Next Article in Journal
iNR-Drug: Predicting the Interaction of Drugs with Nuclear Receptors in Cellular Networking
Next Article in Special Issue
Investigation into Variation of Endogenous Metabolites in Bone Marrow Cells and Plasma in C3H/He Mice Exposed to Benzene
Previous Article in Journal
Comparative Molecular Dynamics Simulations of Mitogen-Activated Protein Kinase-Activated Protein Kinase 5
Previous Article in Special Issue
Mass Spectrometry Analysis Coupled with de novo Sequencing Reveals Amino Acid Substitutions in Nucleocapsid Protein from Influenza A Virus
Article

Phosphosite Mapping of HIP-55 Protein in Mammalian Cells

1
Institute of Vascular Medicine, Peking University Third Hospital, Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides, Ministry of Health, Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education and Beijing Key Laboratory of Cardiovascular Receptors Research, Beijing 100191, China
2
Central Laboratory, Jilin University Second Hospital, Changchun 130041, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2014, 15(3), 4903-4914; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms15034903
Received: 19 January 2014 / Revised: 20 February 2014 / Accepted: 7 March 2014 / Published: 19 March 2014
(This article belongs to the Special Issue Mass Spectrometry Application in Biology)
In the present study, hematopoietic progenitor kinase 1 (HPK1)-interacting protein of 55 kDa (HIP-55) protein was over-expressed in HEK293 cells, which was genetically attached with 6x His tag. The protein was purified by nickel-charged resin and was then subjected to tryptic digestion. The phosphorylated peptides within the HIP-55 protein were enriched by TiO2 affinity chromatography, followed by mass spectrometry analysis. Fourteen phosphorylation sites along the primary structure of HIP-55 protein were identified, most of which had not been previously reported. Our results indicate that bio-mass spectrometry coupled with manual interpretation can be used to successfully identify the phosphorylation modification in HIP-55 protein in HEK293 cells. View Full-Text
Keywords: mass spectrometry; HIP-55; phosphorylation mass spectrometry; HIP-55; phosphorylation
Show Figures

MDPI and ACS Style

Liu, N.; Sun, N.; Gao, X.; Li, Z. Phosphosite Mapping of HIP-55 Protein in Mammalian Cells. Int. J. Mol. Sci. 2014, 15, 4903-4914. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms15034903

AMA Style

Liu N, Sun N, Gao X, Li Z. Phosphosite Mapping of HIP-55 Protein in Mammalian Cells. International Journal of Molecular Sciences. 2014; 15(3):4903-4914. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms15034903

Chicago/Turabian Style

Liu, Ning, Ningning Sun, Xiang Gao, and Zijian Li. 2014. "Phosphosite Mapping of HIP-55 Protein in Mammalian Cells" International Journal of Molecular Sciences 15, no. 3: 4903-4914. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms15034903

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop