CD93 Signaling via Rho Proteins Drives Cytoskeletal Remodeling in Spreading Endothelial Cells
Abstract
:1. Introduction
2. Results
2.1. CD93 Modulates Activation and Localization of Src Kinase
2.2. CD93 Controls Phosphorylation and Cellular Localization of Cbl
2.3. CD93 Regulates Activity of Rho-Proteins along the Cell Edge of Spreading ECs
3. Discussion
4. Materials and Methods
4.1. DNA Constructs and RNA Interference
4.2. Cell Culture
4.3. Antibodies and Reagents
4.4. Western Blotting Analyses
4.5. Immunofluorescent and FRET Analyses
4.6. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Barbera, S.; Raucci, L.; Lugano, R.; Tosi, G.M.; Dimberg, A.; Santucci, A.; Galvagni, F.; Orlandini, M. CD93 Signaling via Rho Proteins Drives Cytoskeletal Remodeling in Spreading Endothelial Cells. Int. J. Mol. Sci. 2021, 22, 12417. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222212417
Barbera S, Raucci L, Lugano R, Tosi GM, Dimberg A, Santucci A, Galvagni F, Orlandini M. CD93 Signaling via Rho Proteins Drives Cytoskeletal Remodeling in Spreading Endothelial Cells. International Journal of Molecular Sciences. 2021; 22(22):12417. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222212417
Chicago/Turabian StyleBarbera, Stefano, Luisa Raucci, Roberta Lugano, Gian Marco Tosi, Anna Dimberg, Annalisa Santucci, Federico Galvagni, and Maurizio Orlandini. 2021. "CD93 Signaling via Rho Proteins Drives Cytoskeletal Remodeling in Spreading Endothelial Cells" International Journal of Molecular Sciences 22, no. 22: 12417. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222212417