Isoprocurcumenol Supports Keratinocyte Growth and Survival through Epidermal Growth Factor Receptor Activation
Abstract
:1. Introduction
2. Results
2.1. Recognizing Isoprocurcumenol as an EGF Analogue
2.2. Measuring Appropriate Concentration of Isoprocurcumenol to Induce Cell Proliferation
2.3. IPC Induces the Expression of Genes Related to Cell Growth and Proliferation
2.4. IPC Induces Cell Recovery and Wound Healing
3. Discussion
4. Material and Methods
4.1. Cell Culture and Compound Treatment
4.2. SDS-Polyacrylamide Gel Electrophoresis (PAGE) and Immunoblotting
4.3. Cell Growth Assay
4.4. Cell Viability (MTT) Assay
4.5. RNA Extraction and Quantitative PCR
4.6. Wound Healing Assay
4.7. UVB Irradiation
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Kwon, P.K.; Kim, S.W.; De, R.; Jeong, S.W.; Kim, K.-T. Isoprocurcumenol Supports Keratinocyte Growth and Survival through Epidermal Growth Factor Receptor Activation. Int. J. Mol. Sci. 2021, 22, 12579. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222212579
Kwon PK, Kim SW, De R, Jeong SW, Kim K-T. Isoprocurcumenol Supports Keratinocyte Growth and Survival through Epidermal Growth Factor Receptor Activation. International Journal of Molecular Sciences. 2021; 22(22):12579. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222212579
Chicago/Turabian StyleKwon, Paul Kwangho, Sung Wook Kim, Ranjit De, Sung Woo Jeong, and Kyong-Tai Kim. 2021. "Isoprocurcumenol Supports Keratinocyte Growth and Survival through Epidermal Growth Factor Receptor Activation" International Journal of Molecular Sciences 22, no. 22: 12579. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222212579