and Figure 5(7b) and Figure 6f showing the X-ray structure and packing diagram, respectively, for 7 and (NDS)1/2 ⊃ 7). Compound 6 (C21H22NO4+) is a quaternary protoberberine alkaloid with four methoxy groups at C-1, C-9, C-10, and C-17 positions. Compound 7 (C21H22NO4+) has the same skeleton as 6, but the methoxy groups at C-9 and C-10 were changed to a methylenedioxy group. By a literature search, compounds 6 and 7 were identified as the known natural product palmatine and berberine (C20H18NO4+), respectively. Both compounds were active on the α2-adrenergic receptor with IC50 at the nanomole levels, and inhibit the multiplication of bacteria, fungi, and viruses [18]. Both the asymmetric units of (NDS)1/2 ⊃ 6 and (NDS)1/2 ⊃ 7 contain half an NDS molecule located at the inversion center and one molecule of compound 6 or 7 (Figure 5(6c,7c)). Due to the strong C-H···O and C-H···π interactions, no disorder was observed in these crystal structures (supporting information, Figures S6c,d and S7c,d).

2.4. NDS Decreases the Required Amounts of Alkaloids for Crystallization to Nanoscale

NDS greatly decreases the required crystallization amounts of alkaloids. Compound 3 (jatrorrhizine) was used as an example. When 3 (0.5 μg/μL, 0.7 μL) was layered with NDS (0.5 μg/μL, 0.7 μL) in a capillary tube (diameter 0.3 mm), co-crystals formed within 1 h. In contrast, no crystal was observed when keeping 3 alone. Thus, the amount required for the crystallization of 3 was only 0.5 μg/μL × 0.7 μL = 350 ng (Figure S8). It is noteworthy that compound 4 is the shoulder of 3 in the chromatogram (Figure 2b). Though we could only purify small amounts of 4 and 3 (baseline separation of 3 and 4 was not achieved), with the help of NDS, only a nanoscale of compounds was needed to be obtained for pure enough co-crystals for the SCD analysis.

2.5. Photocatalysis by the Individual Alkaloids

Through offline HPLC-CC-SCD analysis, compounds 1–7 in the active total alkaloid were identified as magnoflorine (Figure 4b), columbamine (Figure 5(2a)), jatrorrhizine (Figure 5(3a)), epiberberine (Figure 5(4a)), coptisine (Figure 5(5a)), palmatine (Figure 5(6a)), and berberine (Figure 5(7a)), respectively. To explore the active component responsible for the catalytic efficiency of the herbal powder and total alkaloid, we compared the yield of individual alkaloids of CR in the photocatalytic reaction. Surprisingly, all alkaloids had catalytic ability with a yield in the range 54.6–82.1%. Compound 1 (magnoflorine), bearing a short conjugation system, showed the lowest catalytic efficiency (54.6%), which is higher than the powder of CR but slightly lower than total alkaloid (58.2%). Compounds 2–7, possessing the isoquinoline alkaloid skeleton with a long conjugation system, showed excellent catalytic efficiency (71.2–82.1%) (Table 1).

