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Article

Patient-Derived Human Basal and Cutaneous Squamous Cell Carcinoma Tissues Display Apoptosis and Immunomodulation following Gas Plasma Exposure with a Certified Argon Jet

1
ZIK Plasmatis, Leibniz Institute for Plasma Science and Technology (INP), Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany
2
Department of Oral and Maxillofacial Surgery, Plastic Surgery, Greifswald University Medical Center, Ferdinand-Sauerbruch-Str., 17475 Greifswald, Germany
3
Department of General, Visceral, Thoracic and Vascular Surgery, Greifswald University Medical Center, Ferdinand-Sauerbruch-Str., 17475 Greifswald, Germany
4
Departments of Dermato-Oncology, Essen/Duisburg University Medical Center, Hufelandstr. 55, 45147 Essen, Germany
5
West-German Cancer Center, Essen/Duisburg University Medical Center, Hufelandstr. 55, 45147 Essen, Germany
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German Consortium for Translational Cancer Research, Essen/Duisburg University Medical Center, Hufelandstr. 55, 45147 Essen, Germany
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Department of Dermatology and Allergology of the Ludwig Maximilian University Hospital Munich, 80337 Munich, Germany
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Clinic for Dermatology and Venerology, Rostock University Medical Center, Strempelstr. 13, 18057 Rostock, Germany
*
Author to whom correspondence should be addressed.
Academic Editors: Akikazu Sakudo and Yoshihito Yagyu
Int. J. Mol. Sci. 2021, 22(21), 11446; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222111446
Received: 4 September 2021 / Revised: 20 October 2021 / Accepted: 21 October 2021 / Published: 23 October 2021
(This article belongs to the Special Issue Plasma Biology 2.0)
Reactive oxygen species (ROS) have been subject of increasing interest in the pathophysiology and therapy of cancers in recent years. In skin cancer, ROS are involved in UV-induced tumorigenesis and its targeted treatment via, e.g., photodynamic therapy. Another recent technology for topical ROS generation is cold physical plasma, a partially ionized gas expelling dozens of reactive species onto its treatment target. Gas plasma technology is accredited for its wound-healing abilities in Europe, and current clinical evidence suggests that it may have beneficial effects against actinic keratosis. Since the concept of hormesis dictates that low ROS levels perform signaling functions, while high ROS levels cause damage, we investigated herein the antitumor activity of gas plasma in non-melanoma skin cancer. In vitro, gas plasma exposure diminished the metabolic activity, preferentially in squamous cell carcinoma cell (SCC) lines compared to non-malignant HaCaT cells. In patient-derived basal cell carcinoma (BCC) and SCC samples treated with gas plasma ex vivo, increased apoptosis was found in both cancer types. Moreover, the immunomodulatory actions of gas plasma treatment were found affecting, e.g., the expression of CD86 and the number of regulatory T-cells. The supernatants of these ex vivo cultured tumors were quantitatively screened for cytokines, chemokines, and growth factors, identifying CCL5 and GM-CSF, molecules associated with skin cancer metastasis, to be markedly decreased. These findings suggest gas plasma treatment to be an interesting future technology for non-melanoma skin cancer topical therapy. View Full-Text
Keywords: chemokines; cold physical plasma; cytokines; reactive oxygen species; ROS; skin cancer chemokines; cold physical plasma; cytokines; reactive oxygen species; ROS; skin cancer
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MDPI and ACS Style

Saadati, F.; Moritz, J.; Berner, J.; Freund, E.; Miebach, L.; Helfrich, I.; Stoffels, I.; Emmert, S.; Bekeschus, S. Patient-Derived Human Basal and Cutaneous Squamous Cell Carcinoma Tissues Display Apoptosis and Immunomodulation following Gas Plasma Exposure with a Certified Argon Jet. Int. J. Mol. Sci. 2021, 22, 11446. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222111446

AMA Style

Saadati F, Moritz J, Berner J, Freund E, Miebach L, Helfrich I, Stoffels I, Emmert S, Bekeschus S. Patient-Derived Human Basal and Cutaneous Squamous Cell Carcinoma Tissues Display Apoptosis and Immunomodulation following Gas Plasma Exposure with a Certified Argon Jet. International Journal of Molecular Sciences. 2021; 22(21):11446. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222111446

Chicago/Turabian Style

Saadati, Fariba, Juliane Moritz, Julia Berner, Eric Freund, Lea Miebach, Iris Helfrich, Ingo Stoffels, Steffen Emmert, and Sander Bekeschus. 2021. "Patient-Derived Human Basal and Cutaneous Squamous Cell Carcinoma Tissues Display Apoptosis and Immunomodulation following Gas Plasma Exposure with a Certified Argon Jet" International Journal of Molecular Sciences 22, no. 21: 11446. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222111446

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