Cross-Linked Hyaluronate and Corticosteroid Combination Ameliorate the Rat Experimental Tendinopathy through Anti-Senescent and -Apoptotic Effects
Abstract
:1. Introduction
2. Results
2.1. Tenocyte Viability and Collagen Synthesis
2.2. Inflammatory Mediators and MMPs in Tenocytes
2.3. Senescence and Apoptosis in Tenocytes
2.4. In Vivo Therapeutic Effects on a Rat Tendinopathy Model
3. Discussion
4. Materials and Methods
4.1. Ethic Statement
4.2. Preparation of cHA and cHA + DEX Formulations
4.3. Primary Culture of Human Tenocytes
4.4. Cell Viability, Senescence and TUNEL Analyses
4.5. Collagenase-Induced Patellar Tendinopathy Model
4.6. Histopathological Analysis
4.7. Immunoblot and Immunohistochemical Analyses
4.8. Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR)
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ko, P.-Y.; Hsu, C.-C.; Chen, S.-Y.; Kuo, L.-C.; Su, W.-R.; Jou, I.-M.; Su, F.-C.; Wu, P.-T. Cross-Linked Hyaluronate and Corticosteroid Combination Ameliorate the Rat Experimental Tendinopathy through Anti-Senescent and -Apoptotic Effects. Int. J. Mol. Sci. 2022, 23, 9760. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms23179760
Ko P-Y, Hsu C-C, Chen S-Y, Kuo L-C, Su W-R, Jou I-M, Su F-C, Wu P-T. Cross-Linked Hyaluronate and Corticosteroid Combination Ameliorate the Rat Experimental Tendinopathy through Anti-Senescent and -Apoptotic Effects. International Journal of Molecular Sciences. 2022; 23(17):9760. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms23179760
Chicago/Turabian StyleKo, Po-Yen, Che-Chia Hsu, Shih-Yao Chen, Li-Chieh Kuo, Wei-Ren Su, I-Ming Jou, Fong-Chin Su, and Po-Ting Wu. 2022. "Cross-Linked Hyaluronate and Corticosteroid Combination Ameliorate the Rat Experimental Tendinopathy through Anti-Senescent and -Apoptotic Effects" International Journal of Molecular Sciences 23, no. 17: 9760. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms23179760