3. Discussion

Traditionally, the synthesis of benzothiazoles involved the condensation of phenylenediamine and its derivatives with carboxylic acids or aldehydes [19]. In recent years, catalyzed synthesis using different Lewis acids as the catalysis, e.g., SnCl2, appeared to improve the yield [20]. Visible light as a convenient and renewable clean energy source has attracted increasing interest in organic photosynthesis [21]. Compared with traditional synthesis and heavy metal catalysis, visible light catalysis meets the requirements of green chemistry and environmental friendliness. Very recently, synthesis of benzothiazoles using fluorescein as an efficient photocatalyst under visible light was reported in high to excellent yields [22]. However, photocatalysts using herbal materials were less reported.
CR is derived from the roots of Coptis chinensis, a species of flowering plant, which is traditionally used as an antibacterial herb [23]. This herb is a rich source of isoquinoline alkaloids with conjugated chromophores, e.g., berberine and palmatine. Besides the bactericidal activity, it is noteworthy that this class of alkaloids were found to show strong photodynamic effects. Berberine is an efficient photosensitizer. Its association with photodynamic therapy may be a potential anticancer treatment strategy for cervical cancer [24]. The DNA damage generated by a combination of berberine with UVA irradiation induced a significant blockage of EAC cells (Ehrlich ascites carcinoma) in the S and G2/M phases and the decrease of cell proliferation after 24 h of treatment [25]. Photodynamic therapy of palmatine exhibited a potent phototoxic effect in cell proliferation and produced cell apoptosis [26].
Considering the strong photodynamic effect of isoquinoline alkaloids and the conjugated skeleton, in this context, we demonstrated that the raw material Coptidis rhizome and the major isoquinoline alkaloids could be used as visible light catalysis for the green synthesis of benzimidazole. Furthermore, a HPLC based pre-screening approach was established for the selection of a suitable coformer. The subsequent single crystal X-ray diffraction analysis enables the rapid structural analysis of the photoactive molecules in the context of a hydrogen-bonded network without the necessity for massive phytochemical isolation.
Notably, these alkaloids possess a relatively low concentration level in plants, which makes their exact structure hard to analyze via spectral analysis in a short time. The most accurate method to determine the three-dimensional structure of molecules is single-crystal X-ray crystallography. However, this method requires the molecules to be crystallized and single crystals should be obtained before analysis. Several approaches are being pursued to address this limitation: (i) Host–guest chemistry. Cucurbituril [27], porphyrins [28], crown ether [29], calixarenes [30], cyclodextrins [31], co-crystals [32,33], MOF [34], and the recently reported mechanically interlocked structures [35] have proven abilities to capture a broad range of different organic molecules. (ii) Hydrogen-bonded frameworks. Guanidinium organosulfonates were found to capture various guest molecules of interest through the formation of hydrogen-bonded frameworks [36]. (iii) Crystal sponge. This method uses a metal–organic framework to absorb guest compounds, which were efficiently trapped and concentrated in the periodic array of the binding sites of the porous complexes either through intermolecular interactions [37] or coordinative alignments [38]. Furthermore, recently, scientists found that an ultrastable π–π stacked porous organic molecular framework can also be used as a crystalline sponge [39]. (iv) DNA duplex sequence. This method used an oligonucleotide sequence to capture the guest molecules as minor groove binder [40].
In this context, we successfully discovered a coformer to help arrange these low concentration molecules into a highly ordered state in the co-crystals. Through X-ray crystallography, these alkaloid guests’ detailed structures were clearly and rapidly elucidated, and the specific interactions between the co-crystal were proved. 1,5-Naphthalenedisulfonic acid (NDS) as a new family member of coformer can be regarded as an ideal candidate for the structural determination of certain alkaloids. It is noteworthy that the calculated electrostatic potential surface of NDS showed a strong negative charge area around the 1,5-disulfonate groups, which were consistent with the strong interactions with the alkaloids in CR with positive nitrogen atoms (Figure 7).

4. Materials and Methods

4.1. Extraction of Total Alkaloids from CR

Commercially purchased CR herb was crushed and sieved to obtain CR powder. The powder (1 g) was extracted by ultrasonic treatment with 95% EtOH at room temperature three times. The solution was filtered and concentrated under reduced pressure to obtain the crude extract. Then, an appropriate amount of 2% HCl solution was added to solve the crude extract and adjust the pH = 4. The solution was filtered, and the filtrate was alkalized with NaOH to pH = 9, which was then extracted with dichloromethane. Finally, the solvent was removed under reduced pressure to afford the total alkaloids of CR (80 mg).

4.2. Separation of Individual Alkaloids

The total alkaloids were subjected to semi-preparative HPLC on a Wufeng LC-100 system (Shanghai, China) with column: ultimate XB-C18 column (250 × 10.0 mm, 5 μm); mobile phase: 0.1% formic acid water (A) acetonitrile (B); gradient: 5 → 15% B in 0–10 min; 15 → 25% B in 10–15 min; 25% B in 15–35 min; flow rate: 3.0 mL/min, detection: UV at 278 nm. The collected solution of each peak was concentrated in a SpeedVac to afford compounds 1–7.

4.3. Co-Crystallization

NDS was added to the solution of individual peaks of the total alkaloids. The precipitate formed by adding NDS was filtered and dissolved in methanol, and co-crystals of NDS with the seven peaks were obtained at room temperature.

4.4. Single-Crystal X-ray Diffraction Analysis

All single-crystal measurements were performed on a Rigaku Oxford diffractometer (Rigaku, Co. Tokyo, Japan) using CuKα (λ = 1.54056 Å) radiation. The structures were solved by direct methods (SHELXTL-2014) and refined by full-matrix least-squares on F2. Crystallographic data in standard CIF format were deposited with the Cambridge Crystallographic Data Centre. The CCDC numbers are shown in Table 3. Copies of the data can be obtained, free of charge, on application to CCDC, 12 Union Road, Cambridge CB2 1EZ, U.K. (fax: (+44)1223-336033; e-mail: [email protected]).

4.5. Electrostatic Potential Surfaces

All structures (generated by Chemdraw) were fully optimized at the B3LYP/6-31G(d) theoretical level. Vibrational analyses at the same level of theory were performed to confirm each stationary point to a local minimum. Electrostatic potential surface (ESP) values were calculated at the B3LYP/6-31G(d) level in the Gaussian 09 program. The electrostatic potential surfaces (ESP) of computed species were generated with Gaussview 6.0.

