Variability in the Response against Teladorsagia circumcincta in Lambs of Two Canarian Sheep Breeds
Abstract
:1. Introduction
2. Results
2.1. Parasitology
2.2. Humoral and Cellular Immune Response
2.3. Transcriptomic Analysis
3. Discussion
4. Materials and Methods
4.1. Animals and Parasitology
4.2. Enzyme-Linked Immunological Assay (ELISA)
4.3. Histology and Immunohistochemistry
4.4. RNA Extraction and Sequencing
4.5. RNA-Seq Quality Control and Alignment
4.6. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Breed | Individual Tag | Day 16 | Day 19 | Day 21 | Day 23 | Day 26 | Day 28 | Day 30 | Day 33 | Day 35 | Day 37 |
---|---|---|---|---|---|---|---|---|---|---|---|
CHB | 286 | 200 | 300 | 100 | 0 | 0 | 0 | 0 | 38 | 25 | 0 |
288 | 0 | 38 | 50 | 25 | 38 | 50 | 50 | 75 | 175 | 175 | |
289 | 200 | 900 | 850 | 650 | 413 | 475 | 650 | 1200 | 1200 | 1750 | |
290 | 0 | 0 | 25 | 50 | 113 | 200 | 200 | 225 | 250 | 350 | |
292 | 0 | 150 | 150 | 100 | 150 | 150 | 150 | 525 | 800 | 850 | |
295 | 50 | 38 | 0 | 0 | 38 | 50 | 125 | 675 | 600 | 950 | |
296 | 50 | 75 | 25 | 0 | 38 | 75 | 75 | 488 | 650 | 550 | |
297 | 0 | 0 | 0 | 0 | 0 | 25 | 25 | 75 | 75 | 325 | |
298 | 100 | 450 | 400 | 175 | 75 | 25 | 25 | 38 | 25 | 75 | |
299 | 50 | 75 | 25 | 0 | 0 | 0 | 0 | 0 | 25 | 50 | |
301 | 100 | 113 | 50 | 25 | 38 | 25 | 0 | 38 | 50 | 50 | |
302 | 75 | 113 | 75 | 50 | 225 | 175 | 125 | 188 | 425 | 750 | |
CS | 310 | 225 | 375 | 75 | 50 | 38 | 50 | 200 | 375 | 300 | 700 |
314 | 50 | 638 | 600 | 200 | 38 | 25 | 150 | 263 | 75 | 75 | |
315 | 325 | 900 | 800 | 1100 | 1425 | 600 | 650 | 1275 | 900 | 750 | |
316 | 150 | 600 | 400 | 150 | 113 | 75 | 150 | 300 | 300 | 225 | |
317 | 50 | 788 | 850 | 500 | 225 | 25 | 75 | 113 | 50 | 50 | |
320 | 75 | 113 | 75 | 75 | 38 | 100 | 150 | 150 | 75 | 75 | |
323 | 0 | 38 | 25 | 0 | 0 | 25 | 25 | 38 | 25 | 25 | |
324 | 200 | 300 | 125 | 50 | 263 | 200 | 75 | 75 | 75 | 100 | |
325 | 50 | 188 | 200 | 100 | 38 | 50 | 25 | 75 | 75 | 50 | |
326 | 25 | 38 | 50 | 50 | 38 | 0 | 25 | 38 | 25 | 75 | |
332 | 400 | 1200 | 1400 | 1550 | 1500 | 800 | 1300 | 1950 | 1000 | 1300 | |
500 | 0 | 0 | 0 | 0 | 38 | 50 | 25 | 38 | 50 | 50 |
Adult Worm Burden | Female Worm Length | Eggs in Utero | |
---|---|---|---|
Cumulative FEC | 0.654 ** | 0.476 * | 0.332 |
Adult worm burden | - | 0.464 * | 0.578 ** |
Female worm length | - | 0.501 * |
Correlation | |||||
---|---|---|---|---|---|
Variable | Mean (ODI or cells/mm2) ± SEM | Cumulative FEC | Adult Worm Burden | Female Worm Length | Eggs in Utero |
Mucus IgA-L3 | 1.10 ± 0.07 | −0.069 | −0.241 | −0.331 | −0.669 ** |
Mucus IgA-L4 | 1.15 ± 0.04 | −0.189 | −0.294 | −0.377 | −0.668 ** |
Mucus IgA-AD | 1.01 ± 0.02 | −0.141 | −0.543 ** | −0.175 | −0.419 * |
CD4+ | 41.01 ± 7.45 | −0.147 | 0.042 | −0.209 | 0.035 |
CD8+ | 74.21 ± 16.59 | −0.001 | −0.198 | 0.045 | 0.082 |
γδ+ | 18.05 ± 2.93 | −0.122 | −0.065 | −0.214 | −0.126 |
MHC-II+ | 33.21 ± 8.50 | −0.476 * | −0.476 * | −0.030 | −0.269 |
Gal 14+ | 4.00 ± 0.88 | −0.207 | −0.104 | −0.179 | −0.180 |
Eosinophils | 86.56 ± 10.73 | −0.336 | −0.375 | 0.126 | −0.314 |
Globule leukocytes | 172.70 ± 41.13 | −0.443 * | −0.350 | −0.287 | −0.323 |
Mast cells | 29.93 ± 6.91 | −0.129 | −0.137 | −0.159 | −0.251 |
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Pérez-Hernández, T.; Hernández, J.N.; Machín, C.; McNeilly, T.N.; Nisbet, A.J.; Matthews, J.B.; Burgess, S.T.G.; González, J.F. Variability in the Response against Teladorsagia circumcincta in Lambs of Two Canarian Sheep Breeds. Int. J. Mol. Sci. 2023, 24, 29. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms24010029
Pérez-Hernández T, Hernández JN, Machín C, McNeilly TN, Nisbet AJ, Matthews JB, Burgess STG, González JF. Variability in the Response against Teladorsagia circumcincta in Lambs of Two Canarian Sheep Breeds. International Journal of Molecular Sciences. 2023; 24(1):29. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms24010029
Chicago/Turabian StylePérez-Hernández, Tara, Julia N. Hernández, Cynthia Machín, Tom N. McNeilly, Alasdair J. Nisbet, Jacqueline B. Matthews, Stewart T. G. Burgess, and Jorge F. González. 2023. "Variability in the Response against Teladorsagia circumcincta in Lambs of Two Canarian Sheep Breeds" International Journal of Molecular Sciences 24, no. 1: 29. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms24010029