Aquaporin-1 Facilitates Transmesothelial Water Permeability: In Vitro and Ex Vivo Evidence and Possible Implications in Peritoneal Dialysis
Abstract
:1. Introduction
2. Results
2.1. Characterization of HMC
2.1.1. Immunofluorescence Confocal Analysis
2.1.2. Evaluation of HMC Monolayers Polarization
2.1.3. Electrophysiological Characterization of HMC Monolayers
2.2. Real Time Measurements of Transmesothelial Water Flux across the HMC Monolayers and Mouse Mesentery: Role of AQP1
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Cell Culture
4.3. Transepithelial Electrical Resistance Measurements
4.4. Immunofluorescence and Confocal Microscopy
4.5. Ussing Chamber Studies
4.6. Transmesothelial Water Flux Measurements
4.7. Data Analysis and Statistics
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Conflicts of Interest
Abbreviations
PD | Peritoneal Dialysis |
AQP1 | Aquaporin1 |
UF | Ultrafiltration |
TEER | TransEpithelial Electrical Resistance |
ECM | Extracellular matrix |
HMC | Human Mesothelial Cells |
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Piccapane, F.; Gerbino, A.; Carmosino, M.; Milano, S.; Arduini, A.; Debellis, L.; Svelto, M.; Caroppo, R.; Procino, G. Aquaporin-1 Facilitates Transmesothelial Water Permeability: In Vitro and Ex Vivo Evidence and Possible Implications in Peritoneal Dialysis. Int. J. Mol. Sci. 2021, 22, 12535. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222212535
Piccapane F, Gerbino A, Carmosino M, Milano S, Arduini A, Debellis L, Svelto M, Caroppo R, Procino G. Aquaporin-1 Facilitates Transmesothelial Water Permeability: In Vitro and Ex Vivo Evidence and Possible Implications in Peritoneal Dialysis. International Journal of Molecular Sciences. 2021; 22(22):12535. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222212535
Chicago/Turabian StylePiccapane, Francesca, Andrea Gerbino, Monica Carmosino, Serena Milano, Arduino Arduini, Lucantonio Debellis, Maria Svelto, Rosa Caroppo, and Giuseppe Procino. 2021. "Aquaporin-1 Facilitates Transmesothelial Water Permeability: In Vitro and Ex Vivo Evidence and Possible Implications in Peritoneal Dialysis" International Journal of Molecular Sciences 22, no. 22: 12535. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222212535