Bergenin Reduces Experimental Painful Diabetic Neuropathy by Restoring Redox and Immune Homeostasis in the Nervous System
Abstract
:1. Introduction
2. Results
2.1. Acute Bergenin Treatment Ameliorates Established Painful Diabetic Neuropathy
2.2. Multiple Doses of Bergenin Revert Behavioral Painful Diabetic Neuropathy
2.3. Bergenin Treatment Does Not Reduce Glycemia in Diabetic Mice
2.4. Bergenin Reduces NO Production in Vitro
2.5. Bergenin Reduces Markers of Oxidative Stress in Nervous System of Neuropathic Mice
2.6. Bergenin Modifies the Antioxidant Profile in the Nervous System of Mice with Painful DN
2.7. Bergenin Modulates Pro- and Anti-Inflammatory Cytokines Production in the Nervous System
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Bergenin
4.3. Mouse Model of STZ-Induced Diabetes
4.4. Experimental Design
4.5. Assessment of Sensory Neuropathy by Von Frey Filaments
4.6. The Rota-Rod Test for Assessment of Motor Impairment
4.7. In Vitro Assays: Cytotoxicity and Nitrite Levels in Macrophage Cultures
4.8. Estimation of Lipid Peroxidation and Nitrite in Nervous Tissues
4.9. Real-Time PCR
4.10. Quantification of Cytokines Levels by ELISA
4.11. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
AGE | Advanced glycation end product |
BER | Bergenin |
CTRL | Control |
DEXA | Dexamethasone |
DMEM | Dulbecco’s modified Eagle medium |
DMSO | Dimethyl sulfoxide |
DN | Diabetic neuropathy |
DRG | Dorsal root ganglia |
ELISA | Enzyme-linked immunosorbent assay |
FBS | Fetal bovine serum |
GABA | Gabapentin |
Gpx | Glutathione peroxidase |
GV | Gentian violet |
IFN-γ | Interferon-γ |
IL-1β | Interleukin-1β |
iNOS | Inducible nitric oxide synthase |
LPS | Lipopolysaccharide |
MDA | Malondialdehyde |
NF-κB | Nuclear factor kappa-light-chain-enhancer of activated B cells |
NO | Nitric oxide |
Nrf2 | Nuclear factor erythroid 2-related factor 2 |
PBS | Phosphate buffered saline |
ROS | Reactive oxygen species |
RT-qPCR | Real-time quantitative polymerase chain reaction |
STZ | Streptozotocin |
TBARS | Thiobarbituric acid-reactive substances |
TGF-β | Transforming growth factor-β |
TNF-α | Tumor necrosis factor-α |
VEH | Vehicle |
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Villarreal, C.F.; Santos, D.S.; Lauria, P.S.S.; Gama, K.B.; Espírito-Santo, R.F.; Juiz, P.J.L.; Alves, C.Q.; David, J.M.; Soares, M.B.P. Bergenin Reduces Experimental Painful Diabetic Neuropathy by Restoring Redox and Immune Homeostasis in the Nervous System. Int. J. Mol. Sci. 2020, 21, 4850. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21144850
Villarreal CF, Santos DS, Lauria PSS, Gama KB, Espírito-Santo RF, Juiz PJL, Alves CQ, David JM, Soares MBP. Bergenin Reduces Experimental Painful Diabetic Neuropathy by Restoring Redox and Immune Homeostasis in the Nervous System. International Journal of Molecular Sciences. 2020; 21(14):4850. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21144850
Chicago/Turabian StyleVillarreal, Cristiane F., Dourivaldo S. Santos, Pedro S. S. Lauria, Kelly B. Gama, Renan F. Espírito-Santo, Paulo J. L. Juiz, Clayton Q. Alves, Jorge M. David, and Milena B. P. Soares. 2020. "Bergenin Reduces Experimental Painful Diabetic Neuropathy by Restoring Redox and Immune Homeostasis in the Nervous System" International Journal of Molecular Sciences 21, no. 14: 4850. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21144850