Characteristics and Fitness Analysis through Interspecific Hybrid Progenies of Transgenic Brassica napus and B. rapa L. ssp.
Abstract
:1. Introduction
2. Results
2.1. Cross-Compatibility of Each B. rapa ssp. with Transgenic B. napus
2.2. Morphological Characteristics and Relative Fitness of Parental Genotypes and Interspecific Hybrids
2.3. Chromosome Numbers of Interspecific Hybrids and Progenies
2.4. Assessment of Intergenomic Recombination and Their Progenies Validation by Using SSR Markers
3. Discussion
4. Materials and Methods
4.1. Plant Material and Growth Conditions
4.2. Hybridization of GM B. napus with Different B. rapa ssp.
4.3. Morphological Characteristics
4.4. Chromosome Numbers
4.5. SSR Analysis
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Crossing Materials | Cross-Combination | No. of Pollinated Flowers | No. of Pods | Pod-Setting Ratio (%) | Total No. of Seeds | Vivipary (%) | Empty Seeds (%) | Crossability Index Avg. of 10 Pods/Plant (No. of Seeds/Pods) |
---|---|---|---|---|---|---|---|---|
Parental | B. napus | 410 | 282 | 68.8 | 6073 | 89 (1.47) | 249 (4) | 21.5 ± 0.7 b |
GM B. napus | 1197 | 786 | 65.6 | 4772 | 53 | 123 | 10.1 ± 5.3 e | |
B. rapa ssp. pekinensis | 268 | 159 | 59.4 | 1657 | 12 (0.72) | 454 (27) | 10.6 ± 0.7 c | |
B. rapa ssp. parachinensis | 1741 | 641 | 36.8 | 4153 | 174 (4.2) | 610 (14.7) | 13.7 ± 1.2 d | |
B. rapa ssp. chinensis | 792 | 292 | 36.9 | 3723 | 161 (4.3) | 544 (14.6) | 25.1 ± 2.3 a | |
B. rapa ssp. nipposinica | 1535 | 421 | 27.4 | 2704 | 61 (2.3) | 873 (32.3) | 13.3 ± 1.1 c | |
B. rapa ssp. narinosa | 1211 | 656 | 54.2 | 5338 | 54 (1.0) | 2058 (38.6) | 19.8 ± 1.5 b | |
B. rapa ssp. oleifera | 1875 | 603 | 32.2 | 3799 | 158 (4.2) | 827 (21.8) | 15.0 ± 1.8 e | |
B. rapa ssp. rapa | 285 | 201 | 70.5 | 147 | 1 (0.7) | 14 (9.5) | 6.1 ± 1.2 c | |
F1 Hybrids | B. rapa ssp. pekinensis ♀ × GM B. napus♂ | 1282 | 540 | 42.1 | 1926 | 518 (26.9) | 403 (20.9) | 15.4 ± 1.7 ab |
B. rapa ssp. parachinensis♀ × GM B. napus ♂ | 220 | 155 | 70.5 | 1932 | 1138 (58.9) | 144 (7.5) | 15.4 ± 2.7 ab | |
B. rapa ssp. chinensis♀ × GM B. napus ♂ | 390 | 217 | 55.6 | 1758 | 1250 (71.1) | 385 (21.9) | 13.6 ± 1.5 abc | |
B. rapa ssp. nipposinica♀ × GM B. napus ♂ | 417 | 157 | 37.6 | 1054 | 769 (73) | 224 (21.3) | 12.3 ± 1.4 bc | |
B. rapa ssp. narinosa♀ × GM B. napus ♂ | 616 | 421 | 68.3 | 3718 | 1415 (38.1) | 734 (19.7) | 16.9 ± 2.6 a | |
B. rapa ssp. oleifera ♀ × GM B. napus | 703 | 411 | 58.5 | 3936 | 1145 (29.1) | 2498 (63.5) | 16.2 ± 2.8 a | |
B. rapa ssp. rapa ♀ × GM B. napus ♂ | 580 | 449 | 77.4 | 748 | 170 (22.7) | 370 (49.5) | 11.0 ± 2.8 c | |
