A DNA Aptameric Ligand of Human Transferrin Receptor Generated by Cell-SELEX
Abstract
:1. Introduction
2. Results and Discussion
2.1. Selection of Aptamers against Malignant Tumor Cells
2.2. Characterization and Optimization of Selected Aptamers
2.3. Identification of Target Protein of Aptamer HG1-9
2.4. Comparison of Natural Ligand hTf and Aptamer HG1-9
2.5. The Transcytosis of Aptamer HG1-9 across Epithelium Barrier
3. Materials and Methods
3.1. Materials
3.2. Cell Lines and Cell Culture
3.3. Procedures of Cell-SELEX
3.4. Flow Cytometry Analysis
3.5. Confocal Microscopy Imaging
3.6. Proteinase Digestion
3.7. SILAC-Based Proteomic Assay for Target Protein Identification
3.8. siRNA Transfection
3.9. Investigation of the Transcytosis of hTf and HG1-9 by an Epithelium Model Established by Caco-2 Cells
3.10. Statistical Analysis
4. Conclusions
5. Patents
Supplementary Materials
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhang, N.; Bing, T.; Shen, L.; Feng, L.; Liu, X.; Shangguan, D. A DNA Aptameric Ligand of Human Transferrin Receptor Generated by Cell-SELEX. Int. J. Mol. Sci. 2021, 22, 8923. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22168923
Zhang N, Bing T, Shen L, Feng L, Liu X, Shangguan D. A DNA Aptameric Ligand of Human Transferrin Receptor Generated by Cell-SELEX. International Journal of Molecular Sciences. 2021; 22(16):8923. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22168923
Chicago/Turabian StyleZhang, Nan, Tao Bing, Luyao Shen, Le Feng, Xiangjun Liu, and Dihua Shangguan. 2021. "A DNA Aptameric Ligand of Human Transferrin Receptor Generated by Cell-SELEX" International Journal of Molecular Sciences 22, no. 16: 8923. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22168923