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Article

Mouse Tryptase Gene Expression is Coordinately Regulated by GATA1 and GATA2 in Bone Marrow-Derived Mast Cells

1
Department of Pharmacy, Faculty of Pharmacy, Takasaki University of Health and Welfare, Takasaki 370-0033, Japan
2
Department of Medical Biochemistry, Tohoku University Graduate School of Medicine, Sendai 980-8573, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(18), 4603; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20184603
Received: 16 July 2019 / Revised: 9 September 2019 / Accepted: 11 September 2019 / Published: 17 September 2019
(This article belongs to the Special Issue Activation and Modulation of Mast Cells)
Mast cell tryptases have crucial roles in allergic and inflammatory diseases. The mouse tryptase genes represent a cluster of loci on chromosome 16p3.3. While their functional studies have been extensively performed, transcriptional regulation of tryptase genes is poorly understood. In this study, we examined the molecular basis of the tryptase gene expression in bone marrow-derived mast cells (BMMCs) of C57BL/6 mice and in MEDMC-BRC6 mast cells. The expression of the Tpsb2 and Tpsg1 genes, which reside at the 3′-end of the tryptase locus, is significantly decreased by the reduction of the GATA transcription factors GATA1 or GATA2. Chromatin immunoprecipitation assays have shown that the GATA factors bind at multiple regions within the locus, including 1.0 and 72.8 kb upstream of the Tpsb2 gene, and that GATA1 and GATA2 facilitate each other’s DNA binding activity to these regions. Deletion of the −72.8 kb region by genome editing significantly reduced the Tpsb2 and Tpsg1 mRNA levels in MEDMC-BRC6 cells. Furthermore, binding of CTCF and the cohesin subunit Rad21 was found upstream of the −72.8 kb region and was significantly reduced in the absence of GATA1. These results suggest that mouse tryptase gene expression is coordinately regulated by GATA1 and GATA2 in BMMCs. View Full-Text
Keywords: mast cell; tryptase; gene transcription mast cell; tryptase; gene transcription
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MDPI and ACS Style

Ohneda, K.; Ohmori, S.; Yamamoto, M. Mouse Tryptase Gene Expression is Coordinately Regulated by GATA1 and GATA2 in Bone Marrow-Derived Mast Cells. Int. J. Mol. Sci. 2019, 20, 4603. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20184603

AMA Style

Ohneda K, Ohmori S, Yamamoto M. Mouse Tryptase Gene Expression is Coordinately Regulated by GATA1 and GATA2 in Bone Marrow-Derived Mast Cells. International Journal of Molecular Sciences. 2019; 20(18):4603. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20184603

Chicago/Turabian Style

Ohneda, Kinuko, Shin’ya Ohmori, and Masayuki Yamamoto. 2019. "Mouse Tryptase Gene Expression is Coordinately Regulated by GATA1 and GATA2 in Bone Marrow-Derived Mast Cells" International Journal of Molecular Sciences 20, no. 18: 4603. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20184603

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