Effect of 8-Day Fasting on Leukocytes Expression of Genes and Proteins Involved in Iron Metabolism in Healthy Men
Abstract
:1. Introduction
2. Results
2.1. Gene Expression and Oxidative Stress
2.1.1. Effect of 8 Days Starvation on Gene Expression
2.1.2. Effect of 8 Days starvation on 8-Isoprostane
2.1.3. Effects of Exercise on Gene Expression before and after 8 Days of Starvation
2.2. Immunoblotting
3. Discussion
4. Materials and Methods
4.1. Ethics
4.2. Study Group Characteristics
4.3. Anthropometrics
4.4. Exercise
4.5. Blood Sampling
4.6. Determination of Serum 8-Isoprostane
4.7. RNA Analysis
4.8. Reverse-Transcription and Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR)
4.9. Immunoblotting
4.10. Statistical Analysis
5. Conclusions
Study Limitation
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
APP | amyloid precursor protein |
BMI | body mass index |
BV | blood volume |
DMT1 | divalent metal transporter 1 |
FFM | fat-free mass |
FTH | ferritin heavy chain |
FTL | ferritin light chain |
FPN | ferroportin |
Hct | hematocrit |
Hb | hemoglobin |
IRP 1-2 | iron regulatory proteins 1-2 |
LIP | labile iron pool |
PCBP 1-4 | poly(rC)-binding protein 1-4 |
PV | plasma volume |
TBW | total body water; |
TFRC | transferrin receptor |
WBC | white blood cells |
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n = 13 | Baseline Values before Fasting | Baseline Values after Fasting | Δ(Before−after) | Cohen’s d (Effect Size) | |||
---|---|---|---|---|---|---|---|
Parameter | Mean | SD | Mean | SD | Mean | SD | |
Age | 50.3 ± 14.2 | ||||||
Body mass (kg) | 79.38 | 10.79 | 73.43 * | 10.26 | –5.96 | 0.79 | 0.57 |
Fat mass (kg) | 15.28 | 5.42 | 13.21 * | 5.33 | –2.07 | 0.56 | 0.39 |
FFM (kg) | 64.11 | 5.87 | 60.07 * | 5.88 | –4.04 | 0.89 | 0.69 |
TBW (kg) | 46.94 | 4.29 | 44.08 * | 4.11 | –2.86 | 0.62 | 0.68 |
BMI (kg/m2) | 24.89 | 3.52 | 23.02 * | 3.35 | –1.87 | 0.25 | 0.54 |
β-hydroxybutyrate (mmol/L) | 0.32 | 0.22 | 4.93 * | 0.65 | 3.74 | 1.94 | 9.5 |
Hb (g%) | 15.43 | 0.91 | 14.47 | 1.24 | –0.39 | 1.83 | 0.88 |
HCT (%) | 45.4 | 2.80 | 43.5 | 3.66 | –1.09 | 5.56 | 0.58 |
PV (%) | 100 | - | 98.54 | 7.06 | −1.46 | 7.06 | 0.28 |
BV (%) | 100 | - | 104.00 | 12.99 | 0.84 | 7.37 | −0.42 |
Gene | Forward Primer (5′–3′) | Reverse Primer (5′–3′) |
---|---|---|
TUBB NM_001293213 | TCCACGGCCTTGCTCTTGTTT | GACATCAAGGCGCATGTGAAC |
PCBP1 NM_006196 | AGAGTCATGACCATTCCGTAC | TCCTTGAATCGAGTAGGCATC |
PCBP2 NM_001128913 | TCCAGCTCTCCGGTCATCTTT | ACTGAATCCGGTGTTGCCATG |
PCBP3 NM_020528.3 | TGTGAAGAAGATGCGTGAGG | RGCTGTTGCTATGGAGTTGA |
PCBP4 NM_033010.2 | CCTGTCTAGAGACGGCCAAG | AGCAGGTGGTAAAGCCAAGA |
FTH1 NM_002032 | TCCTACGTTTACCTGTCCATG | CTGCAGCTTCATCAGTTTCTC |
FTL NM_000146 | GTCAATTTGTACCTGCAGGCC | CTCGGCCAATTCGCGGAA |
TFRC NM_001128148 | TGCAGCAGTGAGTCTCTTCA | AGGCCCATCTCCTTAACGAG |
APP NM_000484.4 | TCAGTCTCTCTCCCTGCTCT | GGTGGTTTTCGTTTCGTTTCGGTCA |
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Borkowska, A.; Tomczyk, M.; Żychowska, M.; Pilis, W.; Zych, M.; Antosiewicz, J. Effect of 8-Day Fasting on Leukocytes Expression of Genes and Proteins Involved in Iron Metabolism in Healthy Men. Int. J. Mol. Sci. 2021, 22, 3248. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22063248
Borkowska A, Tomczyk M, Żychowska M, Pilis W, Zych M, Antosiewicz J. Effect of 8-Day Fasting on Leukocytes Expression of Genes and Proteins Involved in Iron Metabolism in Healthy Men. International Journal of Molecular Sciences. 2021; 22(6):3248. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22063248
Chicago/Turabian StyleBorkowska, Andżelika, Maja Tomczyk, Małgorzata Żychowska, Wiesław Pilis, Michał Zych, and Jędrzej Antosiewicz. 2021. "Effect of 8-Day Fasting on Leukocytes Expression of Genes and Proteins Involved in Iron Metabolism in Healthy Men" International Journal of Molecular Sciences 22, no. 6: 3248. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22063248