Conditions Inducing Excessive O-GlcNAcylation Inhibit BMP2-Induced Osteogenic Differentiation of C2C12 Cells
Abstract
:1. Introduction
2. Results
2.1. Excessive O-GlcNAcylation-Inducing Conditions Suppressed BMP2-Induced ALP Activity and Osteogenic Marker Gene Expression
2.2. Inhibition of OGT Activity Rescued BMP2-Induced Osteogenic Differentiation that was Downregulated by O-GlcNAcylation Inducers
2.3. OGT Overexpression Inhibited BMP2-Induced Osteogenic Differentiation, whereas OGA Overexpression Rescued N-Acetylglucosamine-Inhibited Osteogenic Differentiation
2.4. Excessive O-GlcNAcylation Suppressed Runx2 Transcriptional Activity, Which Was Restored by OGT Inhibitor Addition or OGA Overexpression
2.5. Excessive O-GlcNAcylation Attenuated Osteogenic Differentiation and Matrix Mineralization of Human Periodontal Ligament Cells
3. Discussion
4. Materials and Methods
4.1. Cell Cultures and Reagents
4.2. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) Analysis
4.3. Western Blot Analysis and Immunoprecipitation
4.4. ALP Staining, ALP Activity Assays, and Alizarin Red S Staining
4.5. Luciferase Reporter Assays
4.6. Statistical Analysis
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Gu, H.; Song, M.; Boonanantanasarn, K.; Baek, K.; Woo, K.M.; Ryoo, H.-M.; Baek, J.-H. Conditions Inducing Excessive O-GlcNAcylation Inhibit BMP2-Induced Osteogenic Differentiation of C2C12 Cells. Int. J. Mol. Sci. 2018, 19, 202. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms19010202
Gu H, Song M, Boonanantanasarn K, Baek K, Woo KM, Ryoo H-M, Baek J-H. Conditions Inducing Excessive O-GlcNAcylation Inhibit BMP2-Induced Osteogenic Differentiation of C2C12 Cells. International Journal of Molecular Sciences. 2018; 19(1):202. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms19010202
Chicago/Turabian StyleGu, Hanna, Mina Song, Kanitsak Boonanantanasarn, Kyunghwa Baek, Kyung Mi Woo, Hyun-Mo Ryoo, and Jeong-Hwa Baek. 2018. "Conditions Inducing Excessive O-GlcNAcylation Inhibit BMP2-Induced Osteogenic Differentiation of C2C12 Cells" International Journal of Molecular Sciences 19, no. 1: 202. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms19010202