Reciprocal Combinations | GM B. napus ♀ × B. rapa ssp. pekinensis ♂ | 843 | 380 | 45.1 | 2595 | 25 (1.0) | 75 (2.9) | 14.2 ± 1.1 bc |
GM B. napus ♀ × B. rapa ssp. parachinensis ♂ | 164 | 95 | 57.9 | 1522 | 21 (1.4) | 49 (3.2) | 24.4 ± 2.7 d | |
GM B. napus ♀ × B. rapa ssp. chinensis ♂ | 105 | 71 | 67.6 | 1112 | 1 (0.1) | 17 (1.5) | 22.1 ± 1.7 a | |
GM B. napus ♀ × B. rapa ssp. nipposinica ♂ | 175 | 107 | 61.1 | 1864 | 79 (4.2) | 81 (4.3) | 27.5 ± 2.9 c | |
GM B. napus ♀ × B. rapa ssp. narinosa ♂ | 169 | 119 | 70.4 | 1464 | 25 (1.7) | 42 (2.9) | 19.2 ± 1.6 b | |
GM B. napus ♀ × B. rapa ssp. oleifera ♂ | 167 | 117 | 70.1 | 1649 | 53 (3.2) | 36 (2.2) | 20.8 ± 2.4 d | |
GM B. napus ♀ × B. rapa ssp. rapa ♂ | 913 | 299 | 32.7 | 1315 | 33 (2.5) | 74 (5.6) | 11.9 ± 1.5 c | |
F1 Hybrid (Selfing) | B. rapa ssp. pekinensis♀ × GM B. napus ♂ | 4053 | - | - | - | - | - | - |
B. rapa ssp. parachinensis ♀ × GM B. napus ♂ | 1245 | 5 | 0.4 | 4 | 1 (25) | - | 0.8 ± 0.8 ab | |
B. rapa ssp. chinensis ♀ × GM B. napus ♂ | 1613 | 109 | 6.8 | 165 | 17 (10.3) | 51 (30.9) | 1.2 ± 0.4 a | |
B. rapa ssp. nipposinica ♀ × GM B. napus ♂ | 1415 | 4 | 0.3 | 3 | - | 2 (66.7) | 0.75 ± 0.5 a | |
B. rapa ssp. narinosa ♀ × GM B. napus ♂ | 2735 | 23 | 16.3 | 25 | 1 (4) | 9 (36) | 0.85 ± 0.6 a | |
B. rapa ssp. oleifera ♀ × GM B. napus ♂ | 1683 | 200 | 11.9 | 240 | 17 (7.1) | 147 (61.3) | 1.4 ± 0.5 b | |
B. rapa ssp. rapa ♀ × GM B. napus ♂ | 6382 | 551 | 8.63 | 877 | 61 (7) | 248 (28.3) | 1.6 ± 0.7 ab |
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Sohn, S.-I.; Thamilarasan, S.K.; Pandian, S.; Oh, Y.-J.; Kang, H.-J.; Shin, E.-K. Characteristics and Fitness Analysis through Interspecific Hybrid Progenies of Transgenic Brassica napus and B. rapa L. ssp. Int. J. Mol. Sci. 2022, 23, 10512. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms231810512
Sohn S-I, Thamilarasan SK, Pandian S, Oh Y-J, Kang H-J, Shin E-K. Characteristics and Fitness Analysis through Interspecific Hybrid Progenies of Transgenic Brassica napus and B. rapa L. ssp. International Journal of Molecular Sciences. 2022; 23(18):10512. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms231810512
Chicago/Turabian StyleSohn, Soo-In, Senthil Kumar Thamilarasan, Subramani Pandian, Young-Ju Oh, Hyeon-Jung Kang, and Eun-Kyoung Shin. 2022. "Characteristics and Fitness Analysis through Interspecific Hybrid Progenies of Transgenic Brassica napus and B. rapa L. ssp." International Journal of Molecular Sciences 23, no. 18: 10512. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms231810512