4.6. Synthesis of Benzimidazole Using CR and the Alkaloids as Catalysts

Benzaldehyde (1a, 0.1 mmol), o-phenylenediamine (1b, 0.1 mmol), acetonitrile (1.5 mL), and catalysts (including the herbal power, total alkaloid, and individual alkaloid, 3%) were added in a tube (10 mL). The open-air reaction tube was placed under a 5 W blue LED lamp and stirred at room temperature for 12 h. The reaction was extracted with ethyl acetate, and the organic layer was washed with water. The organic layer was saturated with sodium chloride and separated. Then the organic layer was dried with anhydrous Na2SO4 and concentrated under reduced pressure. The crude product was purified through silica gel column chromatography (petroleum ether: ethyl acetate = 2:1) to afford a light-yellow product (1c). The yield was calculated by the HPLC peak area using the purified 1c as the standard. 1H NMR (400 MHz, DMSO) δ 12.93 (s, 1H), 8.23–8.15 (m, 2H), 7.65–7.44 (m, 5H), 7.25–7.15 (m, 2H). 13C NMR (100 MHz, DMSO) δ 151.2, 130.1, 129.8, 128.9, 126.4, 122.1, 115.1.

5. Conclusions

In summary, our pre-screening with HPLC analysis successfully demonstrated that NDS can act as an excellent coformer for the rapid co-crystallization with the major alkaloids in CR. The structures of the seven new co-crystals were then determined by X-ray crystallographic analysis. This work provides a new efficient workflow featuring the offline HPLC/CC/SCD strategy and no massive phytochemical work was conducted. NDS greatly decreases the required crystallization amounts of alkaloids to the nanoscale. Furthermore, all the identified alkaloids were firstly found to show photocatalytic ability with the yields in the range 54.6–82.1%. The photocatalytic ability was found to be related to the conjugation system. Compound 1 (magnoflorine), bearing a short conjugation system, showed the lowest catalytic efficiency, while Compounds 2–7, possessing a long conjugation system, showed excellent catalytic efficiency. Accordingly, besides the new HPLC/CC/SCD workflow, this work sheds light on the photocatalytic potential of natural products, though the biological effects of natural products were widely reported.

Supplementary Materials

The following supporting information can be downloaded at: https://0-www-mdpi-com.brum.beds.ac.uk/article/10.3390/molecules27196179/s1, Figure Sa: 1H-NMR spectra of compound 1c (400 MHz, DMSO); Figure Sb: 13C-NMR spectra of compound 1c (100 MHz, DMSO); Figure Sc: ESI-MS spectra of compound 1c ([M + H]+, observed m/z: 195.0911, calcd. m/z: 195.0916); Figures S1a–S7d: NMR and crystal structures of NDS ⊃ (1–7); Figure S8: Nanoscale crystallization experiment of NDS and 3 (jatrorrhizine).

Author Contributions

S.-Q.Q. performed the co-crystallization and photocatalytic synthesis of benzimidazoles. J.M. and Q.-Q.W. carried out the HPLC analysis before and after adding coformer. W.X. performed the major parts of the X-ray studies. W.-C.Y. and R.-W.J. designed the experiment. S.-Q.Q. and J.M. contributed equally to this work. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the National Natural Science Foundation of China (81872760) and Natural Science Foundation of Guangdong province (2021A11515011251) and Guangdong scientific scheme (2021A0505030032).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The data presented in this study are available in the supplementary material.

Acknowledgments

Special thanks is given to the public research platform of the College of Pharmacy, Jinan University and the X-ray diffraction facility in South China Sea Institute of Oceanology, Chinese Academy of Sciences.

Conflicts of Interest

The authors declare no conflict of interest.

Sample Availability

Samples of the NDS ⊃ 12 and NDS ⊃ 22 and (NDS)1/2 ⊃ 3 and (NDS)1/2 ⊃ 4 and NDS ⊃ 52 and (NDS)1/2 ⊃ 6 and (NDS)1/2 ⊃ 7 are available from the authors.

