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Article

Oil Body-Bound Oleosin-rhFGF-10: A Novel Drug Delivery System that Improves Skin Penetration to Accelerate Wound Healing and Hair Growth in Mice

1
College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China
2
Wenzhou Biomedical Innovation Center, Wenzhou University, Wenzhou 325035, China
3
College of Life and Environmental Sciences, Wenzhou University, Wenzhou 325035, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2017, 18(10), 2177; https://doi.org/10.3390/ijms18102177
Received: 25 September 2017 / Revised: 13 October 2017 / Accepted: 15 October 2017 / Published: 18 October 2017
(This article belongs to the Special Issue Guided Amphiphile and Lipid Membrane Self-Assembly)
Recombinant human fibroblast growth factor 10 (rhFGF-10) is frequently used to treat patients with skin injuries. It can also promote hair growth. However, the effective application of rhFGF-10 is limited because of its poor stability and transdermal absorption. In this study, polymerase chain reaction (PCR) and Southern blotting were used to identify transgenic safflowers carrying a gene encoding an oleosin-rhFGF-10 fusion protein. The size and structural integrity of oleosin-rhFGF-10 in oil bodies extracted from transgenic safflower seeds was characterized by polyacrylamide gel electrophoresis and western blotting. Oil body extracts containing oleosin-rhFGF-10 were topically applied to mouse skin. The absorption of oleosin-rhFGF-10 was studied by immunohistochemistry. Its efficiency in promoting wound healing and hair regeneration were evaluated in full thickness wounds and hair growth assays. We identified a safflower line that carried the transgene and expressed a 45 kDa oleosin-rhFGF-10 protein. Oil body-bound oleosin-rhFGF-10 was absorbed by the skin with higher efficiency and speed compared with prokaryotically-expressed rhFGF-10. Oleosin-rhFGF-10 also enhanced wound closure and promoted hair growth better than rhFGF-10. The application of oleosin-rhFGF-10 in oil bodies promoted its delivery through the skin, providing a basis for improved therapeutic effects in enhancing wound healing and hair growth. View Full-Text
Keywords: oil body; oleosin-rhFGF-10; skin penetration; wound healing; hair growth oil body; oleosin-rhFGF-10; skin penetration; wound healing; hair growth
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MDPI and ACS Style

Li, W.; Yang, J.; Cai, J.; Wang, H.; Tian, H.; Huang, J.; Qiang, W.; Zhang, L.; Li, H.; Li, X.; Jiang, C. Oil Body-Bound Oleosin-rhFGF-10: A Novel Drug Delivery System that Improves Skin Penetration to Accelerate Wound Healing and Hair Growth in Mice. Int. J. Mol. Sci. 2017, 18, 2177. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18102177

AMA Style

Li W, Yang J, Cai J, Wang H, Tian H, Huang J, Qiang W, Zhang L, Li H, Li X, Jiang C. Oil Body-Bound Oleosin-rhFGF-10: A Novel Drug Delivery System that Improves Skin Penetration to Accelerate Wound Healing and Hair Growth in Mice. International Journal of Molecular Sciences. 2017; 18(10):2177. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18102177

Chicago/Turabian Style

Li, Wenqing, Jing Yang, Jingbo Cai, Hongyu Wang, Haishan Tian, Jian Huang, Weidong Qiang, Linbo Zhang, Haiyan Li, Xiaokun Li, and Chao Jiang. 2017. "Oil Body-Bound Oleosin-rhFGF-10: A Novel Drug Delivery System that Improves Skin Penetration to Accelerate Wound Healing and Hair Growth in Mice" International Journal of Molecular Sciences 18, no. 10: 2177. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18102177

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