Mitochondrial Features and Expressions of MFN2 and DRP1 during Spermiogenesis in Phascolosoma esculenta
Abstract
:1. Introduction
2. Results
2.1. Mitochondrial Ultrastructure during Spermiogenesis
2.2. Expression Dynamics of MFN2 and DRP1 during Spermiogenesis
2.2.1. Full-Length cDNA of Pe-mfn2 and Pe-drp1 and Characteristics of Their Protein Structures
2.2.2. High Expression of MFN2 and DRP1 in Coelomic Fluid
2.2.3. High Expression of MFN2 and DRP1 during Breeding Season
2.2.4. High Expression of MFN2 and DRP1 in Coelomic Fluid Spermatid
2.2.5. Localization of MFN2 and DRP1 in Early Spermatids
2.2.6. Consistent Expression of MFN2 and DRP1 during Spermiogenesis
3. Discussion
3.1. Mitochondrial Distribution during Spermiogenesis
3.2. Morphological Evidence for the Role of Mitochondrial Dynamics during Spermatogenesis
3.3. Molecular Biology Evidence for a Role of Mitochondrial Dynamics during Spermatogenesis
4. Materials and Methods
4.1. Animals and Tissues
4.2. Transmission Electron Microscope
4.3. Immunofluorescence
4.4. Full-Length cDNA Cloning of mfn2 and drp1
4.4.1. RNA Extraction and Reverse Transcription
4.4.2. Full-Length cDNA Cloning
4.5. Analyses of Protein Structures and Properties
4.6. Quantitative Analysis of mfn2 and drp1 mRNA Expression
4.7. Western Blotting
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gao, X.; Feng, B.; Tang, D.; Du, C.; Hou, C.; Jin, S.; Zhu, J. Mitochondrial Features and Expressions of MFN2 and DRP1 during Spermiogenesis in Phascolosoma esculenta. Int. J. Mol. Sci. 2022, 23, 15517. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms232415517
Gao X, Feng B, Tang D, Du C, Hou C, Jin S, Zhu J. Mitochondrial Features and Expressions of MFN2 and DRP1 during Spermiogenesis in Phascolosoma esculenta. International Journal of Molecular Sciences. 2022; 23(24):15517. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms232415517
Chicago/Turabian StyleGao, Xinming, Binbin Feng, Daojun Tang, Chen Du, Congcong Hou, Shan Jin, and Junquan Zhu. 2022. "Mitochondrial Features and Expressions of MFN2 and DRP1 during Spermiogenesis in Phascolosoma esculenta" International Journal of Molecular Sciences 23, no. 24: 15517. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms232415517