References

  1. Paterson, I.; Anderson, E.A. The Renaissance of Natural Products as Drug Candidates. Science 2005, 310, 451–453. [Google Scholar] [CrossRef] [PubMed]
  2. Harvey, A.; Edrada-Ebel, R.; Quinn, R. The re-emergence of natural products for drug discovery in the genomics era. Nat. Rev. Drug Discov. 2015, 14, 111–129. [Google Scholar] [CrossRef] [PubMed]
  3. Atanasov, A.G.; Zotchev, S.B.; Dirsch, V.M.; Supuran, C.T. Natural products in drug discovery: Advances and opportunities. Nat. Rev. Drug Discov. 2021, 20, 200–216. [Google Scholar] [CrossRef] [PubMed]
  4. Chhetri, B.K.; Lavoie, S.; Sweeney-Jones, A.M.; Kubanek, J. Recent trends in the structural revision of natural products. Nat. Prod. Rep. 2018, 35, 514–531. [Google Scholar] [CrossRef]
  5. Velík, J.; Baliharová, V.; Fink-Gremmels, J.; Bull, S.; Lamka, J.; Skálová, L. Benzimidazole drugs and modulation of biotransformation enzymes. Res. Vet. Sci. 2014, 76, 95–108. [Google Scholar] [CrossRef]
  6. Kalalbandi, V.K.A.; Seetharamappa, J.; Katrahalli, U.; Bhat, K.G. Synthesis, crystal studies, anti-tuberculosis and cytotoxic studies of 1-[(2E)-3-phenylprop-2-enoyl]-1H-benzimidazole derivatives. Eur. J. Med. Chem. 2014, 79, 194–202. [Google Scholar] [CrossRef]
  7. Borbaa, P.A.A.; Pinotti, M.; Andradeb, G.R.S.; Costa, N.B., Jr.; Juniord, L.R.O.; Fernandesd, D.; Campose, C.E.M.; Stulzera, H.K. The effect of mechanical grinding on the formation, crystalline changes and dissolution behaviour of the inclusion complex of telmisartan and β-cyclodextrins. Carbohydr. Polym. 2015, 133, 373–383. [Google Scholar] [CrossRef]
  8. Tahlan, S.; Kumar, S.; Kakkar, S.; Narasimhan, B. Benzimidazole scafolds as promising antiproliferative agents: A review. BMC Chem. 2019, 13, 66. [Google Scholar] [CrossRef]
  9. Évora, A.O.L.; Bernardes, C.E.S.; Piedade, M.F.M.; Conceição, A.C.L.; Minas da Piedade, M.E. Energetics of Glycine Cocrystal or Salt Formation with Two Regioisomers: Fumaric Acid and Maleic Acid. Cryst. Growth Des. 2019, 19, 5054–5064. [Google Scholar] [CrossRef]
  10. Alhalaweh, A.; George, S.; Boström, D.; Velaga, S.P. 1:1 and 2:1 Urea−Succinic Acid Cocrystals: Structural Diversity, Solution Chemistry, and Thermodynamic Stability. Cryst. Growth Des. 2010, 10, 4847–4855. [Google Scholar] [CrossRef]
  11. Darwish, S.; Zeglinski, J.; Krishna, G.R.; Shaikh, R.; Khraisheh, M.; Walker, G.M.; Croker, D.M.A. New 1:1 Drug-Drug Cocrystal of Theophylline and Aspirin: Discovery, Characterization, and Construction of Ternary Phase Diagrams. Cryst. Growth Des. 2018, 18, 7526–7532. [Google Scholar] [CrossRef]
  12. Okon, E.; Kukula-Koch, W.; Jarzab, A.; Halasa, M.; Stepulak, A.; Wawruszak, A. Advances in Chemistry and Bioactivity of Magnoflorine and Magnoflorine-Containing Extracts. Int. J. Mol. Sci. 2020, 21, 1330. [Google Scholar] [CrossRef] [PubMed]
  13. Lin, Z.; Li, S.; Guo, P.; Wang, L.; Zheng, L.; Yan, Z.; Chen, X.; Cheng, Z.; Yan, H.; Zheng, C.; et al. Columbamine suppresses hepatocellular carcinoma cells through down regulation of PI3K/AKT, p38 and ERK1/2 MAPK signaling pathways. Life Sci. 2019, 218, 197–204. [Google Scholar] [CrossRef] [PubMed]
  14. Pooja, M.; Reddy, G.J.; Hema, K.; Dodoala, S.; Koganti, B. Unravelling high-affinity binding compounds towards transmembrane protease serine 2 enzyme in treating SARS-CoV-2 infection using molecular modelling and docking studies. Eur. J. Pharmacol. 2021, 890, 173688. [Google Scholar]
  15. Tan, L.; Li, C.; Chen, H.; Mo, Z.; Zhou, J.; Liu, Y.; Ma, Z.; Xu, Y.; Yang, X.; Xie, J.; et al. Epiberberine, a natural protoberberine alkaloid, inhibits urease of Helicobacter pylori and jack bean: Susceptibility and mechanism. Eur. J. Pharm. Sci. 2017, 110, 77–86. [Google Scholar] [CrossRef]
