Human MIKO-1, a Hybrid Protein That Regulates Macrophage Function, Suppresses Lung Fibrosis in a Mouse Model of Bleomycin-Induced Interstitial Lung Disease
Abstract
:1. Introduction
2. Results
2.1. hMIKO-1 Effectively Reduced Fibrosis in ILD Lung
2.2. hMIKO-1 Regulated the Genes Associated with the Anti-Inflammatory and Anti-Fibrotic Effects in ILD
2.3. hMIKO-1 Suppressed the Polarity of Mouse Macrophages to M2 Dominance In Vitro
3. Discussion
4. Materials and Methods
4.1. Ethics
4.2. Human MIKO-1
4.3. Animal Models and Surgical Procedure
4.4. Preparation of Mouse Macrophages and Co-Culture with hMIKO-1
4.5. Flow Cytometry
4.6. Enzyme-Linked Immunosorbent Assays
4.7. In Vitro Analysis of Murine Macrophages via qRT-PCR
4.8. Histological Evaluation and Assessment of the Fibrotic Area
4.9. Hydroxyproline Assay
4.10. qRT-PCR Analysis of the Lung In Vivo
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Kotani, T.; Ikemoto, M.; Matsuda, S.; Masutani, R.; Takeuchi, T. Human MIKO-1, a Hybrid Protein That Regulates Macrophage Function, Suppresses Lung Fibrosis in a Mouse Model of Bleomycin-Induced Interstitial Lung Disease. Int. J. Mol. Sci. 2022, 23, 9669. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms23179669
Kotani T, Ikemoto M, Matsuda S, Masutani R, Takeuchi T. Human MIKO-1, a Hybrid Protein That Regulates Macrophage Function, Suppresses Lung Fibrosis in a Mouse Model of Bleomycin-Induced Interstitial Lung Disease. International Journal of Molecular Sciences. 2022; 23(17):9669. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms23179669
Chicago/Turabian StyleKotani, Takuya, Masaki Ikemoto, Shogo Matsuda, Ryota Masutani, and Tohru Takeuchi. 2022. "Human MIKO-1, a Hybrid Protein That Regulates Macrophage Function, Suppresses Lung Fibrosis in a Mouse Model of Bleomycin-Induced Interstitial Lung Disease" International Journal of Molecular Sciences 23, no. 17: 9669. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms23179669