SMILE Downregulation during Melanogenesis Induces MITF Transcription in B16F10 Cells
Abstract
:1. Introduction
2. Results
2.1. SMILE Inversely Correlates with MITF in Human Melanoma Biopsies
2.2. SMILE and MITF Are Reciprocally Regulated by α-MSH in B16F10 Mouse Melanoma Cells
2.3. SMILE Downregulates Melanogenesis in Melanoma Cells
2.4. SMILE Suppresses Transcription of MITF via Binding to CREB
3. Discussion
4. Materials and Methods
4.1. Dataset Preparation for SMILE and MITF Expression Analysis
4.2. Materials and Cell Culture
4.3. RNA Isolation and Analysis
4.4. Immunoblotting
4.5. Luciferase Reporter Assay
4.6. Determination of Melanin Content
4.7. Measurement of Tyrosinase Activity
4.8. Chromatin Immunoprecipitation Assay
4.9. Co-Immunoprecipitation
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Truong, X.T.; Lee, Y.-S.; Nguyen, T.T.P.; Kim, H.-J.; Kim, S.-H.; Moon, C.; Kim, D.-K.; Choi, H.-S.; Jeon, T.-I. SMILE Downregulation during Melanogenesis Induces MITF Transcription in B16F10 Cells. Int. J. Mol. Sci. 2022, 23, 15094. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms232315094
Truong XT, Lee Y-S, Nguyen TTP, Kim H-J, Kim S-H, Moon C, Kim D-K, Choi H-S, Jeon T-I. SMILE Downregulation during Melanogenesis Induces MITF Transcription in B16F10 Cells. International Journal of Molecular Sciences. 2022; 23(23):15094. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms232315094
Chicago/Turabian StyleTruong, Xuan T., Young-Seung Lee, Thuy T. P. Nguyen, Hyun-Jin Kim, Sung-Hak Kim, Changjong Moon, Don-Kyu Kim, Hueng-Sik Choi, and Tae-Il Jeon. 2022. "SMILE Downregulation during Melanogenesis Induces MITF Transcription in B16F10 Cells" International Journal of Molecular Sciences 23, no. 23: 15094. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms232315094