  16. Lang, L.; Hu, Q.; Wang, J.; Liu, Z.; Huang, J.; Lu, W.; Huang, Y. Coptisine, a natural alkaloid from Coptidis Rhizoma, inhibits plasmodium falciparum dihydroorotate dehydrogenase. Chem. Biol. Drug Des. 2018, 92, 1324–1332. [Google Scholar] [CrossRef]
  17. Friedemann, T.; Schumacher, U.; Tao, Y.; Leung, K.M.; Schröder, S. Neuroprotective Activity of Coptisine from Coptis chinensis (Franch). Evid.-Based Complement. Altern. Med. 2015, 2015, 827308. [Google Scholar] [CrossRef]
  18. Schmeller, T.; Latz-Brüning, B.; Wink, M. Biochemical activities of berberine, palmatine and sanguinarine mediating chemical defence against microorganisms and herbivores. Phytochemistry 1997, 44, 257–266. [Google Scholar] [CrossRef]
  19. Akhtar, W.; Khan, M.F.; Verma, G.; Shaquiquzzaman, M.; Rizvi, M.A.; Mehdi, S.H.; Akhter, M.; Alam, M.M. Therapeutic evolution of benzimidazole derivatives in the last quinquennial period. Eur. J. Med. Chem. 2017, 126, 705–753. [Google Scholar] [CrossRef]
  20. Duan, L.; Li, Q.; Wu, N.; Xu, D.; Zang, H. Synthesis of 2,5-disubstitued benzimidazole using SnCl2-catalyzed reduction system at room temperature. Chin. Chem. Lett. 2014, 25, 155–158. [Google Scholar] [CrossRef]
  21. Sun, N.; Qi, D.; Jin, Y.; Wang, H.; Wang, C.; Qu, C.; Liu, J.; Jin, Y.; Zhang, W.; Jiang, J. Porous Pyrene Organic Cage with Unusual Absorption Bathochromic-Shift Enables Visible Light Photocatalysis. CCS Chem. 2021, 3, 2917–2925. [Google Scholar] [CrossRef]
  22. Li, Z.; Song, H.; Guo, R.; Zuo, M.; Hou, C.; Sun, S. Visible-light-induced condensation cyclization to synthesize benzimidazoles using fluorescein as a photocatalyst. Green Chem. 2019, 21, 3602. [Google Scholar] [CrossRef]
  23. Zhang, X.; Sun, X.; Wu, J.; Wu, Y.; Wang, Y.; Hu, X.; Wang, X. Berberine Damages the Cell Surface of Methicillin-Resistant Staphylococcus aureus. Front. Microbiol. 2020, 11, 621. [Google Scholar] [CrossRef] [PubMed]
  24. Pmo, A.; Tzl, B.; Act, C.; Pr, B.; Mfc, B. Effect of berberine associated with photodynamic therapy in cell lines. Photodiagn. Photodyn. 2020, 32, 02045. [Google Scholar]
  25. Jantová, S.; Letasiova, S.; Brezova, V.; Cipak, L.; Labaj, J. Photochemical and phototoxic activity of berberine on murine fibroblast NIH-3T3 and Ehrlich ascites carcinoma cells. J. Photochem. Photobiol. B 2006, 85, 163–176. [Google Scholar] [CrossRef]
  26. Qi, F.; Sun, Y.; Lv, M.; Qin, F.; Cao, W.; Bi, L. Effects of palmatine hydrochloride mediated photodynamic therapy on oral squamous cell carcinoma. Photochem. Photobiol. Sci. 2019, 18, 1596–1605. [Google Scholar] [CrossRef]
  27. Barrow, S.J.; Kasera, S.; Rowland, M.J.; Bamo, J.D.; Scheman, O.A. Cucurbituril-Based Molecular Recognition. Chem. Rev. 2015, 115, 12302–12406. [Google Scholar] [CrossRef]
  28. Swartjes, A.; White, P.B.; Lammertink, M.; Elemans, J.; Nolte, R. Host-Guest Exchange of viologen guests in porphyrin cage compounds as studied by selective exchange spectroscopy (1D EXSY) NMR. Angew. Chem. Int. Ed. Engl. 2021, 133, 1274–1282. [Google Scholar] [CrossRef]
  29. Wang, S.; Huang, Z.; Li, A.; Zhao, Y.; Zuo, W.; Li, Y.; Miao, H.; Ma, J.; Sun, W.; Wang, X.; et al. Crown Ether Functionalized Potassium-Responsive Anionocages for Cascaded Guest Delivery. Angew. Chem. Int. Ed. Engl. 2021, 60, 9573–9579. [Google Scholar] [CrossRef]
  30. Rebek, J., Jr. Host–guest chemistry of calixarene capsules. Chem. Commun. 2000, 8, 637–643. [Google Scholar] [CrossRef]
  31. Wankar, J.; Kotla, N.G.; Gera, S.; Rasala, S.; Pandit, A.; Rochev, Y.A. Recent Advances in Host–Guest Self-Assembled Cyclodextrin Carriers: Implications for Responsive Drug Delivery and Biomedical Engineering. Adv. Funct. Mater. 2020, 30, 1909049. [Google Scholar] [CrossRef]
