Exploring the Functions of 9-Lipoxygenase (DkLOX3) in Ultrastructural Changes and Hormonal Stress Response during Persimmon Fruit Storage
Abstract
:1. Introduction
2. Results
2.1. Physiological Characterization during Persimmon Fruit Storage
2.2. Structural Analyses of the Persimmon Fruit Peel during Storage
2.3. Immunocytolocalization of DkLOX3 in Persimmon Fruit during Storage
2.4. Expression of DkLOX3 during Persimmon Fruit Storage
2.5. Isolation and Sequence Analysis of the DkLOX3 Promoter
2.6. Characterization of DkLOX3 Promoter Activity in Tobacco Leaves
2.7. Responsiveness of DkLOX3 Promoters to Hormonal Stress
3. Discussion
3.1. DkLOX3 May Have a Positive Role in Ultrastructural Changes during Fruit Ripening
3.2. DkLOX3 May Play an Important Role in Hormonal Stress Response during Fruit Ripening
4. Materials and Methods
4.1. Plant Materials and Treatments
4.2. Fruit Firmness, Ethylene Production and Weight Loss
4.3. Structural Analyses
4.4. Immunolabelling for Transmission Electron Microscopy (TEM)
4.5. Quantitative Real-Time PCR Analysis
4.6. Cloning of the 5′ Flanking Region of DkLOX3
4.7. Construction of the Promoter-GUS Fusion
4.8. Agrobacterium-Mediated Transient Expression Assays in Tobacco Plants
4.9. Histochemical and Fluorometric Assays for GUS Activity
4.10. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Primer Name | Primer Sequence (5′–3′) | Purpose |
---|---|---|
pLOX3-SP1 | AACTCAGTGTGGTGAAGATTGCGGATG | |
pLOX3-SP2 | AAGGCGACATCGAAAGCTGAATCTCC | DkLOX3 promoter clone |
pLOX3-SP3 | CAAGTAAGCTGCCTTCCCAAGCTTCC | |
DkLOX3-PF | CCCAAGCTTCCACAATTATTCTTTGGTTATTTCG | DkLOX3 full-length promoter clone |
DkLOX3-PR | GCTCTAGACTTCTTCTTCTTCTTCTTCTTCTGC | |
DkLOX3-PF5 | CCCAAGCTTTATAGTATGGTCTATCATTCTCATACG | DkLOX3 promoter deletion derivatives construct |
DkLOX3-PF4 | CCCAAGCTTGTACCCCATGTTTGATACAACTCT | |
DkLOX3-PF3 | CCCAAGCTTGCATTTCTATGTATGCTCTCTTATG | |
DkLOX3-PF2 | CCCAAGCTTCTGGATCTAGTTCAACCCATAAG | |
DkLOX3-PF1 | CCCAAGCTTTTATCAGGGATCTGATTTGTCTTA | |
DkLOX3qF | CACTGCTCTTCCCTACCA | DkLOX3 qRT-PCR |
DkLOX3qR | CAGAGGGAGAAATCAGTGATACAC | |
ActinqF | GGATTCTGGTGATGGTGTTAG | Actin qRT-PCR |
ActinqR | CAGCAGTTGTTGTGAAGGAGT |
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Meng, K.; Hou, Y.; Han, Y.; Ban, Q.; He, Y.; Suo, J.; Rao, J. Exploring the Functions of 9-Lipoxygenase (DkLOX3) in Ultrastructural Changes and Hormonal Stress Response during Persimmon Fruit Storage. Int. J. Mol. Sci. 2017, 18, 589. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18030589
Meng K, Hou Y, Han Y, Ban Q, He Y, Suo J, Rao J. Exploring the Functions of 9-Lipoxygenase (DkLOX3) in Ultrastructural Changes and Hormonal Stress Response during Persimmon Fruit Storage. International Journal of Molecular Sciences. 2017; 18(3):589. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18030589
Chicago/Turabian StyleMeng, Kun, Yali Hou, Ye Han, Qiuyan Ban, Yiheng He, Jiangtao Suo, and Jingping Rao. 2017. "Exploring the Functions of 9-Lipoxygenase (DkLOX3) in Ultrastructural Changes and Hormonal Stress Response during Persimmon Fruit Storage" International Journal of Molecular Sciences 18, no. 3: 589. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms18030589