Progressive Aggregation of 16 kDa Gamma-Zein during Seed Maturation in Transgenic Arabidopsis thaliana
Abstract
:1. Introduction
2. Results
2.1. 16γzf Accumulates in Arabidopsis Embryos without Interfering with the Synthesis and Processing of Endogenous Storage Proteins
2.2. 16γzf Avoids Traffic to PSVs and Forms Electron-Dense Threads That Progressively Coalesce during Embryo Development
2.3. 16γzf Forms Insoluble Aggregates in Mature Embryos, Unlike 27γzf
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Antibodies
4.3. Total Protein Extraction from Leaves, Roots, and Dry Seeds
4.4. Protein Body Precipitation
4.5. Protein Sequential Extraction with Different Buffer and Centrifugation
4.6. Electron Microscopy
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Arcalis, E.; Mainieri, D.; Vitale, A.; Stöger, E.; Pedrazzini, E. Progressive Aggregation of 16 kDa Gamma-Zein during Seed Maturation in Transgenic Arabidopsis thaliana. Int. J. Mol. Sci. 2021, 22, 12671. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222312671
Arcalis E, Mainieri D, Vitale A, Stöger E, Pedrazzini E. Progressive Aggregation of 16 kDa Gamma-Zein during Seed Maturation in Transgenic Arabidopsis thaliana. International Journal of Molecular Sciences. 2021; 22(23):12671. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222312671
Chicago/Turabian StyleArcalis, Elsa, Davide Mainieri, Alessandro Vitale, Eva Stöger, and Emanuela Pedrazzini. 2021. "Progressive Aggregation of 16 kDa Gamma-Zein during Seed Maturation in Transgenic Arabidopsis thaliana" International Journal of Molecular Sciences 22, no. 23: 12671. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222312671