  32. Abeysekera, A.M.; Sinha, A.S.; Aakeroy, C.B. The Impact of Halogen Substituents on the Synthesis and Structure of Co-Crystals of Pyridine Amides. Molecules 2021, 26, 1147. [Google Scholar] [CrossRef] [PubMed]
  33. Lou, Y.; Yu, K.; Wu, X.; Wang, Z.; Cui, Y.; Bao, H.; Wang, J.W.; Hu, X.R.; Ji, Y.X.; Tang, G. Co-Crystals of Resveratrol and Polydatin with L-Proline: Crystal Structures, Dissolution Properties, and In Vitro Cytotoxicities. Molecules 2021, 26, 5722. [Google Scholar] [CrossRef]
  34. Giliopoulos, D.; Zamboulis, A.; Giannakoudakis, D.; Bikiaris, D.; Triantafyllidis, K. Polymer/metal organic framework (MOF) nanocomposites for biomedical applications. Molecules 2020, 25, 185. [Google Scholar] [CrossRef]
  35. Jiang, X.; Yu, H.; Shi, J.; Bai, Q.; Xu, Y.; Zhang, Z.; Hao, X.Q.; Li, B.; Wang, P.; Wu, L.; et al. From Mechanically Interlocked Structures to Host–Guest Chemistry Based on Twisted Dimeric Architectures by Adjusting Space Constraints. CCS Chem. 2021, 3, 2042–2054. [Google Scholar] [CrossRef]
  36. Li, Y.; Tang, S.; Yusov, A.; Rose, J.; Borrfors, A.N.; Hu, C.T.; Ward, M.D. Hydrogen-bonded frameworks for molecular structure determination. Nat. Commun. 2019, 10, 4477. [Google Scholar] [CrossRef] [PubMed]
  37. Inokuma, Y.; Yoshioka, S.; Ariyoshi, J.; Arai, T.; Hitora, Y.; Takada, K.; Matsunaga, S.; Rissanen, K.; Fujita, M. X-ray analysis on the nanogram to microgram scale using porous complexes. Nature 2013, 495, 461–466. [Google Scholar] [CrossRef]
  38. Lee, S.; Kapustin, E.A.; Yaghi, O.M. Coordinative alignment of molecules in chiral metal-organic frameworks. Science 2016, 353, 808. [Google Scholar] [CrossRef]
  39. Chen, C.; Di, Z.; Li, H.; Liu, J.; Wu, M.; Hong, M. An ultrastable π–π Stacked Porous Organic Molecular Framework as a Crystalline Sponge for Rapid Molecular Structure Determination. CCS Chem. 2021, 3, 1352–1362. [Google Scholar] [CrossRef]
  40. Wei, D.G.; Wilson, W.D.; Neidle, S. Small-molecule binding to the DNA minor groove is mediated by a conserved water cluster. J. Am. Chem. Soc. 2013, 135, 1369–1377. [Google Scholar] [CrossRef] [Green Version]
Molecules 27 06179 g001 550
Figure 1. Schematic representation for the structural elucidation of natural products by HPLC/CC/SCD: (a) herbal crude extract; (b) HPLC analysis; (c) collection of individual peaks; (d) adding coformer and co-crystal growth; (e) single crystal diffraction data collection; and (f) elucidation of crystal structures.
Figure 1. Schematic representation for the structural elucidation of natural products by HPLC/CC/SCD: (a) herbal crude extract; (b) HPLC analysis; (c) collection of individual peaks; (d) adding coformer and co-crystal growth; (e) single crystal diffraction data collection; and (f) elucidation of crystal structures.
Molecules 27 06179 g001
Molecules 27 06179 sch001 550
Scheme 1. Synthesis of benzimidazole using CR raw material, total alkaloid, or individual alkaloid as catalysts. 1a: o-phenylenediamine, 1b: benzaldehyde, 1c: benzimidazole.
Scheme 1. Synthesis of benzimidazole using CR raw material, total alkaloid, or individual alkaloid as catalysts. 1a: o-phenylenediamine, 1b: benzaldehyde, 1c: benzimidazole.
Molecules 27 06179 sch001
Molecules 27 06179 g002 550
Figure 2. Coformer pre-screening: (a) HPLC chromatogram of the total alkaloid in CR (HPLC conditions; column: Phenomenex Luna C18 column (250 × 4.6 mm, 5 μm), mobile phase: 0.1% formic acid in water (A) acetonitrile (B); gradient profiles: 0–10 min, 5 → 15% B; 10–15 min, 15 → 25% B; 15–35 min, 25% B; flow rate: 1.0 mL/min, detection: UV at 278 nm); (b) HPLC of the precipitate obtained after adding NDS; (c) overlay of the chromatograms before (blue) and after (orange) adding NDS. The numbers 1-7 represent the major peaks in the HPLC chromatogram.
