Parallel Bud Mutation Sequencing Reveals that Fruit Sugar and Acid Metabolism Potentially Influence Stress in Malus
Abstract
:1. Introduction
2. Results
2.1. Distinct Components of Sugar and Acid Between Jonathan and Sweet Jonathan during Fruit Development
2.2. Whole Genome Sequencing of Jonathan and Sweet Jonathan Enables Identification of Extensive Genetic Mutations between Jonathan and Sweet Jonathan
2.3. The Transcriptome Profiles of Jonatha’ and Sweet Jonathan during Fruit Development
2.4. Identification of Co-Expression Modules
2.5. Deciphering Key Co-Expression Modules
2.6. Sugar Metabolism and Accumulation in Apple Fruit Development
2.7. The Stop Gain Mutation in MdMa1 may Partly Explain the Low Acid Content in Sweet Jonathan
2.8. Dramatic Upregulation of ABC Transporter Genes in Sweet Jonathan is Potentially Regulated by MdBPC6
2.9. PlantHhormone Signal Transduction
2.10. Disease Resistance Protein Genes
3. Discussion
3.1. Huge Numbers of Mutations Identified by Whole Genome Resequencing Suggested Variable Levels of Genetic Diversity among Apple Bud Mutant Cultivars
3.2. Identifying Hub Genes Associated with Sugar and Acid Accumulation by Deciphering Co-Expression Modules
3.3. Sugar and Acid in Apple Fruit may Influence Stress during Fruit Development
3.4. TranscriptFfactor MdBCP6 Could Regulate ABC Transporter Genes and Potentially Participate in Fruit Development or Stress Response
4. Materials and Methods
4.1. Plant Materials and Sample Collection
4.2. Measurement of Sugars and Acid
4.3. Resequencing of Jonathan and Sweet Jonathan
4.4. RNA-Seq Library Construction, Sequencing, and Data Processing
4.5. Identification of Co-Expression Modules
4.6. Visualization of Hub Genes
4.7. Gene Expression Analysis
4.8. Validation of SNPs and InDels
4.9. Identification of Cis-Motifs
4.10. Yeast One-Hybrid Assay
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Zhao, J.; Shen, F.; Gao, Y.; Wang, D.; Wang, K. Parallel Bud Mutation Sequencing Reveals that Fruit Sugar and Acid Metabolism Potentially Influence Stress in Malus. Int. J. Mol. Sci. 2019, 20, 5988. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20235988
Zhao J, Shen F, Gao Y, Wang D, Wang K. Parallel Bud Mutation Sequencing Reveals that Fruit Sugar and Acid Metabolism Potentially Influence Stress in Malus. International Journal of Molecular Sciences. 2019; 20(23):5988. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20235988
Chicago/Turabian StyleZhao, Jirong, Fei Shen, Yuan Gao, Dajiang Wang, and Kun Wang. 2019. "Parallel Bud Mutation Sequencing Reveals that Fruit Sugar and Acid Metabolism Potentially Influence Stress in Malus" International Journal of Molecular Sciences 20, no. 23: 5988. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20235988