Differences in Expression of Human Leukocyte Antigen Class II Subtypes and T Cell Subsets in Behçet’s Disease with Arthritis
Abstract
:1. Introduction
2. Results
2.1. Clinical Characteristics of Patients
2.2. Frequencies of HLA-DP, DQ, and DR Positive Cells in Active BD Patients
2.3. Differential Frequencies of CCR7+ Cells between Active BD and Healthy Controls
2.4. Differential Frequencies of Memory T Cells between Active and Inactive BD Patients
2.5. Correlation between Frequencies of HLA Class II Subtypes Expressing Cells and Disease Activity Markers in BD Patients
2.6. Correlation between Frequencies of CCR7 Expressing Cells and Disease Activity Markers in BD Patients
3. Discussion
4. Materials and Methods
4.1. Patients
4.2. Flow Cytometry Analysis of Patients’ Blood Samples
4.3. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Patients | Age | OU | GU | Arth | EN | Disease Severity Score | Leukocyte | ESR | CRP | ||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1st | 2nd | 1st | 2nd | 1st | 2nd | 1st | 2nd | ||||||
N = 25 (M = 5, F = 20) | 48.8 ± 7.6 | 13 (52.0%) | 6 (24.0%) | 25 (100%) | 7 (28.0%) | 3.04 ± 1.1 | 1.27 ± 1.0 | 8008.0 ± 3480.1 | 6836.4 ± 1937.1 | 29.8 ± 25.8 | 18.3 ±13.6 | 1.01 ± 2.02 | 0.26 ± 0.37 |
P value | P = 0.007 | P = 0.168 | P = 0.083 | P = 0.314 |
Ordrer | Colchicine | Steroid | AZP | Bucillamine | HCQ | SZP | NSAIDs | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1st | 2nd | 1st | 2nd | 1st | 2nd | 1st | 2nd | 1st | 2nd | 1st | 2nd | 1st | 2nd | |
Number (%) | 19 (76.2%) | 9 (81.8%) | 21 (84.0%) | 7 (63.6%) | 7 (28.0%) | 3 (27.3%) | 1 (8.0%) | 2 (18.2%) | 8 (32.0%) | 4 (36.4%) | 10 (40.0%) | 5 (45.5%) | 16 (64.0%) | 7 (63.6%) |
Disease Activity Markers | Correlation Coefficient, r (p-value) | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
HLA-DP | HLA-DQ | HLA-DR | |||||||||||
PBL | Monocyte | Lymphocyte | Granulocyte | PBL | Monocyte | Lymphocyte | Granulocyte | PBL | Monocyte | Lymphocyte | Granulocyte | ||
Disease severity score | −0.015 (0.945) | 0.202 (0.334) | 0.138 (0.510) | 0.193 (0.355) | −0.033 (0.876) | 0.365 (0.073) | 0.091 (0.665) | −0.088 (0.677) | −0.004 (0.984) | 0.379 (0.062) | 0.374 (0.066) | −0.041 (0.847) | |
Leukocyte number | 0.025 (0.907) | −0.226 (0.278) | −0.169 (0.419) | 0.123 (0.557) | −0.021 (0.919) | −0.323 (0.115) | −0.394 (0.051) | 0.364 (0.073) | 0.218 (0.294) | −0.104 (0.622) | −0.117 (0.579) | 0.289 (0.161) | |
ESR | −0.142 (0.498) | 0.044 (0.834) | −0.289 (0.161) | 0.180 (0.389) | −0.225 (0.279) | 0.055 (0.792) | 0.010 (0.964) | −0.230 (0.269) | −0.33 (0.059) | 0.107 (0.610) | 0.060 (0.777) | −0.034 (0.872) | |
CRP | −0.179 (0.393) | 0.213 (0.306) | −0.289 (0.161) | 0.001 (0.996) | −0.438 (0.029) | −0.213 (0.307) | −0.290 (0.160) | −0.078 (0.709) | −0.234 (0.260) | 0.385 (0.058) | −0.005 (0.982) | −0.062 (0.769) |
Disease Activity Markers | Correlation Coefficient, r (p-value) | ||||||
---|---|---|---|---|---|---|---|
CD4+ | CD8+ | CCR7+ | CD4+CCR7+ | CD8+CCR7+ | CD4+CD62L- | CD8+CD62L- | |
Leukocyte number | 0.316 (0.124) | 0.378 (0.063) | 0.431 (0.031) | 0.351 (0.086) | 0.417 (0.038) | 0.325 (0.113) | 0.266 (0.198) |
ESR | −0.382 (0.060) | −0.376 (0.064) | −0.258 (0.213) | −0.385 (0.057) | −0.391 (0.053) | −0.267 (0.197) | −0.339 (0.097) |
CRP | −0.161 (0.442) | −0.198 (0.342) | −0.119 (0.572) | −0.187 (0.370) | −0.184 (0.380) | −0.063 (0.764) | −0.109 (0.603) |
Correlation Coefficient, r (p-value) | ||||||
---|---|---|---|---|---|---|
CD4+ Naïve T | CD4+ Effector Memory T | CD4+ Central Memory T | CD8+ Naïve T | CD8+ Effector Memory T | CD8+ Central Memory T | |
Disease Severity Score | 0.259 (0.212) | −0.358 (0.079) | 0.075 (0.723) | 0.463 (0.020) | −0.214 (0.305) | 0.163 (0.435) |
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Islam, S.M.S.; Kim, H.-A.; Choi, B.; Jung, J.-Y.; Lee, S.-M.; Suh, C.-H.; Sohn, S. Differences in Expression of Human Leukocyte Antigen Class II Subtypes and T Cell Subsets in Behçet’s Disease with Arthritis. Int. J. Mol. Sci. 2019, 20, 5044. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20205044
Islam SMS, Kim H-A, Choi B, Jung J-Y, Lee S-M, Suh C-H, Sohn S. Differences in Expression of Human Leukocyte Antigen Class II Subtypes and T Cell Subsets in Behçet’s Disease with Arthritis. International Journal of Molecular Sciences. 2019; 20(20):5044. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20205044
Chicago/Turabian StyleIslam, S. M. Shamsul, Hyoun-Ah Kim, Bunsoon Choi, Ju-Yang Jung, Sung-Min Lee, Chang-Hee Suh, and Seonghyang Sohn. 2019. "Differences in Expression of Human Leukocyte Antigen Class II Subtypes and T Cell Subsets in Behçet’s Disease with Arthritis" International Journal of Molecular Sciences 20, no. 20: 5044. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20205044