Figure 2. Coformer pre-screening: (a) HPLC chromatogram of the total alkaloid in CR (HPLC conditions; column: Phenomenex Luna C18 column (250 × 4.6 mm, 5 μm), mobile phase: 0.1% formic acid in water (A) acetonitrile (B); gradient profiles: 0–10 min, 5 → 15% B; 10–15 min, 15 → 25% B; 15–35 min, 25% B; flow rate: 1.0 mL/min, detection: UV at 278 nm); (b) HPLC of the precipitate obtained after adding NDS; (c) overlay of the chromatograms before (blue) and after (orange) adding NDS. The numbers 1-7 represent the major peaks in the HPLC chromatogram.
Molecules 27 06179 g002
Molecules 27 06179 g003 550
Figure 3. Precipitation formation in methanol solution after adding fumaric acid (i), succinic acid (ii), theophylline (iii), NDS (iv), and 2-naphthalenesulfonic acid (v).
Figure 3. Precipitation formation in methanol solution after adding fumaric acid (i), succinic acid (ii), theophylline (iii), NDS (iv), and 2-naphthalenesulfonic acid (v).
Molecules 27 06179 g003
Molecules 27 06179 g004 550
Figure 4. Inclusion complex of (NDS) ⊃ 12: (a) the 1H-NMR of magnoflorine (400 MHz, DMSO-d6) (I), magnoflorine + NDS (II) and NDS (III); (b) chemical structure of 1 with atom labels; (c) the molecular structure of magnoflorine (1) at 30% thermal ellipsoid with atom labelling scheme; (d) the asymmetric unit of (NDS) ⊃ 12, and the dashed lines show intermolecular hydrogen bonds, and (e) the packing diagram of (NDS) ⊃ 12 along the b-axis.
Figure 4. Inclusion complex of (NDS) ⊃ 12: (a) the 1H-NMR of magnoflorine (400 MHz, DMSO-d6) (I), magnoflorine + NDS (II) and NDS (III); (b) chemical structure of 1 with atom labels; (c) the molecular structure of magnoflorine (1) at 30% thermal ellipsoid with atom labelling scheme; (d) the asymmetric unit of (NDS) ⊃ 12, and the dashed lines show intermolecular hydrogen bonds, and (e) the packing diagram of (NDS) ⊃ 12 along the b-axis.
Molecules 27 06179 g004
Molecules 27 06179 g005 550
Figure 5. The X-ray analysis of compounds 2–7. 2: columbamine (peak 2); 3: jatrorrhizine (peak 3); 4: epiberberine (peak 4); 5: coptisine (peak 5); 6: palmatine (peak 6); and 7: berberine (peak 7). (a) Chemical structure; (b) 30% thermal ellipsoid; and (c) the asymmetric unit.
Figure 5. The X-ray analysis of compounds 2–7. 2: columbamine (peak 2); 3: jatrorrhizine (peak 3); 4: epiberberine (peak 4); 5: coptisine (peak 5); 6: palmatine (peak 6); and 7: berberine (peak 7). (a) Chemical structure; (b) 30% thermal ellipsoid; and (c) the asymmetric unit.
Molecules 27 06179 g005
Molecules 27 06179 g006 550
Figure 6. The packing diagram of inclusion complex: (a) (NDS) ⊃ 22 (observed along a-axis); (b) (NDS)1/2 ⊃ 3 (observed along b-axis); (c) (NDS)1/2 ⊃ 4 (observed along a-axis); (d) (NDS) ⊃ (5)2 (observed along b-axis); (e) (NDS)1/2 ⊃ 6 (observed along a-axis); and (f) (NDS)1/2 ⊃ 7 (observed along a-axis). a, b, and c with the arrows in each figure means the observation direction.
Figure 6. The packing diagram of inclusion complex: (a) (NDS) ⊃ 22 (observed along a-axis); (b) (NDS)1/2 ⊃ 3 (observed along b-axis); (c) (NDS)1/2 ⊃ 4 (observed along a-axis); (d) (NDS) ⊃ (5)2 (observed along b-axis); (e) (NDS)1/2 ⊃ 6 (observed along a-axis); and (f) (NDS)1/2 ⊃ 7 (observed along a-axis). a, b, and c with the arrows in each figure means the observation direction.
Molecules 27 06179 g006
Molecules 27 06179 g007 550
Figure 7. The calculated electrostatic potential surfaces. The red region shows the area with negative charge, while the blue region reveals the area with positive charge. (a) NDS and (b) magnoflorine (1). Note: a. u. means the electrostatic potential unit.
Figure 7. The calculated electrostatic potential surfaces. The red region shows the area with negative charge, while the blue region reveals the area with positive charge. (a) NDS and (b) magnoflorine (1). Note: a. u. means the electrostatic potential unit.
