Phage-Based Control of Methicillin Resistant Staphylococcus aureus in a Galleria mellonella Model of Implant-Associated Infection
Abstract
:1. Introduction
2. Results
2.1. Bactericidal Activity of Sb-1 against MRSA ATCC 43300 Colonizing Stainless Steel K-Wires In Vitro
2.2. Development of G. mellonella Model of Implant-Associated Infection
2.3. Kinetic of Sb-1 Stability in G. mellonella Larvae
2.4. Sb-1 Activity versus MRSA ATCC 43300 in G. mellonella Model of Implant-Associated Infection
2.5. Ability of Sb-1 to Prevent K-Wire Colonization MRSA ATCC 43300 in G. mellonella Model of Implant-Associated Infection
3. Discussion
4. Materials and Methods
4.1. Bacterial Strain
4.2. Sb-1 Bacteriophage
4.3. Phage Plaque Assay
4.4. In Vitro Biofilm Formation of MRSA ATCC 43300 on Kirschner-Wires and Lytic Activity of Sb-1
4.5. Galleria Mellonella Model of Implant-Associated Infection
4.6. Sb-1 Efficacy to Reduce K-Wire Colonization in G. mellonella Model of Implant-Associated Infection
4.7. Ability of Sb-1 to Prevent K-Wire Colonization in a G. mellonella Model of Implant-Associated Infection
4.8. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Materazzi, A.; Bottai, D.; Campobasso, C.; Klatt, A.-B.; Cesta, N.; De Masi, M.; Trampuz, A.; Tavanti, A.; Di Luca, M. Phage-Based Control of Methicillin Resistant Staphylococcus aureus in a Galleria mellonella Model of Implant-Associated Infection. Int. J. Mol. Sci. 2022, 23, 14514. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms232314514
Materazzi A, Bottai D, Campobasso C, Klatt A-B, Cesta N, De Masi M, Trampuz A, Tavanti A, Di Luca M. Phage-Based Control of Methicillin Resistant Staphylococcus aureus in a Galleria mellonella Model of Implant-Associated Infection. International Journal of Molecular Sciences. 2022; 23(23):14514. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms232314514
Chicago/Turabian StyleMaterazzi, Alessandro, Daria Bottai, Claudia Campobasso, Ann-Brit Klatt, Novella Cesta, Margherita De Masi, Andrej Trampuz, Arianna Tavanti, and Mariagrazia Di Luca. 2022. "Phage-Based Control of Methicillin Resistant Staphylococcus aureus in a Galleria mellonella Model of Implant-Associated Infection" International Journal of Molecular Sciences 23, no. 23: 14514. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms232314514