Molecules 27 06179 g007
Table
Table 1. Conditions and yields of the photocatalytic reaction a.
Table 1. Conditions and yields of the photocatalytic reaction a.
EntryPhotocatalyst (3%)Time (h)ConditionsYield b (%)
1CR2 h470 nmTrace
2 cCR12 h-Trace
3 d-12 h470 nmTrace
4CR12 h470 nm43.4
5Total alkaloids12 h470 nm58.2
6Magnolflorine (1)12 h470 nm54.6
7Columbamine (2)12 h470 nm82.1
8Jatrorrhizine (3)12 h470 nm73.3
9Epiberberine (4)12 h470 nm82.1
10Coptisine (5)12 h470 nm62.3
11Palmatine (6)12 h470 nm71.2
12Berberine (7)12 h470 nm73.6
a Reaction conditions: o-Phenylenediamine (0.1 mmol), benzaldehyde (0.1 mmol), photocatalyst (3%), 5 W blue LED and room temperature. b Yields calculated by HPLC peak area. c Without blue LED. d Without photocatalyst.
Table
Table 2. Changes of 1H-NMR chemical shifts of NDS in the NDS–alkaloid complex.
Table 2. Changes of 1H-NMR chemical shifts of NDS in the NDS–alkaloid complex.
δ/ppm NDSNDS ⊃ 12NDS ⊃ 22(NDS)1/2 ⊃ 3(NDS)1/2 ⊃ 4NDS ⊃ 52(NDS)1/2 ⊃ 6(NDS)1/2 ⊃ 7
NDSa7.987.93(−0.05)7.92(−0.06)7.92(−0.06)7.91(−0.07)7.92(−0.06)7.91(−0.07)7.92(−0.06)
b7.497.42(−0.07)7.41(−0.08)7.41(−0.08)7.41(−0.08)7.41(−0.08)7.39(−0.10)7.40(−0.09)
c8.888.87(−0.01)8.86(−0.02)8.86(−0.02)8.86(−0.02)8.86(−0.02)8.85(−0.03)8.86(−0.02)
Table
Table 3. Crystal data and refinement details of the co-crystals.
Table 3. Crystal data and refinement details of the co-crystals.
ItemsNDS ⊃ 12NDS ⊃ 22(NDS)1/2 ⊃ 3(NDS)1/2 ⊃ 4NDS ⊃ 52(NDS)1/2 ⊃ 6(NDS)1/2 ⊃ 7
FormulaC10H6O6S2,
2(C20H24NO4),
CH4O, 2(H2O)		C10H6O6S2,
2(C20H20NO4), 2(CH4O)		C5H3O3S,
C20H20NO4		C5H3O3S,
C20H18NO4		C10H6O6S2,
2(C19H14NO4)		C10H6O6S2, 2(C21H22NO4)C10H6O6S2, 2(C20H18NO4)
Mw [gmol−1]1039.141027.09481.50479.49926.89991.06479.49
Systemtriclinicmonoclinicmonoclinictriclinictriclinictriclinictriclinic
Space groupP1CcP21/nP-1P-1P-1P-1
a(Å)10.9669(7)12.0470(4)14.3561(5)9.2880(9)6.7985(3)7.3194(3)7.3159(4)
b(Å)11.0345(7)21.3470(6)7.1689(2)10.6480 (9)15.4959(8)12.5231(6)11.3649(6)
c(Å)12.7760(8)18.2115(5)21.2493(8)12.2878 (12)19.8126(9)14.2025(7)14.0441(8)
α(°)67.778(6)909076.056(8)94.084(4)103.042(4)66.555(5)
β(°)64.965(6)93.338(3)105.325(4)76.398(8)97.720(3)104.921(4)88.456(5)
γ(°)88.765(5)909088.900(8)91.639(4)106.169(4)77.854(5)
V(Å3)1278.9(1)4675.5(2)2109.1(1)1145.4(1)2061.5(1)1144.4(11045.2(1)
Z1442212
dcalcd [mg/cm3]1.349 1.459 1.5161.3901.4931.4381.524
Rint0.03190.03130.03800.04600.04060.02700.0728
GooF1.0331.0511.0541.0441.0161.0731.038
Temperature (K)150.00(10)150.00(10)150.00(10)150.00(10)150.00(10)150.00(10)150.00(10)
R1 [I > 2σ(I)]0.04360.04260.04590.04710.05500.04200.0624
wR2(all data)0.12510.11460.12330.12360.14730.11240.1772
F(000)550.02160.01008.0500.0960.0520.0250.0
CCDC No.2097760209776120977622097763209776420977652097766
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Qin, S.-Q.; Ma, J.; Wang, Q.-Q.; Xu, W.; Ye, W.-C.; Jiang, R.-W. Identification of Photocatalytic Alkaloids from Coptidis Rhizome by an Offline HPLC/CC/SCD Approach. Molecules 2022, 27, 6179. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules27196179

AMA Style

Qin S-Q, Ma J, Wang Q-Q, Xu W, Ye W-C, Jiang R-W. Identification of Photocatalytic Alkaloids from Coptidis Rhizome by an Offline HPLC/CC/SCD Approach. Molecules. 2022; 27(19):6179. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules27196179

Chicago/Turabian Style

Qin, Shu-Qin, Jun Ma, Qi-Qi Wang, Wei Xu, Wen-Cai Ye, and Ren-Wang Jiang. 2022. "Identification of Photocatalytic Alkaloids from Coptidis Rhizome by an Offline HPLC/CC/SCD Approach" Molecules 27, no. 19: 6179. https://0-doi-org.brum.beds.ac.uk/10.3390/molecules27196179

Article Metrics

Back to TopTop